Diagnosis of human brucellosis caused by Brucella canis

The transmission of Brucella canis to man commonly occurs through contact with infected dogs or their secretions, or through direct laboratory exposure. The disease is underdiagnosed due to a general lack of serological testing facilities and misconceptions concerning its prevalence. This report sho...

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Detalhes bibliográficos
Autores: Lucero, Nidia E., Escobar, Gabriela I., Ayala, Sandra M., Jacob, Néstor R.
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2005
País:Argentina
Recursos:Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
Repositorio:Sistema de Gestión del Conocimiento ANLIS MALBRÁN
Idioma:inglés
OAI Identifier:oai:sgc.anlis.gob.ar:Publications/123456789/356
Acesso em linha:http://sgc.anlis.gob.ar/handle/123456789/356
http://jmm.sgmjournals.org/content/54/5/457.full.pdf
Access Level:acceso abierto
Palavra-chave:Brucelosis
Brucella canis
Humanos
Ensayo de Inmunoadsorción Enzimática
Zoonosis
Enfermedades de los Perros
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spelling Diagnosis of human brucellosis caused by Brucella canisLucero, Nidia E.Escobar, Gabriela I.Ayala, Sandra M.Jacob, Néstor R.BrucelosisBrucella canisHumanosEnsayo de Inmunoadsorción EnzimáticaZoonosisEnfermedades de los PerrosThe transmission of Brucella canis to man commonly occurs through contact with infected dogs or their secretions, or through direct laboratory exposure. The disease is underdiagnosed due to a general lack of serological testing facilities and misconceptions concerning its prevalence. This report shows the potential use of an indirect ELISA (IELISA) for the diagnosis of human brucellosis caused by B. canis in a population of patients negative by smooth-Brucella antigen tests but positive by rapid slide agglutination test (RSAT). One hundred and ten sera from asymptomatic people found negative by tests using smooth Brucella abortus antigen and by RSAT showed an IELISA specificity of 100 % when a cut-off value of 27 % positivity (%P) was selected. For 17 sera from patients with positive B. canis culture or in close contact with culture-positive dogs, the IELISA sensitivity was 100 % with the same cut-off value. The positive patients presented clinical symptoms similar to brucellosis caused by other species of Brucella and some of them received antibiotic treatment and made good progress. Using this cut-off value, we studied 35 patients with negative blood cultures but positive RSATs, and IELISA detected 18 as positive; of the 17 IELISA-negative, two were RSAT-positive at dilution 1 : 2 and 15 were weakly positive with pure serum. These samples were probably from patients at an early stage of infection or indicate false-positive results. No cross-reaction was observed among the sera from nine cases with a diagnosis other than brucellosis, but cross-reactivity was evident in sera from patients infected with smooth-Brucella species. Since routine brucellosis diagnosis does not include B. canis investigation, infection with this species may be more widespread than is currently suspected. The RSAT could be a suitable screening test for the diagnosis of B. canis human brucellosis, and a supplementary technique, such as IELISA, performed on all positive RSAT samples that were negative by B. abortus antigen could ensure diagnostic specificity and confirm the diagnosis.Fil: Lucero, Nidia E. ANLIS Dr.C.G.Malbrán. Laboratorio de Brucelosis; Argentina.Fil: Escobar, Gabriela I. ANLIS Dr.C.G.Malbrán. Laboratorio de Brucelosis; Argentina.Fil: Ayala, Sandra M. ANLIS Dr.C.G.Malbrán. Laboratorio de Brucelosis; Argentina.Fil: Jacob, Néstor R. Hospital Cosme Argerich. Infectologia; Argentina.2005info:ar-repo/semantics/articuloinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdf0022-2615http://sgc.anlis.gob.ar/handle/123456789/356http://jmm.sgmjournals.org/content/54/5/457.full.pdfJournal of Medical Microbiology, 2005, 54(5), 457–461.reponame:Sistema de Gestión del Conocimiento ANLIS MALBRÁNinstname:Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"instacron:ANLISenginfo:eu-repo/semantics/openAccess2024-05-10T11:51:36Zoai:sgc.anlis.gob.ar:Publications/123456789/356Institucionalhttp://sgc.anlis.gob.ar/Organismo científico-tecnológicoNo correspondehttp://sgc.anlis.gob.ar/oai/biblioteca@anlis.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:a2024-05-10 11:51:36.9Sistema de Gestión del Conocimiento ANLIS MALBRÁN - Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"false
dc.title.none.fl_str_mv Diagnosis of human brucellosis caused by Brucella canis
title Diagnosis of human brucellosis caused by Brucella canis
spellingShingle Diagnosis of human brucellosis caused by Brucella canis
Lucero, Nidia E.
Brucelosis
Brucella canis
Humanos
Ensayo de Inmunoadsorción Enzimática
Zoonosis
Enfermedades de los Perros
title_short Diagnosis of human brucellosis caused by Brucella canis
title_full Diagnosis of human brucellosis caused by Brucella canis
title_fullStr Diagnosis of human brucellosis caused by Brucella canis
title_full_unstemmed Diagnosis of human brucellosis caused by Brucella canis
title_sort Diagnosis of human brucellosis caused by Brucella canis
dc.creator.none.fl_str_mv Lucero, Nidia E.
Escobar, Gabriela I.
Ayala, Sandra M.
Jacob, Néstor R.
author Lucero, Nidia E.
author_facet Lucero, Nidia E.
Escobar, Gabriela I.
Ayala, Sandra M.
Jacob, Néstor R.
author_role author
author2 Escobar, Gabriela I.
Ayala, Sandra M.
Jacob, Néstor R.
author2_role author
author
author
dc.subject.none.fl_str_mv Brucelosis
Brucella canis
Humanos
Ensayo de Inmunoadsorción Enzimática
Zoonosis
Enfermedades de los Perros
topic Brucelosis
Brucella canis
Humanos
Ensayo de Inmunoadsorción Enzimática
Zoonosis
Enfermedades de los Perros
description The transmission of Brucella canis to man commonly occurs through contact with infected dogs or their secretions, or through direct laboratory exposure. The disease is underdiagnosed due to a general lack of serological testing facilities and misconceptions concerning its prevalence. This report shows the potential use of an indirect ELISA (IELISA) for the diagnosis of human brucellosis caused by B. canis in a population of patients negative by smooth-Brucella antigen tests but positive by rapid slide agglutination test (RSAT). One hundred and ten sera from asymptomatic people found negative by tests using smooth Brucella abortus antigen and by RSAT showed an IELISA specificity of 100 % when a cut-off value of 27 % positivity (%P) was selected. For 17 sera from patients with positive B. canis culture or in close contact with culture-positive dogs, the IELISA sensitivity was 100 % with the same cut-off value. The positive patients presented clinical symptoms similar to brucellosis caused by other species of Brucella and some of them received antibiotic treatment and made good progress. Using this cut-off value, we studied 35 patients with negative blood cultures but positive RSATs, and IELISA detected 18 as positive; of the 17 IELISA-negative, two were RSAT-positive at dilution 1 : 2 and 15 were weakly positive with pure serum. These samples were probably from patients at an early stage of infection or indicate false-positive results. No cross-reaction was observed among the sera from nine cases with a diagnosis other than brucellosis, but cross-reactivity was evident in sera from patients infected with smooth-Brucella species. Since routine brucellosis diagnosis does not include B. canis investigation, infection with this species may be more widespread than is currently suspected. The RSAT could be a suitable screening test for the diagnosis of B. canis human brucellosis, and a supplementary technique, such as IELISA, performed on all positive RSAT samples that were negative by B. abortus antigen could ensure diagnostic specificity and confirm the diagnosis.
publishDate 2005
dc.date.none.fl_str_mv 2005
dc.type.none.fl_str_mv info:ar-repo/semantics/articulo
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv 0022-2615
http://sgc.anlis.gob.ar/handle/123456789/356
http://jmm.sgmjournals.org/content/54/5/457.full.pdf
identifier_str_mv 0022-2615
url http://sgc.anlis.gob.ar/handle/123456789/356
http://jmm.sgmjournals.org/content/54/5/457.full.pdf
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Journal of Medical Microbiology, 2005, 54(5), 457–461.
reponame:Sistema de Gestión del Conocimiento ANLIS MALBRÁN
instname:Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
instacron:ANLIS
instname_str Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
instacron_str ANLIS
institution ANLIS
reponame_str Sistema de Gestión del Conocimiento ANLIS MALBRÁN
collection Sistema de Gestión del Conocimiento ANLIS MALBRÁN
repository.name.fl_str_mv Sistema de Gestión del Conocimiento ANLIS MALBRÁN - Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
repository.mail.fl_str_mv biblioteca@anlis.gov.ar
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score 15,811543

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