Differences in mitochondrial function between brain and heart of senile rats exposed to acute hypobaric hypoxia: Role of nitric oxide

Rat brain and heart display different endogenous protective responses against hypobaric hypoxia in an age-dependent way. The aim of the present work was to evaluate the effects of acute hypobaric hypoxia (HH, 48 h) on brain and heart mitochondrial function as well as the participation of nitric oxid...

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Detalles Bibliográficos
Autores: La Padula, P., Costa, Lidia Esther, Karadayian, Analia Graciela, Lores Arnaiz, Silvia, Czerniczyniec, Analia
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:Argentina
Institución:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/227668
Acceso en línea:http://hdl.handle.net/11336/227668
Access Level:acceso abierto
Palabra clave:AGING
BRAIN
HEART
HYPOBARIC HYPOXIA
MITOCHONDRIA
NITRIC OXIDE
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
id AR_c24c4c8b28ffe6ac0485ffe455fbcb8b
oai_identifier_str oai:ri.conicet.gov.ar:11336/227668
network_acronym_str AR
network_name_str Argentina
repository_id_str
dc.title.none.fl_str_mv Differences in mitochondrial function between brain and heart of senile rats exposed to acute hypobaric hypoxia: Role of nitric oxide
title Differences in mitochondrial function between brain and heart of senile rats exposed to acute hypobaric hypoxia: Role of nitric oxide
spellingShingle Differences in mitochondrial function between brain and heart of senile rats exposed to acute hypobaric hypoxia: Role of nitric oxide
La Padula, P.
AGING
BRAIN
HEART
HYPOBARIC HYPOXIA
MITOCHONDRIA
NITRIC OXIDE
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
title_short Differences in mitochondrial function between brain and heart of senile rats exposed to acute hypobaric hypoxia: Role of nitric oxide
title_full Differences in mitochondrial function between brain and heart of senile rats exposed to acute hypobaric hypoxia: Role of nitric oxide
title_fullStr Differences in mitochondrial function between brain and heart of senile rats exposed to acute hypobaric hypoxia: Role of nitric oxide
title_full_unstemmed Differences in mitochondrial function between brain and heart of senile rats exposed to acute hypobaric hypoxia: Role of nitric oxide
title_sort Differences in mitochondrial function between brain and heart of senile rats exposed to acute hypobaric hypoxia: Role of nitric oxide
dc.creator.none.fl_str_mv La Padula, P.
Costa, Lidia Esther
Karadayian, Analia Graciela
Lores Arnaiz, Silvia
Czerniczyniec, Analia
author La Padula, P.
author_facet La Padula, P.
Costa, Lidia Esther
Karadayian, Analia Graciela
Lores Arnaiz, Silvia
Czerniczyniec, Analia
author_role author
author2 Costa, Lidia Esther
Karadayian, Analia Graciela
Lores Arnaiz, Silvia
Czerniczyniec, Analia
author2_role author
author
author
author
dc.subject.none.fl_str_mv AGING
BRAIN
HEART
HYPOBARIC HYPOXIA
MITOCHONDRIA
NITRIC OXIDE
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
topic AGING
BRAIN
HEART
HYPOBARIC HYPOXIA
MITOCHONDRIA
NITRIC OXIDE
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
description Rat brain and heart display different endogenous protective responses against hypobaric hypoxia in an age-dependent way. The aim of the present work was to evaluate the effects of acute hypobaric hypoxia (HH, 48 h) on brain and heart mitochondrial function as well as the participation of nitric oxide (NO) in old rats (22-month old). Cortical mitochondria from rats exposed to HH decreased respiratory rates (37 %, state 3) and membrane potential (20 %), but NO and H2O2 production increased by 48 %, and 23 %, respectively. Hippocampal mitochondria preserved O2 consumption and H2O2 production, decreased membrane potential (18 %) and increased NO production (46 %). By contrast, HH decreased NO production (53 %) in mitochondria from left heart ventricles associated with increased cytochrome oxidase activity (39 %) and decreased NADPH oxidase activity (31 %). Also, a tendency to increase complex I-III (24 %) and complex II-III (65 %) activity was observed. In conclusion, after HH hippocampal and cortical mitochondria showed mild uncoupling and increased NO production. However, only the hippocampus preserved O2 consumption and H2O2 levels. Interestingly, heart mitochondria showed a decreased ROS production through increased cytochrome oxidase activity associated with a decrease in NO production. This may be interpreted as a self-protective mechanism against hypoxia.
publishDate 2023
dc.date.none.fl_str_mv 2023-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/227668
La Padula, P.; Costa, Lidia Esther; Karadayian, Analia Graciela; Lores Arnaiz, Silvia; Czerniczyniec, Analia; Differences in mitochondrial function between brain and heart of senile rats exposed to acute hypobaric hypoxia: Role of nitric oxide; Pergamon-Elsevier Science Ltd; Experimental Gerontology; 173; 1-2023; 1-9
0531-5565
CONICET Digital
CONICET
url http://hdl.handle.net/11336/227668
identifier_str_mv La Padula, P.; Costa, Lidia Esther; Karadayian, Analia Graciela; Lores Arnaiz, Silvia; Czerniczyniec, Analia; Differences in mitochondrial function between brain and heart of senile rats exposed to acute hypobaric hypoxia: Role of nitric oxide; Pergamon-Elsevier Science Ltd; Experimental Gerontology; 173; 1-2023; 1-9
0531-5565
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.exger.2023.112100
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1799195207033421824
spelling Differences in mitochondrial function between brain and heart of senile rats exposed to acute hypobaric hypoxia: Role of nitric oxideLa Padula, P.Costa, Lidia EstherKaradayian, Analia GracielaLores Arnaiz, SilviaCzerniczyniec, AnaliaAGINGBRAINHEARTHYPOBARIC HYPOXIAMITOCHONDRIANITRIC OXIDEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Rat brain and heart display different endogenous protective responses against hypobaric hypoxia in an age-dependent way. The aim of the present work was to evaluate the effects of acute hypobaric hypoxia (HH, 48 h) on brain and heart mitochondrial function as well as the participation of nitric oxide (NO) in old rats (22-month old). Cortical mitochondria from rats exposed to HH decreased respiratory rates (37 %, state 3) and membrane potential (20 %), but NO and H2O2 production increased by 48 %, and 23 %, respectively. Hippocampal mitochondria preserved O2 consumption and H2O2 production, decreased membrane potential (18 %) and increased NO production (46 %). By contrast, HH decreased NO production (53 %) in mitochondria from left heart ventricles associated with increased cytochrome oxidase activity (39 %) and decreased NADPH oxidase activity (31 %). Also, a tendency to increase complex I-III (24 %) and complex II-III (65 %) activity was observed. In conclusion, after HH hippocampal and cortical mitochondria showed mild uncoupling and increased NO production. However, only the hippocampus preserved O2 consumption and H2O2 levels. Interestingly, heart mitochondria showed a decreased ROS production through increased cytochrome oxidase activity associated with a decrease in NO production. This may be interpreted as a self-protective mechanism against hypoxia.Fil: La Padula, P.. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto Alberto C. Taquini de Investigaciones en Medicina Traslacional - Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Cardiológicas "Prof. Dr. Alberto C. Taquini". Instituto Alberto C. Taquini de Investigaciones en Medicina Traslacional; ArgentinaFil: Costa, Lidia Esther. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto Alberto C. Taquini de Investigaciones en Medicina Traslacional - Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Cardiológicas "Prof. Dr. Alberto C. Taquini". Instituto Alberto C. Taquini de Investigaciones en Medicina Traslacional; ArgentinaFil: Karadayian, Analia Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; ArgentinaFil: Lores Arnaiz, Silvia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; ArgentinaFil: Czerniczyniec, Analia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; ArgentinaPergamon-Elsevier Science Ltd2023-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/227668La Padula, P.; Costa, Lidia Esther; Karadayian, Analia Graciela; Lores Arnaiz, Silvia; Czerniczyniec, Analia; Differences in mitochondrial function between brain and heart of senile rats exposed to acute hypobaric hypoxia: Role of nitric oxide; Pergamon-Elsevier Science Ltd; Experimental Gerontology; 173; 1-2023; 1-90531-5565CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.exger.2023.112100info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T13:46:09Zoai:ri.conicet.gov.ar:11336/227668instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 13:46:09.792CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
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