Modulation of Apoptosis by Bovine Gammaherpesvirus 4 Infection in Bovine Endometrial Cells and the Possible Role of LPS in This Process
The prevalent pathogens associated with bovine uterine infections are bacteria that appear to increase the host’s susceptibility to secondary infections with other bacteria or viruses, among which BoGHV4 is the most frequently found. In this work, the study of the pathways of apoptosis induction was...
| Autores: | , , , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2024 |
| País: | Argentina |
| Institución: | Instituto Nacional de Tecnología Agropecuaria |
| Repositorio: | INTA Digital (INTA) |
| Idioma: | inglés |
| OAI Identifier: | oai:localhost:20.500.12123/17390 |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/17390 https://www.mdpi.com/2079-7737/13/4/249 https://doi.org/10.3390/biology13040249 |
| Access Level: | acceso abierto |
| Palabra clave: | Herpes Virus Bovino Infección Muerte Célular Bovine Herpesvirus Apoptosis Infection Cell Death Bovinae |
| id |
AR_b4c2eaacc930973fa84da7bca81d538e |
|---|---|
| oai_identifier_str |
oai:localhost:20.500.12123/17390 |
| network_acronym_str |
AR |
| network_name_str |
Argentina |
| repository_id_str |
|
| dc.title.none.fl_str_mv |
Modulation of Apoptosis by Bovine Gammaherpesvirus 4 Infection in Bovine Endometrial Cells and the Possible Role of LPS in This Process |
| title |
Modulation of Apoptosis by Bovine Gammaherpesvirus 4 Infection in Bovine Endometrial Cells and the Possible Role of LPS in This Process |
| spellingShingle |
Modulation of Apoptosis by Bovine Gammaherpesvirus 4 Infection in Bovine Endometrial Cells and the Possible Role of LPS in This Process Romeo, Florencia Herpes Virus Bovino Infección Muerte Célular Bovine Herpesvirus Apoptosis Infection Cell Death Bovinae |
| title_short |
Modulation of Apoptosis by Bovine Gammaherpesvirus 4 Infection in Bovine Endometrial Cells and the Possible Role of LPS in This Process |
| title_full |
Modulation of Apoptosis by Bovine Gammaherpesvirus 4 Infection in Bovine Endometrial Cells and the Possible Role of LPS in This Process |
| title_fullStr |
Modulation of Apoptosis by Bovine Gammaherpesvirus 4 Infection in Bovine Endometrial Cells and the Possible Role of LPS in This Process |
| title_full_unstemmed |
Modulation of Apoptosis by Bovine Gammaherpesvirus 4 Infection in Bovine Endometrial Cells and the Possible Role of LPS in This Process |
| title_sort |
Modulation of Apoptosis by Bovine Gammaherpesvirus 4 Infection in Bovine Endometrial Cells and the Possible Role of LPS in This Process |
| dc.creator.none.fl_str_mv |
Romeo, Florencia Delgado, Santiago Germán Yavorsky, Marisol Martinez Cuesta, Lucía Pereyra, Susana Beatriz González Altamiranda, Erika Louge Uriarte, Enrique Leopoldo Pérez, Sandra Verna, Andrea Elizabeth |
| author |
Romeo, Florencia |
| author_facet |
Romeo, Florencia Delgado, Santiago Germán Yavorsky, Marisol Martinez Cuesta, Lucía Pereyra, Susana Beatriz González Altamiranda, Erika Louge Uriarte, Enrique Leopoldo Pérez, Sandra Verna, Andrea Elizabeth |
| author_role |
author |
| author2 |
Delgado, Santiago Germán Yavorsky, Marisol Martinez Cuesta, Lucía Pereyra, Susana Beatriz González Altamiranda, Erika Louge Uriarte, Enrique Leopoldo Pérez, Sandra Verna, Andrea Elizabeth |
| author2_role |
author author author author author author author author |
| dc.subject.none.fl_str_mv |
Herpes Virus Bovino Infección Muerte Célular Bovine Herpesvirus Apoptosis Infection Cell Death Bovinae |
| topic |
Herpes Virus Bovino Infección Muerte Célular Bovine Herpesvirus Apoptosis Infection Cell Death Bovinae |
| description |
The prevalent pathogens associated with bovine uterine infections are bacteria that appear to increase the host’s susceptibility to secondary infections with other bacteria or viruses, among which BoGHV4 is the most frequently found. In this work, the study of the pathways of apoptosis induction was carried out on an experimental model of primary culture of endometrial cells, in order to know the implication of BoGHV4 and the presence of bacterial LPS in the pathogenesis of the bovine reproductive tract. For this, different staining techniques and molecular analysis by RT-PCR were used. The results obtained allowed us to conclude that the level of cell death observed in the proposed primary culture is directly related to the time of viral infection and the presence of LPS in BoGHV4 infection. The apoptosis indices in cells infected with BoGHV4 and BoGHV4 + LPS revealed a maximum that correlated with the appearance of cytopathic effects and the maximum viral titers in the model studied. However, morphological, biochemical, and molecular changes were evident during both early and late stages of apoptosis. These findings provide information on the factors that may influence the pathogenesis of BoGHV4 and help to better understand the mechanisms involved in virus infection. |
| publishDate |
2024 |
| dc.date.none.fl_str_mv |
2024-04-12T11:24:21Z 2024-04-12T11:24:21Z 2024-04-08 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
http://hdl.handle.net/20.500.12123/17390 https://www.mdpi.com/2079-7737/13/4/249 2079-7737 https://doi.org/10.3390/biology13040249 |
| url |
http://hdl.handle.net/20.500.12123/17390 https://www.mdpi.com/2079-7737/13/4/249 https://doi.org/10.3390/biology13040249 |
| identifier_str_mv |
2079-7737 |
| dc.language.none.fl_str_mv |
eng |
| language |
eng |
| dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
| eu_rights_str_mv |
openAccess |
| rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
| dc.format.none.fl_str_mv |
application/pdf |
| dc.publisher.none.fl_str_mv |
MDPI |
| publisher.none.fl_str_mv |
MDPI |
| dc.source.none.fl_str_mv |
Biology 13 (4) : 249 (April 2024) reponame:INTA Digital (INTA) instname:Instituto Nacional de Tecnología Agropecuaria |
| instname_str |
Instituto Nacional de Tecnología Agropecuaria |
| reponame_str |
INTA Digital (INTA) |
| collection |
INTA Digital (INTA) |
| repository.name.fl_str_mv |
INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria |
| repository.mail.fl_str_mv |
tripaldi.nicolas@inta.gob.ar |
| _version_ |
1799197129476931584 |
| spelling |
Modulation of Apoptosis by Bovine Gammaherpesvirus 4 Infection in Bovine Endometrial Cells and the Possible Role of LPS in This ProcessRomeo, FlorenciaDelgado, Santiago GermánYavorsky, MarisolMartinez Cuesta, LucíaPereyra, Susana BeatrizGonzález Altamiranda, ErikaLouge Uriarte, Enrique LeopoldoPérez, SandraVerna, Andrea ElizabethHerpes Virus BovinoInfecciónMuerte CélularBovine HerpesvirusApoptosisInfectionCell DeathBovinaeThe prevalent pathogens associated with bovine uterine infections are bacteria that appear to increase the host’s susceptibility to secondary infections with other bacteria or viruses, among which BoGHV4 is the most frequently found. In this work, the study of the pathways of apoptosis induction was carried out on an experimental model of primary culture of endometrial cells, in order to know the implication of BoGHV4 and the presence of bacterial LPS in the pathogenesis of the bovine reproductive tract. For this, different staining techniques and molecular analysis by RT-PCR were used. The results obtained allowed us to conclude that the level of cell death observed in the proposed primary culture is directly related to the time of viral infection and the presence of LPS in BoGHV4 infection. The apoptosis indices in cells infected with BoGHV4 and BoGHV4 + LPS revealed a maximum that correlated with the appearance of cytopathic effects and the maximum viral titers in the model studied. However, morphological, biochemical, and molecular changes were evident during both early and late stages of apoptosis. These findings provide information on the factors that may influence the pathogenesis of BoGHV4 and help to better understand the mechanisms involved in virus infection.EEA BalcarceFil: Romeo, Florencia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina.Fil: Delgado, Santiago. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina.Fil: Yavorsky, Marisol. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina.Fil: Yavorsky, Marisol. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina.Fil: Martinez Cuesta, Lucía. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Centro de Investigación Veterinaria de Tandil; Argentina.Fil: Pereyra, Susana. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina.Fil: González Altamiranda, Erika. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina.Fil: Louge Uriarte, Enrique. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina.Fil: Pérez, Sandra. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Centro de Investigación Veterinaria de Tandil; Argentina.Fil: Verna, Andrea Elizabeth. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina.MDPI2024-04-12T11:24:21Z2024-04-12T11:24:21Z2024-04-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/17390https://www.mdpi.com/2079-7737/13/4/2492079-7737https://doi.org/10.3390/biology13040249Biology 13 (4) : 249 (April 2024)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2024-05-15T12:26:15Zoai:localhost:20.500.12123/17390instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2024-05-15 12:26:15.75INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse |
| score |
15,812429 |