Heterologous expression of Arabidopsis ABF4 gene in potato enhances tuberization through ABA-GA crosstalk regulation

Potato (Solanum tuberosum L.) tuberization is regulated by many signals, such as abscisic acid (ABA),sucrose and gibberellic acid (GA). ABA and sucrose are positive modulators, while GA is an inhibitor of the process. ABF (ABRE-binding factor) proteins are transcription factors involved in ABA and s...

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Autores: Muñiz García, María Noelia, Stritzler, Margarita, Capiati, Daniela Andrea
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2014
País:Argentina
Recursos:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/3961
Acesso em linha:http://hdl.handle.net/11336/3961
Access Level:acceso abierto
Palavra-chave:Abf/Areb
Abf2
Abf4
Abscisic Acid
Giberellic Acid
Potato
Solanum Tuberosum
Tuberization
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
id AR_be49d7b3c7219f7f8d3dc15ef4cabdac
oai_identifier_str oai:ri.conicet.gov.ar:11336/3961
network_acronym_str AR
network_name_str Argentina
repository_id_str
spelling Heterologous expression of Arabidopsis ABF4 gene in potato enhances tuberization through ABA-GA crosstalk regulationMuñiz García, María NoeliaStritzler, MargaritaCapiati, Daniela AndreaAbf/ArebAbf2Abf4Abscisic AcidGiberellic AcidPotatoSolanum TuberosumTuberizationhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Potato (Solanum tuberosum L.) tuberization is regulated by many signals, such as abscisic acid (ABA),sucrose and gibberellic acid (GA). ABA and sucrose are positive modulators, while GA is an inhibitor of the process. ABF (ABRE-binding factor) proteins are transcription factors involved in ABA and stress signaling. Previously, we reported that S. tuberosum StABF1 could mediate the ABA effects on tuberization. The aim of the present study was to evaluate the potential use of ABF genes to enhance tuberization and to determine the molecular mechanism involved. For this purpose, transgenic potato plants expressing the Arabidopsis ABF4 or ABF2 genes were generated, and their tuberization capacity and response to tuberization-related signals were analyzed in vitro. The results indicate that both ABF4 and ABF2 proteins positively regulate potato tuber induction; however, only ABF4 expression significantly increases the number and weight of the tubers obtained, without stunting growth. ABF4 and ABF2 transgenic plants exhibit ABA hypersensitivity during tuberization, accompanied by a GA-deficient phenotype. ABF4 expression triggers a significant rise in ABA levels in stolons under tuber-inducing conditions as compared with wild-type plants and a transcriptional deregulation of GA metabolism genes. Our results demonstrate that Arabidopsis ABF4 functions in potato ABA-GA signaling crosstalk during tuberization by regulating the expression of ABA and GA-metabolism genes. ABF4 gene might be a potential tool to increase tuber production, since its heterologous expression in potato enhances tuber induction without affecting plant growth.Fil: Muñiz García, María Noelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular; ArgentinaFil: Stritzler, Margarita. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular; ArgentinaFil: Capiati, Daniela Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular; ArgentinaSpringer2014-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/3961Muñiz García, María Noelia; Stritzler, Margarita; Capiati, Daniela Andrea; Heterologous expression of Arabidopsis ABF4 gene in potato enhances tuberization through ABA-GA crosstalk regulation; Springer; Planta; 239; 3; 3-2014; 615-6310032-0935enginfo:eu-repo/semantics/altIdentifier/url/http://link.springer.com/article/10.1007%2Fs00425-013-2001-2info:eu-repo/semantics/altIdentifier/doi/10.1007/s00425-013-2001-2info:eu-repo/semantics/altIdentifier/issn/0032-0935info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T14:19:28Zoai:ri.conicet.gov.ar:11336/3961instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 14:19:29.104CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Heterologous expression of Arabidopsis ABF4 gene in potato enhances tuberization through ABA-GA crosstalk regulation
title Heterologous expression of Arabidopsis ABF4 gene in potato enhances tuberization through ABA-GA crosstalk regulation
spellingShingle Heterologous expression of Arabidopsis ABF4 gene in potato enhances tuberization through ABA-GA crosstalk regulation
Muñiz García, María Noelia
Abf/Areb
Abf2
Abf4
Abscisic Acid
Giberellic Acid
Potato
Solanum Tuberosum
Tuberization
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
title_short Heterologous expression of Arabidopsis ABF4 gene in potato enhances tuberization through ABA-GA crosstalk regulation
title_full Heterologous expression of Arabidopsis ABF4 gene in potato enhances tuberization through ABA-GA crosstalk regulation
title_fullStr Heterologous expression of Arabidopsis ABF4 gene in potato enhances tuberization through ABA-GA crosstalk regulation
title_full_unstemmed Heterologous expression of Arabidopsis ABF4 gene in potato enhances tuberization through ABA-GA crosstalk regulation
title_sort Heterologous expression of Arabidopsis ABF4 gene in potato enhances tuberization through ABA-GA crosstalk regulation
dc.creator.none.fl_str_mv Muñiz García, María Noelia
Stritzler, Margarita
Capiati, Daniela Andrea
author Muñiz García, María Noelia
author_facet Muñiz García, María Noelia
Stritzler, Margarita
Capiati, Daniela Andrea
author_role author
author2 Stritzler, Margarita
Capiati, Daniela Andrea
author2_role author
author
dc.subject.none.fl_str_mv Abf/Areb
Abf2
Abf4
Abscisic Acid
Giberellic Acid
Potato
Solanum Tuberosum
Tuberization
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
topic Abf/Areb
Abf2
Abf4
Abscisic Acid
Giberellic Acid
Potato
Solanum Tuberosum
Tuberization
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
description Potato (Solanum tuberosum L.) tuberization is regulated by many signals, such as abscisic acid (ABA),sucrose and gibberellic acid (GA). ABA and sucrose are positive modulators, while GA is an inhibitor of the process. ABF (ABRE-binding factor) proteins are transcription factors involved in ABA and stress signaling. Previously, we reported that S. tuberosum StABF1 could mediate the ABA effects on tuberization. The aim of the present study was to evaluate the potential use of ABF genes to enhance tuberization and to determine the molecular mechanism involved. For this purpose, transgenic potato plants expressing the Arabidopsis ABF4 or ABF2 genes were generated, and their tuberization capacity and response to tuberization-related signals were analyzed in vitro. The results indicate that both ABF4 and ABF2 proteins positively regulate potato tuber induction; however, only ABF4 expression significantly increases the number and weight of the tubers obtained, without stunting growth. ABF4 and ABF2 transgenic plants exhibit ABA hypersensitivity during tuberization, accompanied by a GA-deficient phenotype. ABF4 expression triggers a significant rise in ABA levels in stolons under tuber-inducing conditions as compared with wild-type plants and a transcriptional deregulation of GA metabolism genes. Our results demonstrate that Arabidopsis ABF4 functions in potato ABA-GA signaling crosstalk during tuberization by regulating the expression of ABA and GA-metabolism genes. ABF4 gene might be a potential tool to increase tuber production, since its heterologous expression in potato enhances tuber induction without affecting plant growth.
publishDate 2014
dc.date.none.fl_str_mv 2014-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/3961
Muñiz García, María Noelia; Stritzler, Margarita; Capiati, Daniela Andrea; Heterologous expression of Arabidopsis ABF4 gene in potato enhances tuberization through ABA-GA crosstalk regulation; Springer; Planta; 239; 3; 3-2014; 615-631
0032-0935
url http://hdl.handle.net/11336/3961
identifier_str_mv Muñiz García, María Noelia; Stritzler, Margarita; Capiati, Daniela Andrea; Heterologous expression of Arabidopsis ABF4 gene in potato enhances tuberization through ABA-GA crosstalk regulation; Springer; Planta; 239; 3; 3-2014; 615-631
0032-0935
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://link.springer.com/article/10.1007%2Fs00425-013-2001-2
info:eu-repo/semantics/altIdentifier/doi/10.1007/s00425-013-2001-2
info:eu-repo/semantics/altIdentifier/issn/0032-0935
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 15,812429

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