Antimicrobial photodynamic polymeric films bearing biscarbazol triphenylamine end-capped dendrimeric Zn(II) porphyrin

A novel biscarbazol triphenylamine end-capped dendrimeric zinc(II) porphyrin (DP 5) was synthesized by click chemistry. This compound is a cruciform dendrimer that bears a nucleus of zinc(II) tetrapyrrolic macrocycle substituted at the meso positions by four identical substituents. These are formed...

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Autores: Heredia, Daniel Alejandro, Martinez, Sol Romina, Durantini, Andres Matías, Pérez, María Eugenia, Mangione, Maria Ines, Durantini, Javier Esteban, Gervaldo, Miguel Andres, Otero, Luis Alberto, Durantini, Edgardo Néstor
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2019
País:Argentina
Institución:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/119707
Acceso en línea:http://hdl.handle.net/11336/119707
Access Level:acceso abierto
Palabra clave:ANTIBACTERIAL SURFACE
CARBAZOLE
CLICK CHEMISTRY
PHOTODYNAMIC INACTIVATION
POLYMERIC FILMS
PORPHYRIN
https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
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oai_identifier_str oai:ri.conicet.gov.ar:11336/119707
network_acronym_str AR
network_name_str Argentina
repository_id_str
dc.title.none.fl_str_mv Antimicrobial photodynamic polymeric films bearing biscarbazol triphenylamine end-capped dendrimeric Zn(II) porphyrin
title Antimicrobial photodynamic polymeric films bearing biscarbazol triphenylamine end-capped dendrimeric Zn(II) porphyrin
spellingShingle Antimicrobial photodynamic polymeric films bearing biscarbazol triphenylamine end-capped dendrimeric Zn(II) porphyrin
Heredia, Daniel Alejandro
ANTIBACTERIAL SURFACE
CARBAZOLE
CLICK CHEMISTRY
PHOTODYNAMIC INACTIVATION
POLYMERIC FILMS
PORPHYRIN
https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
title_short Antimicrobial photodynamic polymeric films bearing biscarbazol triphenylamine end-capped dendrimeric Zn(II) porphyrin
title_full Antimicrobial photodynamic polymeric films bearing biscarbazol triphenylamine end-capped dendrimeric Zn(II) porphyrin
title_fullStr Antimicrobial photodynamic polymeric films bearing biscarbazol triphenylamine end-capped dendrimeric Zn(II) porphyrin
title_full_unstemmed Antimicrobial photodynamic polymeric films bearing biscarbazol triphenylamine end-capped dendrimeric Zn(II) porphyrin
title_sort Antimicrobial photodynamic polymeric films bearing biscarbazol triphenylamine end-capped dendrimeric Zn(II) porphyrin
dc.creator.none.fl_str_mv Heredia, Daniel Alejandro
Martinez, Sol Romina
Durantini, Andres Matías
Pérez, María Eugenia
Mangione, Maria Ines
Durantini, Javier Esteban
Gervaldo, Miguel Andres
Otero, Luis Alberto
Durantini, Edgardo Néstor
author Heredia, Daniel Alejandro
author_facet Heredia, Daniel Alejandro
Martinez, Sol Romina
Durantini, Andres Matías
Pérez, María Eugenia
Mangione, Maria Ines
Durantini, Javier Esteban
Gervaldo, Miguel Andres
Otero, Luis Alberto
Durantini, Edgardo Néstor
author_role author
author2 Martinez, Sol Romina
Durantini, Andres Matías
Pérez, María Eugenia
Mangione, Maria Ines
Durantini, Javier Esteban
Gervaldo, Miguel Andres
Otero, Luis Alberto
Durantini, Edgardo Néstor
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ANTIBACTERIAL SURFACE
CARBAZOLE
CLICK CHEMISTRY
PHOTODYNAMIC INACTIVATION
POLYMERIC FILMS
PORPHYRIN
https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
topic ANTIBACTERIAL SURFACE
CARBAZOLE
CLICK CHEMISTRY
PHOTODYNAMIC INACTIVATION
POLYMERIC FILMS
PORPHYRIN
https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
description A novel biscarbazol triphenylamine end-capped dendrimeric zinc(II) porphyrin (DP 5) was synthesized by click chemistry. This compound is a cruciform dendrimer that bears a nucleus of zinc(II) tetrapyrrolic macrocycle substituted at the meso positions by four identical substituents. These are formed by a tetrafluorophenyl group that possesses a triazole unit in the para position. This nitrogenous heterocyclic is connected to a 4,4′-di(N-carbazolyl)triphenylamine group by means of a phenylenevinylene bridge, which allows the conjugation between the nucleus and this external electropolymerizable carbazoyl group. In this structure, dendrimeric arms act as light-harvesting antennas, increasing the absorption of blue light, and as electroactive moieties. The electrochemical oxidation of the carbazole groups contained in the terminal arms of the DP 5 was used to obtain novel, stable, and reproducible fully π-conjugated photoactive polymeric films (FDP 5). First, the spectroscopic characteristics and photodynamic properties of DP 5 were compared with its constitutional components derived of porphyrin P 6 and carbazole D 7 moieties in solution. The fluorescence emissions of the dendrimeric units in DP 5 were more strongly quenched by the tetrapyrrolic macrocycle, indicating photoinduced energy transfer. In addition, FDP 5 film showed the Soret and Q absorption bands and red fluorescence emission of the corresponding zinc(II) porphyrin. Also, FDP 5 film was highly stable to photobleaching, and it was able to produce singlet molecular oxygen in both N,N-dimethylformamide (DMF) and water. Therefore, the porphyrin units embedded in the polymeric matrix of FDP 5 film mainly retain the photochemical properties. Photodynamic inactivation mediated by FDP 5 film was investigated in Staphylococcus aureus and Escherichia coli. When a cell suspension was deposited on the surface, complete eradication of S. aureus and a 99% reduction in E. coli survival were found after 15 and 30 min of irradiation, respectively. Also, FDP 5 film was highly effective to eliminate individual bacteria attached to the surface. In addition, photodynamic inactivation (PDI) sensitized by FDP 5 film produced >99.99% bacterial killing in biofilms formed on the surface after 60 min irradiation. The results indicate that FDP 5 film represents an interesting and versatile photodynamic active material to eradicate bacteria as planktonic cells, individual attached microbes, or biofilms.
publishDate 2019
dc.date.none.fl_str_mv 2019-07-16
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/119707
Heredia, Daniel Alejandro; Martinez, Sol Romina; Durantini, Andres Matías; Pérez, María Eugenia; Mangione, Maria Ines; et al.; Antimicrobial photodynamic polymeric films bearing biscarbazol triphenylamine end-capped dendrimeric Zn(II) porphyrin; American Chemical Society; ACS Applied Materials & Interfaces; 11; 31; 16-7-2019; 27574-27587
1944-8252
1944-8244
CONICET Digital
CONICET
url http://hdl.handle.net/11336/119707
identifier_str_mv Heredia, Daniel Alejandro; Martinez, Sol Romina; Durantini, Andres Matías; Pérez, María Eugenia; Mangione, Maria Ines; et al.; Antimicrobial photodynamic polymeric films bearing biscarbazol triphenylamine end-capped dendrimeric Zn(II) porphyrin; American Chemical Society; ACS Applied Materials & Interfaces; 11; 31; 16-7-2019; 27574-27587
1944-8252
1944-8244
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://pubs.acs.org/doi/10.1021/acsami.9b09119
info:eu-repo/semantics/altIdentifier/doi/ 10.1021/acsami.9b09119
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Chemical Society
publisher.none.fl_str_mv American Chemical Society
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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spelling Antimicrobial photodynamic polymeric films bearing biscarbazol triphenylamine end-capped dendrimeric Zn(II) porphyrinHeredia, Daniel AlejandroMartinez, Sol RominaDurantini, Andres MatíasPérez, María EugeniaMangione, Maria InesDurantini, Javier EstebanGervaldo, Miguel AndresOtero, Luis AlbertoDurantini, Edgardo NéstorANTIBACTERIAL SURFACECARBAZOLECLICK CHEMISTRYPHOTODYNAMIC INACTIVATIONPOLYMERIC FILMSPORPHYRINhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1A novel biscarbazol triphenylamine end-capped dendrimeric zinc(II) porphyrin (DP 5) was synthesized by click chemistry. This compound is a cruciform dendrimer that bears a nucleus of zinc(II) tetrapyrrolic macrocycle substituted at the meso positions by four identical substituents. These are formed by a tetrafluorophenyl group that possesses a triazole unit in the para position. This nitrogenous heterocyclic is connected to a 4,4′-di(N-carbazolyl)triphenylamine group by means of a phenylenevinylene bridge, which allows the conjugation between the nucleus and this external electropolymerizable carbazoyl group. In this structure, dendrimeric arms act as light-harvesting antennas, increasing the absorption of blue light, and as electroactive moieties. The electrochemical oxidation of the carbazole groups contained in the terminal arms of the DP 5 was used to obtain novel, stable, and reproducible fully π-conjugated photoactive polymeric films (FDP 5). First, the spectroscopic characteristics and photodynamic properties of DP 5 were compared with its constitutional components derived of porphyrin P 6 and carbazole D 7 moieties in solution. The fluorescence emissions of the dendrimeric units in DP 5 were more strongly quenched by the tetrapyrrolic macrocycle, indicating photoinduced energy transfer. In addition, FDP 5 film showed the Soret and Q absorption bands and red fluorescence emission of the corresponding zinc(II) porphyrin. Also, FDP 5 film was highly stable to photobleaching, and it was able to produce singlet molecular oxygen in both N,N-dimethylformamide (DMF) and water. Therefore, the porphyrin units embedded in the polymeric matrix of FDP 5 film mainly retain the photochemical properties. Photodynamic inactivation mediated by FDP 5 film was investigated in Staphylococcus aureus and Escherichia coli. When a cell suspension was deposited on the surface, complete eradication of S. aureus and a 99% reduction in E. coli survival were found after 15 and 30 min of irradiation, respectively. Also, FDP 5 film was highly effective to eliminate individual bacteria attached to the surface. In addition, photodynamic inactivation (PDI) sensitized by FDP 5 film produced >99.99% bacterial killing in biofilms formed on the surface after 60 min irradiation. The results indicate that FDP 5 film represents an interesting and versatile photodynamic active material to eradicate bacteria as planktonic cells, individual attached microbes, or biofilms.Fil: Heredia, Daniel Alejandro. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; ArgentinaFil: Martinez, Sol Romina. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; ArgentinaFil: Durantini, Andres Matías. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; ArgentinaFil: Pérez, María Eugenia. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; ArgentinaFil: Mangione, Maria Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Química Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Química Rosario; ArgentinaFil: Durantini, Javier Esteban. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados; ArgentinaFil: Gervaldo, Miguel Andres. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados; ArgentinaFil: Otero, Luis Alberto. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados; ArgentinaFil: Durantini, Edgardo Néstor. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; ArgentinaAmerican Chemical Society2019-07-16info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/119707Heredia, Daniel Alejandro; Martinez, Sol Romina; Durantini, Andres Matías; Pérez, María Eugenia; Mangione, Maria Ines; et al.; Antimicrobial photodynamic polymeric films bearing biscarbazol triphenylamine end-capped dendrimeric Zn(II) porphyrin; American Chemical Society; ACS Applied Materials & Interfaces; 11; 31; 16-7-2019; 27574-275871944-82521944-8244CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://pubs.acs.org/doi/10.1021/acsami.9b09119info:eu-repo/semantics/altIdentifier/doi/ 10.1021/acsami.9b09119info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T13:54:51Zoai:ri.conicet.gov.ar:11336/119707instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 13:54:52.233CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
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