Antifungal activity of selected indigenous Pseudomonas and Bacillus from the soybean rhizosphere

The purpose of this study was to isolate and select indigenous soil Pseudomonas and Bacillus bacteria capable of developing multiple mechanisms of action related to the biocontrol of phytopathogenic fungi affecting soybean crops. The screening procedure consisted of antagonism tests against a panel...

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Bibliographic Details
Authors: León, Mariana, Yaryura, Pablo Marcelo, Montecchia, Marcela Susana, Hernandez, Alejandra Ines, Correa, Olga Susana, Pucheu, Norma Lucrecia, Kerber, Norma Lucia, Garcia, Augusto Fernando
Format: article
Status:Published version
Publication Date:2009
Country:Argentina
Institution:Consejo Nacional de Investigaciones Científicas y Técnicas
Repository:CONICET Digital (CONICET)
Language:English
OAI Identifier:oai:ri.conicet.gov.ar:11336/61112
Online Access:http://hdl.handle.net/11336/61112
Access Level:Open access
Keyword:BIOCONTROL
BACILLUS
PSEUDOMONAS
PYTHIUM ULTIMUM
SOYBEAN
RHIZOSPHERE
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
Description
Summary:The purpose of this study was to isolate and select indigenous soil Pseudomonas and Bacillus bacteria capable of developing multiple mechanisms of action related to the biocontrol of phytopathogenic fungi affecting soybean crops. The screening procedure consisted of antagonism tests against a panel of phytopathogenic fungi, taxonomic identification, detection by PCR of several genes related to antifungal activity, in vitro detection of the antifungal products, and root colonization assays. Two isolates, identified and designated as Pseudomonas fluorescens BNM296 and Bacillus amyloliquefaciens BNM340, were selected for further studies. These isolates protected plants against the damping-off caused by Pythium ultimum and were able to increase the seedling emergence rate after inoculation of soybean seeds with each bacterium. Also, the shoot nitrogen content was higher in plants when seeds were inoculated with BNM296. The polyphasic approach of this work allowed us to select two indigenous bacterial strains that promoted the early development of soybean plants.

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