Evaluation of the polymerase chain reaction for the detection of Mycobacterium tuberculosis

The development of nucleic acid-based technologies has improved the sensitivity, specificity and speed of detection of Mycobacterium tuberculosis in clinical samples. Both commercially available and ´in-house´ polymerase chain reaction (PCR) systems are in use, and a significant number of reports co...

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Autores: Suffys, P., Palomino, J. C., Cardoso Leão, S., Espitia, C., Cataldi, Ángel Adrián, Alito, A., Velasco, M., Robledo, J,, Fernandez, J., Rosa, P. da Silva, Romano, Maria Isabel
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2000
País:Argentina
Institución:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/71869
Acceso en línea:http://hdl.handle.net/11336/71869
Access Level:acceso abierto
Palabra clave:Tuberculosis
Pcr
Mycobacterium Tuberculosis
Multicenter
https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
id AR_b9d32c1aaa540bf4fda7f920daab4355
oai_identifier_str oai:ri.conicet.gov.ar:11336/71869
network_acronym_str AR
network_name_str Argentina
repository_id_str
dc.title.none.fl_str_mv Evaluation of the polymerase chain reaction for the detection of Mycobacterium tuberculosis
title Evaluation of the polymerase chain reaction for the detection of Mycobacterium tuberculosis
spellingShingle Evaluation of the polymerase chain reaction for the detection of Mycobacterium tuberculosis
Suffys, P.
Tuberculosis
Pcr
Mycobacterium Tuberculosis
Multicenter
https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
title_short Evaluation of the polymerase chain reaction for the detection of Mycobacterium tuberculosis
title_full Evaluation of the polymerase chain reaction for the detection of Mycobacterium tuberculosis
title_fullStr Evaluation of the polymerase chain reaction for the detection of Mycobacterium tuberculosis
title_full_unstemmed Evaluation of the polymerase chain reaction for the detection of Mycobacterium tuberculosis
title_sort Evaluation of the polymerase chain reaction for the detection of Mycobacterium tuberculosis
dc.creator.none.fl_str_mv Suffys, P.
Palomino, J. C.
Cardoso Leão, S.
Espitia, C.
Cataldi, Ángel Adrián
Alito, A.
Velasco, M.
Robledo, J,
Fernandez, J.
Rosa, P. da Silva
Romano, Maria Isabel
author Suffys, P.
author_facet Suffys, P.
Palomino, J. C.
Cardoso Leão, S.
Espitia, C.
Cataldi, Ángel Adrián
Alito, A.
Velasco, M.
Robledo, J,
Fernandez, J.
Rosa, P. da Silva
Romano, Maria Isabel
author_role author
author2 Palomino, J. C.
Cardoso Leão, S.
Espitia, C.
Cataldi, Ángel Adrián
Alito, A.
Velasco, M.
Robledo, J,
Fernandez, J.
Rosa, P. da Silva
Romano, Maria Isabel
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Tuberculosis
Pcr
Mycobacterium Tuberculosis
Multicenter
https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
topic Tuberculosis
Pcr
Mycobacterium Tuberculosis
Multicenter
https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
description The development of nucleic acid-based technologies has improved the sensitivity, specificity and speed of detection of Mycobacterium tuberculosis in clinical samples. Both commercially available and ´in-house´ polymerase chain reaction (PCR) systems are in use, and a significant number of reports compare such systems with more traditional diagnostic tools for tuberculosis. Few studies, however, have focused on the reproducibility of the results when submitting a sample batch to PCR in different laboratories, especially in developing countries. Consequently, PCR results obtained from six laboratories in six different Latin American countries for samples reconstituted with defined amounts of M. tuberculosis cells were evaluated. Each laboratory used specific conditions of sample processing, nucleic acid amplification and amplicon detection. Analysis of results allowed large differences in sensitivity and specificity to be observed. We conclude that in its present setting, in-house PCR cannot be used as a single diagnostic tool for tuberculosis, and that special care needs to be taken upon interpretation of results by inclusion of a proper number of positive and negative controls.
publishDate 2000
dc.date.none.fl_str_mv 2000-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/71869
Suffys, P.; Palomino, J. C.; Cardoso Leão, S.; Espitia, C. ; Cataldi, Ángel Adrián; et al.; Evaluation of the polymerase chain reaction for the detection of Mycobacterium tuberculosis; International Union Against Tuberculosis and Lung Disease; International Journal of Tuberculosis and Lung Disease; 4; 2-2000; 179-183
1027-3719
CONICET Digital
CONICET
url http://hdl.handle.net/11336/71869
identifier_str_mv Suffys, P.; Palomino, J. C.; Cardoso Leão, S.; Espitia, C. ; Cataldi, Ángel Adrián; et al.; Evaluation of the polymerase chain reaction for the detection of Mycobacterium tuberculosis; International Union Against Tuberculosis and Lung Disease; International Journal of Tuberculosis and Lung Disease; 4; 2-2000; 179-183
1027-3719
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.ingentaconnect.com/content/iuatld/ijtld/2000/00000004/00000002/art00015%3bjsessionid=3n9f6c9hox3uf.x-ic-live-02
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv International Union Against Tuberculosis and Lung Disease
publisher.none.fl_str_mv International Union Against Tuberculosis and Lung Disease
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1799196466976129024
spelling Evaluation of the polymerase chain reaction for the detection of Mycobacterium tuberculosisSuffys, P.Palomino, J. C.Cardoso Leão, S.Espitia, C.Cataldi, Ángel AdriánAlito, A.Velasco, M.Robledo, J,Fernandez, J.Rosa, P. da SilvaRomano, Maria IsabelTuberculosisPcrMycobacterium TuberculosisMulticenterhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3The development of nucleic acid-based technologies has improved the sensitivity, specificity and speed of detection of Mycobacterium tuberculosis in clinical samples. Both commercially available and ´in-house´ polymerase chain reaction (PCR) systems are in use, and a significant number of reports compare such systems with more traditional diagnostic tools for tuberculosis. Few studies, however, have focused on the reproducibility of the results when submitting a sample batch to PCR in different laboratories, especially in developing countries. Consequently, PCR results obtained from six laboratories in six different Latin American countries for samples reconstituted with defined amounts of M. tuberculosis cells were evaluated. Each laboratory used specific conditions of sample processing, nucleic acid amplification and amplicon detection. Analysis of results allowed large differences in sensitivity and specificity to be observed. We conclude that in its present setting, in-house PCR cannot be used as a single diagnostic tool for tuberculosis, and that special care needs to be taken upon interpretation of results by inclusion of a proper number of positive and negative controls.Fil: Suffys, P.. Fundación Oswaldo Cruz; BrasilFil: Palomino, J. C.. Universidad Peruana Cayetano Heredia; PerúFil: Cardoso Leão, S.. Universidade Federal de São Paulo; BrasilFil: Espitia, C.. Universidad Nacional Autónoma de México; MéxicoFil: Cataldi, Ángel Adrián. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; ArgentinaFil: Alito, A.. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; ArgentinaFil: Velasco, M.. Instituto de Salud Pública de Chile. Seccion Micobacterias y Unidad de Biología Molecular; ChileFil: Robledo, J,. Corporación para Investigaciones Biológicas; ColombiaFil: Fernandez, J.. Corporación para Investigaciones Biológicas; ColombiaFil: Rosa, P. da Silva. Fundación Oswaldo Cruz; BrasilFil: Romano, Maria Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; ArgentinaInternational Union Against Tuberculosis and Lung Disease2000-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/71869Suffys, P.; Palomino, J. C.; Cardoso Leão, S.; Espitia, C. ; Cataldi, Ángel Adrián; et al.; Evaluation of the polymerase chain reaction for the detection of Mycobacterium tuberculosis; International Union Against Tuberculosis and Lung Disease; International Journal of Tuberculosis and Lung Disease; 4; 2-2000; 179-1831027-3719CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.ingentaconnect.com/content/iuatld/ijtld/2000/00000004/00000002/art00015%3bjsessionid=3n9f6c9hox3uf.x-ic-live-02info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T14:28:55Zoai:ri.conicet.gov.ar:11336/71869instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 14:28:56.143CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
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