The role of pH fronts in tissue electroporation based treatments

Treatments based on electroporation (EP) induce the formation of pores in cell membranes due to the application of pulsed electric fields. We present experimental evidence of the existence of pH fronts emerging from both electrodes during treatments based on tissue EP, for conditions found in many s...

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Autores: Maglietti, Felipe Horacio, Michinski, Sebastián Diego, Olaiz, Nahuel Manuel, Castro, Marcelo Adrian, Suárez, Cecilia Ana, Marshall, Guillermo Ricardo
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2013
País:Argentina
Institución:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/695
Acceso en línea:http://hdl.handle.net/11336/695
Access Level:acceso abierto
Palabra clave:electrochemotherapy
ph
electroporation
buffer
https://purl.org/becyt/ford/3
https://purl.org/becyt/ford/3.1
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spelling The role of pH fronts in tissue electroporation based treatmentsMaglietti, Felipe HoracioMichinski, Sebastián DiegoOlaiz, Nahuel ManuelCastro, Marcelo AdrianSuárez, Cecilia AnaMarshall, Guillermo Ricardoelectrochemotherapyphelectroporationbufferhttps://purl.org/becyt/ford/3https://purl.org/becyt/ford/3.1Treatments based on electroporation (EP) induce the formation of pores in cell membranes due to the application of pulsed electric fields. We present experimental evidence of the existence of pH fronts emerging from both electrodes during treatments based on tissue EP, for conditions found in many studies, and that these fronts are immediate and substantial. pH fronts are indirectly measured through the evanescence time (ET), defined as the time required for the tissue buffer to neutralize them. The ET was measured through a pH indicator imaged at a series of time intervals using a four-cluster hard fuzzy-c-means algorithm to segment pixels corresponding to the pH indicator at every frame. The ET was calculated as the time during which the number of pixels was 10% of those in the initial frame. While in EP-based treatments such as reversible (ECT) and irreversible electroporation (IRE) the ET is very short (though enough to cause minor injuries) due to electric pulse characteristics and biological buffers present in the tissue, in gene electrotransfer (GET), ET is much longer, enough to denaturate plasmids and produce cell damage. When any of the electric pulse parameters is doubled or tripled the ET grows and, remarkably, when any of the pulse parameters in GET is halved, the ET drops significantly. Reducing pH fronts has relevant implications for GET treatment efficiency, due to a substantial reduction of plasmid damage and cell loss.Fil: Maglietti, Felipe Horacio. Universidad de Buenos Aires. Facultad de Ingenieria. Departamento de Computacion. Laboratorio de Sistemas Complejos; Argentina;Fil: Michinski, Sebastián Diego. Consejo Nacional de Invest.cientif.y Tecnicas. Oficina de Coordinacion Administrativa Ciudad Universitaria. Instituto de Fisica del Plasma; Argentina; Instituto Tecnologico de Buenos Aires. Departamento de Bioingenieria; Argentina;Fil: Olaiz, Nahuel Manuel. Universidad de Buenos Aires. Facultad de Ingenieria. Departamento de Computacion. Laboratorio de Sistemas Complejos; Argentina;Fil: Castro, Marcelo Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina; Universidad Tecnologica Nacional; Argentina;Fil: Suárez, Cecilia Ana. Universidad de Buenos Aires. Facultad de Ingenieria. Departamento de Computacion. Laboratorio de Sistemas Complejos; Argentina;Fil: Marshall, Guillermo Ricardo. Universidad de Buenos Aires. Facultad de Ingenieria. Departamento de Computacion. Laboratorio de Sistemas Complejos; Argentina;Public Library Science2013-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/695Maglietti, Felipe Horacio; Michinski, Sebastián Diego; Olaiz, Nahuel Manuel; Castro, Marcelo Adrian; Suárez, Cecilia Ana; et al.;The role of pH fronts in tissue electroporation based treatments; Public Library Science; Plos One; 8; 11; 11-2013; e801671932-6203enginfo:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0080167info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T14:03:47Zoai:ri.conicet.gov.ar:11336/695instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 14:03:47.343CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv The role of pH fronts in tissue electroporation based treatments
title The role of pH fronts in tissue electroporation based treatments
spellingShingle The role of pH fronts in tissue electroporation based treatments
Maglietti, Felipe Horacio
electrochemotherapy
ph
electroporation
buffer
https://purl.org/becyt/ford/3
https://purl.org/becyt/ford/3.1
title_short The role of pH fronts in tissue electroporation based treatments
title_full The role of pH fronts in tissue electroporation based treatments
title_fullStr The role of pH fronts in tissue electroporation based treatments
title_full_unstemmed The role of pH fronts in tissue electroporation based treatments
title_sort The role of pH fronts in tissue electroporation based treatments
dc.creator.none.fl_str_mv Maglietti, Felipe Horacio
Michinski, Sebastián Diego
Olaiz, Nahuel Manuel
Castro, Marcelo Adrian
Suárez, Cecilia Ana
Marshall, Guillermo Ricardo
author Maglietti, Felipe Horacio
author_facet Maglietti, Felipe Horacio
Michinski, Sebastián Diego
Olaiz, Nahuel Manuel
Castro, Marcelo Adrian
Suárez, Cecilia Ana
Marshall, Guillermo Ricardo
author_role author
author2 Michinski, Sebastián Diego
Olaiz, Nahuel Manuel
Castro, Marcelo Adrian
Suárez, Cecilia Ana
Marshall, Guillermo Ricardo
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv electrochemotherapy
ph
electroporation
buffer
https://purl.org/becyt/ford/3
https://purl.org/becyt/ford/3.1
topic electrochemotherapy
ph
electroporation
buffer
https://purl.org/becyt/ford/3
https://purl.org/becyt/ford/3.1
description Treatments based on electroporation (EP) induce the formation of pores in cell membranes due to the application of pulsed electric fields. We present experimental evidence of the existence of pH fronts emerging from both electrodes during treatments based on tissue EP, for conditions found in many studies, and that these fronts are immediate and substantial. pH fronts are indirectly measured through the evanescence time (ET), defined as the time required for the tissue buffer to neutralize them. The ET was measured through a pH indicator imaged at a series of time intervals using a four-cluster hard fuzzy-c-means algorithm to segment pixels corresponding to the pH indicator at every frame. The ET was calculated as the time during which the number of pixels was 10% of those in the initial frame. While in EP-based treatments such as reversible (ECT) and irreversible electroporation (IRE) the ET is very short (though enough to cause minor injuries) due to electric pulse characteristics and biological buffers present in the tissue, in gene electrotransfer (GET), ET is much longer, enough to denaturate plasmids and produce cell damage. When any of the electric pulse parameters is doubled or tripled the ET grows and, remarkably, when any of the pulse parameters in GET is halved, the ET drops significantly. Reducing pH fronts has relevant implications for GET treatment efficiency, due to a substantial reduction of plasmid damage and cell loss.
publishDate 2013
dc.date.none.fl_str_mv 2013-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/695
Maglietti, Felipe Horacio; Michinski, Sebastián Diego; Olaiz, Nahuel Manuel; Castro, Marcelo Adrian; Suárez, Cecilia Ana; et al.;The role of pH fronts in tissue electroporation based treatments; Public Library Science; Plos One; 8; 11; 11-2013; e80167
1932-6203
url http://hdl.handle.net/11336/695
identifier_str_mv Maglietti, Felipe Horacio; Michinski, Sebastián Diego; Olaiz, Nahuel Manuel; Castro, Marcelo Adrian; Suárez, Cecilia Ana; et al.;The role of pH fronts in tissue electroporation based treatments; Public Library Science; Plos One; 8; 11; 11-2013; e80167
1932-6203
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0080167
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Public Library Science
publisher.none.fl_str_mv Public Library Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 15.811543

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