Folate production and fol genes expression by the dairy starter culture Streptococcus thermophilus CRL803 in free and controlled pH batch fermentations
Folate production by Streptococcus thermophilus CRL 803 was examined during batch fermentations without pH control and at constant pH (6.0 and 5.0) at different incubation temperatures. Folate production was higher at 42 °C under both constant and non-constant pH fermentation conditions, the increas...
| Autores: | , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2017 |
| País: | Argentina |
| Institución: | Consejo Nacional de Investigaciones Científicas y Técnicas |
| Repositorio: | CONICET Digital (CONICET) |
| Idioma: | inglés |
| OAI Identifier: | oai:ri.conicet.gov.ar:11336/40079 |
| Acceso en línea: | http://hdl.handle.net/11336/40079 |
| Access Level: | acceso abierto |
| Palabra clave: | Culture Conditions Fol Genes Expression Folate Lactic Acid Bacteria Streptococcus Thermophilus https://purl.org/becyt/ford/2.11 https://purl.org/becyt/ford/2 |
| Sumario: | Folate production by Streptococcus thermophilus CRL 803 was examined during batch fermentations without pH control and at constant pH (6.0 and 5.0) at different incubation temperatures. Folate production was higher at 42 °C under both constant and non-constant pH fermentation conditions, the increase being more pronounced at constant pH 6.0. Folate production by S. thermophilus CRL 803 under different fermentation conditions was cell-growth-associated. These observations were supported by analysis at the transcriptional levels of fol genes in controlled pH of 6.0 and uncontrolled pH batch culture. At pH 6.0, the highest relative expression levels of folE, folQ, folK and folP genes (involved in de novo synthesis) were observed after 6 h of incubation. This is the first study on the expression of fol mRNA and their relation with folate synthesis by S. thermophilus, which is influenced by culture conditions. |
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