Precipitation of chymotrypsin from fresh bovine pancreas using i-carrageenan
The separation of chymotrypsin from a crude filtrate of fresh bovine pancreas homogenate was carried out using precipitation with a commercially available negatively charged natural strong polyelectrolyte: -carrageenan. The zymogen form of the enzyme was activated by addition of trypsin (0.0001%, w/...
| Autores: | , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2012 |
| País: | Argentina |
| Institución: | Consejo Nacional de Investigaciones Científicas y Técnicas |
| Repositorio: | CONICET Digital (CONICET) |
| Idioma: | inglés |
| OAI Identifier: | oai:ri.conicet.gov.ar:11336/104546 |
| Acceso en línea: | http://hdl.handle.net/11336/104546 |
| Access Level: | acceso abierto |
| Palabra clave: | CARRAGEENAN CHYMOTRYPSIN POLYELECTROLYTE https://purl.org/becyt/ford/2.9 https://purl.org/becyt/ford/2 |
| Sumario: | The separation of chymotrypsin from a crude filtrate of fresh bovine pancreas homogenate was carried out using precipitation with a commercially available negatively charged natural strong polyelectrolyte: -carrageenan. The zymogen form of the enzyme was activated by addition of trypsin (0.0001%, w/w), then, the enzyme was precipitated by polyelectrolyte addition at pH 4.50. The non-soluble complex was separated by simple centrifugation and re-dissolved by a pH change to 8.20. The recovery of chymotrypsin biological activity was 60% of the initial activity in the homogenate with 3-fold increase in its specific activity. The volume of the final product decreased to 10% of the feedstock, concentrating the sample up to 10 times. |
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