Activation of PPARγ reduces N-acetyl-cysteine -induced hypercorticoidism by down-regulating MC2R expression into adrenal glands

We previously demonstrated that oral supplementation with antioxidants induced hyperactivity of hypothalamus-pituitary-adrenal (HPA) axis, attested by hypercorticoidism, through an up-regulation of adrenocorticotrophic hormone (ACTH) receptors (MC2R) in adrenal. This study analyzed the role of perox...

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Autores: Ventura, Raíssa D., Chaves, Amanda S., Magalhães, Nathalia S., González, Florencia Belén, Pacini, María Florencia, Pérez Ransanz, Ana Rosa, Silva, Patrícia M.R., Martins, Marco A., Carvalho, Vinicius F.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2020
País:Argentina
Institución:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/145679
Acceso en línea:http://hdl.handle.net/11336/145679
Access Level:acceso abierto
Palabra clave:ANTIOXIDANT
GLUCOCORTICOIDS
HPA AXIS
PPAR-Γ
https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
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oai_identifier_str oai:ri.conicet.gov.ar:11336/145679
network_acronym_str AR
network_name_str Argentina
repository_id_str
dc.title.none.fl_str_mv Activation of PPARγ reduces N-acetyl-cysteine -induced hypercorticoidism by down-regulating MC2R expression into adrenal glands
title Activation of PPARγ reduces N-acetyl-cysteine -induced hypercorticoidism by down-regulating MC2R expression into adrenal glands
spellingShingle Activation of PPARγ reduces N-acetyl-cysteine -induced hypercorticoidism by down-regulating MC2R expression into adrenal glands
Ventura, Raíssa D.
ANTIOXIDANT
GLUCOCORTICOIDS
HPA AXIS
PPAR-Γ
https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
title_short Activation of PPARγ reduces N-acetyl-cysteine -induced hypercorticoidism by down-regulating MC2R expression into adrenal glands
title_full Activation of PPARγ reduces N-acetyl-cysteine -induced hypercorticoidism by down-regulating MC2R expression into adrenal glands
title_fullStr Activation of PPARγ reduces N-acetyl-cysteine -induced hypercorticoidism by down-regulating MC2R expression into adrenal glands
title_full_unstemmed Activation of PPARγ reduces N-acetyl-cysteine -induced hypercorticoidism by down-regulating MC2R expression into adrenal glands
title_sort Activation of PPARγ reduces N-acetyl-cysteine -induced hypercorticoidism by down-regulating MC2R expression into adrenal glands
dc.creator.none.fl_str_mv Ventura, Raíssa D.
Chaves, Amanda S.
Magalhães, Nathalia S.
González, Florencia Belén
Pacini, María Florencia
Pérez Ransanz, Ana Rosa
Silva, Patrícia M.R.
Martins, Marco A.
Carvalho, Vinicius F.
author Ventura, Raíssa D.
author_facet Ventura, Raíssa D.
Chaves, Amanda S.
Magalhães, Nathalia S.
González, Florencia Belén
Pacini, María Florencia
Pérez Ransanz, Ana Rosa
Silva, Patrícia M.R.
Martins, Marco A.
Carvalho, Vinicius F.
author_role author
author2 Chaves, Amanda S.
Magalhães, Nathalia S.
González, Florencia Belén
Pacini, María Florencia
Pérez Ransanz, Ana Rosa
Silva, Patrícia M.R.
Martins, Marco A.
Carvalho, Vinicius F.
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ANTIOXIDANT
GLUCOCORTICOIDS
HPA AXIS
PPAR-Γ
https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
topic ANTIOXIDANT
GLUCOCORTICOIDS
HPA AXIS
PPAR-Γ
https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
description We previously demonstrated that oral supplementation with antioxidants induced hyperactivity of hypothalamus-pituitary-adrenal (HPA) axis, attested by hypercorticoidism, through an up-regulation of adrenocorticotrophic hormone (ACTH) receptors (MC2R) in adrenal. This study analyzed the role of peroxisome proliferator-activated receptor (PPAR)-γ on HPA axis hyperactivity induced by N-acetyl-cysteine (NAC). Male Swiss-Webster mice were orally treated with NAC for 1, 3, 5, 10, 15, or 18 consecutive days. The PPAR-γ agonist rosiglitazone and/or antagonist GW9662 were daily-injected i.p. for 5 consecutive days, starting concomitantly with NAC treatment. Rosiglitazone treatment inhibited NAC-induced adrenal hypertrophy and hypercorticoidism. Rosiglitazone also significantly reversed the NAC-induced increase in the MC2R expression in adrenal, but not steroidogenic acute regulatory protein (StAR). NAC treatment reduces the expression of PPARγ in the adrenals, but rosiglitazone did not restore the expression of this cytoprotective gene. In addition, GW9662 blocked the ability of rosiglitazone to decrease plasma corticosterone levels in NAC-treated mice. In conclusion, our findings showed that antioxidant supplementation induced a state of hypercorticoidism through down-regulation of PPARγ expression in the adrenals, in a mechanism probably related to a down-regulation of ACTH receptor expression.
publishDate 2020
dc.date.none.fl_str_mv 2020-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/145679
Ventura, Raíssa D.; Chaves, Amanda S.; Magalhães, Nathalia S.; González, Florencia Belén; Pacini, María Florencia; et al.; Activation of PPARγ reduces N-acetyl-cysteine -induced hypercorticoidism by down-regulating MC2R expression into adrenal glands; Elsevier Science Inc.; Free Radical Biology and Medicine; 156; 8-2020; 137-143
0891-5849
CONICET Digital
CONICET
url http://hdl.handle.net/11336/145679
identifier_str_mv Ventura, Raíssa D.; Chaves, Amanda S.; Magalhães, Nathalia S.; González, Florencia Belén; Pacini, María Florencia; et al.; Activation of PPARγ reduces N-acetyl-cysteine -induced hypercorticoidism by down-regulating MC2R expression into adrenal glands; Elsevier Science Inc.; Free Radical Biology and Medicine; 156; 8-2020; 137-143
0891-5849
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0891584920311059
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.freeradbiomed.2020.06.008
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science Inc.
publisher.none.fl_str_mv Elsevier Science Inc.
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1799195468190711808
spelling Activation of PPARγ reduces N-acetyl-cysteine -induced hypercorticoidism by down-regulating MC2R expression into adrenal glandsVentura, Raíssa D.Chaves, Amanda S.Magalhães, Nathalia S.González, Florencia BelénPacini, María FlorenciaPérez Ransanz, Ana RosaSilva, Patrícia M.R.Martins, Marco A.Carvalho, Vinicius F.ANTIOXIDANTGLUCOCORTICOIDSHPA AXISPPAR-Γhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3We previously demonstrated that oral supplementation with antioxidants induced hyperactivity of hypothalamus-pituitary-adrenal (HPA) axis, attested by hypercorticoidism, through an up-regulation of adrenocorticotrophic hormone (ACTH) receptors (MC2R) in adrenal. This study analyzed the role of peroxisome proliferator-activated receptor (PPAR)-γ on HPA axis hyperactivity induced by N-acetyl-cysteine (NAC). Male Swiss-Webster mice were orally treated with NAC for 1, 3, 5, 10, 15, or 18 consecutive days. The PPAR-γ agonist rosiglitazone and/or antagonist GW9662 were daily-injected i.p. for 5 consecutive days, starting concomitantly with NAC treatment. Rosiglitazone treatment inhibited NAC-induced adrenal hypertrophy and hypercorticoidism. Rosiglitazone also significantly reversed the NAC-induced increase in the MC2R expression in adrenal, but not steroidogenic acute regulatory protein (StAR). NAC treatment reduces the expression of PPARγ in the adrenals, but rosiglitazone did not restore the expression of this cytoprotective gene. In addition, GW9662 blocked the ability of rosiglitazone to decrease plasma corticosterone levels in NAC-treated mice. In conclusion, our findings showed that antioxidant supplementation induced a state of hypercorticoidism through down-regulation of PPARγ expression in the adrenals, in a mechanism probably related to a down-regulation of ACTH receptor expression.Fil: Ventura, Raíssa D.. Fundación Oswaldo Cruz; BrasilFil: Chaves, Amanda S.. Fundación Oswaldo Cruz; BrasilFil: Magalhães, Nathalia S.. Fundación Oswaldo Cruz; BrasilFil: González, Florencia Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Inmunología Clinica y Experimental de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Médicas. Instituto de Inmunología Clinica y Experimental de Rosario; ArgentinaFil: Pacini, María Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Inmunología Clinica y Experimental de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Médicas. Instituto de Inmunología Clinica y Experimental de Rosario; ArgentinaFil: Pérez Ransanz, Ana Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Inmunología Clinica y Experimental de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Médicas. Instituto de Inmunología Clinica y Experimental de Rosario; ArgentinaFil: Silva, Patrícia M.R.. Fundación Oswaldo Cruz; BrasilFil: Martins, Marco A.. Fundación Oswaldo Cruz; BrasilFil: Carvalho, Vinicius F.. Fundación Oswaldo Cruz; BrasilElsevier Science Inc.2020-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/145679Ventura, Raíssa D.; Chaves, Amanda S.; Magalhães, Nathalia S.; González, Florencia Belén; Pacini, María Florencia; et al.; Activation of PPARγ reduces N-acetyl-cysteine -induced hypercorticoidism by down-regulating MC2R expression into adrenal glands; Elsevier Science Inc.; Free Radical Biology and Medicine; 156; 8-2020; 137-1430891-5849CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0891584920311059info:eu-repo/semantics/altIdentifier/doi/10.1016/j.freeradbiomed.2020.06.008info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T13:53:26Zoai:ri.conicet.gov.ar:11336/145679instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 13:53:26.569CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
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