Signal transduction pathways in FSH regulation of rat sertoli cell proliferation

The final number of Sertoli cells reached during the proliferative periods determines sperm production capacity in adulthood. It is well known that FSH is the major Sertoli cell mitogen; however, little is known about the signal transduction pathways that regulate the proliferation of Sertoli cells....

Descripción completa

Detalles Bibliográficos
Autores: Riera, Maria Fernanda, Regueira, Mariana, Galardo, Maria Noel Lujan, Pellizzari, Eliana Herminia, Meroni, Silvina Beatriz, Cigorraga, Selva Beatriz
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2012
País:Argentina
Institución:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/68062
Acceso en línea:http://hdl.handle.net/11336/68062
Access Level:acceso abierto
Palabra clave:Adenosine 5=-Monophosphate-Activated Protein Kinase
Follicle-Stimulating Hormone
Phosphatidylinositol 3-Kinase/Akt/ Mammalian Target of Rapamycin Complex 1
https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
id AR_bf8e4ceef2e4ac4fa1ab2479646f20ef
oai_identifier_str oai:ri.conicet.gov.ar:11336/68062
network_acronym_str AR
network_name_str Argentina
repository_id_str
dc.title.none.fl_str_mv Signal transduction pathways in FSH regulation of rat sertoli cell proliferation
title Signal transduction pathways in FSH regulation of rat sertoli cell proliferation
spellingShingle Signal transduction pathways in FSH regulation of rat sertoli cell proliferation
Riera, Maria Fernanda
Adenosine 5=-Monophosphate-Activated Protein Kinase
Follicle-Stimulating Hormone
Phosphatidylinositol 3-Kinase/Akt/ Mammalian Target of Rapamycin Complex 1
https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
title_short Signal transduction pathways in FSH regulation of rat sertoli cell proliferation
title_full Signal transduction pathways in FSH regulation of rat sertoli cell proliferation
title_fullStr Signal transduction pathways in FSH regulation of rat sertoli cell proliferation
title_full_unstemmed Signal transduction pathways in FSH regulation of rat sertoli cell proliferation
title_sort Signal transduction pathways in FSH regulation of rat sertoli cell proliferation
dc.creator.none.fl_str_mv Riera, Maria Fernanda
Regueira, Mariana
Galardo, Maria Noel Lujan
Pellizzari, Eliana Herminia
Meroni, Silvina Beatriz
Cigorraga, Selva Beatriz
author Riera, Maria Fernanda
author_facet Riera, Maria Fernanda
Regueira, Mariana
Galardo, Maria Noel Lujan
Pellizzari, Eliana Herminia
Meroni, Silvina Beatriz
Cigorraga, Selva Beatriz
author_role author
author2 Regueira, Mariana
Galardo, Maria Noel Lujan
Pellizzari, Eliana Herminia
Meroni, Silvina Beatriz
Cigorraga, Selva Beatriz
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Adenosine 5=-Monophosphate-Activated Protein Kinase
Follicle-Stimulating Hormone
Phosphatidylinositol 3-Kinase/Akt/ Mammalian Target of Rapamycin Complex 1
https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
topic Adenosine 5=-Monophosphate-Activated Protein Kinase
Follicle-Stimulating Hormone
Phosphatidylinositol 3-Kinase/Akt/ Mammalian Target of Rapamycin Complex 1
https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
description The final number of Sertoli cells reached during the proliferative periods determines sperm production capacity in adulthood. It is well known that FSH is the major Sertoli cell mitogen; however, little is known about the signal transduction pathways that regulate the proliferation of Sertoli cells. The hypothesis of this investigation was that FSH regulates proliferation through a PI3K/Akt/mTORC1 pathway, and additionally, AMPK-dependent mechanisms counteract FSH proliferative effects. The present study was performed in 8-day-old rat Sertoli cell cultures. The results presented herein show that FSH, in addition to increasing p-Akt, p-mTOR, and p-p70S6K levels, increases p-PRAS40 levels, probably contributing to improving mTORC1 signaling. Furthermore, the decrease in FSH-stimulated p-Akt, p-mTOR, p-p70S6K, and p-PRAS40 levels in the presence of wortmannin emphasizes the participation of PI3K in FSH signaling. Additionally, the inhibition of FSH-stimulated Sertoli cell proliferation by the effect of wortmannin and rapamycin point to the relevance of the PI3K/Akt/mTORC1 signaling pathway in the mitotic activity of FSH. On the other hand, by activating AMPK, several interesting observations were made. Activation of AMPK produced an increase in Raptor phosphorylation, a decrease in p70S6K phosphorylation, and a decrease in FSH-stimulated Sertoli cell proliferation. The decrease in FSH-stimulated cell proliferation was accompanied by an increased expression of the cyclin-dependent kinase inhibitors (CDKIs) p19INK4d, p21Cip1, and p27Kip1. In summary, it is concluded that FSH regulates Sertoli cell proliferation with the participation of a PI3K/Akt/mTORC1 pathway and that AMPK activation may be involved in the detention of proliferation by, at least in part, a decrease in mTORC1 signaling and an increase in CDKI expression.
publishDate 2012
dc.date.none.fl_str_mv 2012-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/68062
Riera, Maria Fernanda; Regueira, Mariana; Galardo, Maria Noel Lujan; Pellizzari, Eliana Herminia; Meroni, Silvina Beatriz; et al.; Signal transduction pathways in FSH regulation of rat sertoli cell proliferation; American Physiological Society; American Journal Of Physiology-endocrinology And Metabolism; 302; 8; 4-2012; 914-923
0193-1849
CONICET Digital
CONICET
url http://hdl.handle.net/11336/68062
identifier_str_mv Riera, Maria Fernanda; Regueira, Mariana; Galardo, Maria Noel Lujan; Pellizzari, Eliana Herminia; Meroni, Silvina Beatriz; et al.; Signal transduction pathways in FSH regulation of rat sertoli cell proliferation; American Physiological Society; American Journal Of Physiology-endocrinology And Metabolism; 302; 8; 4-2012; 914-923
0193-1849
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1152/ajpendo.00477.2011
info:eu-repo/semantics/altIdentifier/url/https://www.physiology.org/doi/full/10.1152/ajpendo.00477.2011
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Physiological Society
publisher.none.fl_str_mv American Physiological Society
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1799195285210005504
spelling Signal transduction pathways in FSH regulation of rat sertoli cell proliferationRiera, Maria FernandaRegueira, MarianaGalardo, Maria Noel LujanPellizzari, Eliana HerminiaMeroni, Silvina BeatrizCigorraga, Selva BeatrizAdenosine 5=-Monophosphate-Activated Protein KinaseFollicle-Stimulating HormonePhosphatidylinositol 3-Kinase/Akt/ Mammalian Target of Rapamycin Complex 1https://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3The final number of Sertoli cells reached during the proliferative periods determines sperm production capacity in adulthood. It is well known that FSH is the major Sertoli cell mitogen; however, little is known about the signal transduction pathways that regulate the proliferation of Sertoli cells. The hypothesis of this investigation was that FSH regulates proliferation through a PI3K/Akt/mTORC1 pathway, and additionally, AMPK-dependent mechanisms counteract FSH proliferative effects. The present study was performed in 8-day-old rat Sertoli cell cultures. The results presented herein show that FSH, in addition to increasing p-Akt, p-mTOR, and p-p70S6K levels, increases p-PRAS40 levels, probably contributing to improving mTORC1 signaling. Furthermore, the decrease in FSH-stimulated p-Akt, p-mTOR, p-p70S6K, and p-PRAS40 levels in the presence of wortmannin emphasizes the participation of PI3K in FSH signaling. Additionally, the inhibition of FSH-stimulated Sertoli cell proliferation by the effect of wortmannin and rapamycin point to the relevance of the PI3K/Akt/mTORC1 signaling pathway in the mitotic activity of FSH. On the other hand, by activating AMPK, several interesting observations were made. Activation of AMPK produced an increase in Raptor phosphorylation, a decrease in p70S6K phosphorylation, and a decrease in FSH-stimulated Sertoli cell proliferation. The decrease in FSH-stimulated cell proliferation was accompanied by an increased expression of the cyclin-dependent kinase inhibitors (CDKIs) p19INK4d, p21Cip1, and p27Kip1. In summary, it is concluded that FSH regulates Sertoli cell proliferation with the participation of a PI3K/Akt/mTORC1 pathway and that AMPK activation may be involved in the detention of proliferation by, at least in part, a decrease in mTORC1 signaling and an increase in CDKI expression.Fil: Riera, Maria Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas "Dr. César Bergada". Gobierno de la Ciudad de Buenos Aires. Centro de Investigaciones Endocrinológicas "Dr. César Bergada". Fundación de Endocrinología Infantil. Centro de Investigaciones Endocrinológicas "Dr. César Bergada"; ArgentinaFil: Regueira, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas "Dr. César Bergada". Gobierno de la Ciudad de Buenos Aires. Centro de Investigaciones Endocrinológicas "Dr. César Bergada". Fundación de Endocrinología Infantil. Centro de Investigaciones Endocrinológicas "Dr. César Bergada"; ArgentinaFil: Galardo, Maria Noel Lujan. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas "Dr. César Bergada". Gobierno de la Ciudad de Buenos Aires. Centro de Investigaciones Endocrinológicas "Dr. César Bergada". Fundación de Endocrinología Infantil. Centro de Investigaciones Endocrinológicas "Dr. César Bergada"; ArgentinaFil: Pellizzari, Eliana Herminia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas "Dr. César Bergada". Gobierno de la Ciudad de Buenos Aires. Centro de Investigaciones Endocrinológicas "Dr. César Bergada". Fundación de Endocrinología Infantil. Centro de Investigaciones Endocrinológicas "Dr. César Bergada"; ArgentinaFil: Meroni, Silvina Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas "Dr. César Bergada". Gobierno de la Ciudad de Buenos Aires. Centro de Investigaciones Endocrinológicas "Dr. César Bergada". Fundación de Endocrinología Infantil. Centro de Investigaciones Endocrinológicas "Dr. César Bergada"; ArgentinaFil: Cigorraga, Selva Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones Endocrinológicas "Dr. César Bergada". Gobierno de la Ciudad de Buenos Aires. Centro de Investigaciones Endocrinológicas "Dr. César Bergada". Fundación de Endocrinología Infantil. Centro de Investigaciones Endocrinológicas "Dr. César Bergada"; ArgentinaAmerican Physiological Society2012-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/68062Riera, Maria Fernanda; Regueira, Mariana; Galardo, Maria Noel Lujan; Pellizzari, Eliana Herminia; Meroni, Silvina Beatriz; et al.; Signal transduction pathways in FSH regulation of rat sertoli cell proliferation; American Physiological Society; American Journal Of Physiology-endocrinology And Metabolism; 302; 8; 4-2012; 914-9230193-1849CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1152/ajpendo.00477.2011info:eu-repo/semantics/altIdentifier/url/https://www.physiology.org/doi/full/10.1152/ajpendo.00477.2011info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T13:48:02Zoai:ri.conicet.gov.ar:11336/68062instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 13:48:03.204CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
score 15,812429

Similares en LA Referencia