Photodynamic inactivation of red cell uroporphyrinogen decarboxylase by porphyrins
1. 1. The effects of light and porphyrins on the activity of red cell uroporphyrinogen decarboxylase were studied. 2. 2. Photoinactivation of uroporphyrinogen decarboxylase was dependent on uroporphyrin concentration, irradiation time and temperature. Using 40 W/m2 of UV light intensity, 40-45% decr...
| Autores: | , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 1986 |
| País: | Argentina |
| Institución: | Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales |
| Repositorio: | Biblioteca Digital (UBA-FCEN) |
| Idioma: | inglés |
| OAI Identifier: | paperaa:paper_0020711X_v18_n2_p143_DelCBatlle |
| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_0020711X_v18_n2_p143_DelCBatlle |
| Access Level: | acceso abierto |
| Palabra clave: | porphyrin uroporphyrinogen decarboxylase blood and hemopoietic system enzyme inactivation erythrocyte human human cell photoactivation Carboxy-Lyases Erythrocytes Humans Kinetics Photolysis Porphyrins Thermodynamics Ultraviolet Rays Uroporphyrinogen Decarboxylase |
| Sumario: | 1. 1. The effects of light and porphyrins on the activity of red cell uroporphyrinogen decarboxylase were studied. 2. 2. Photoinactivation of uroporphyrinogen decarboxylase was dependent on uroporphyrin concentration, irradiation time and temperature. Using 40 W/m2 of UV light intensity, 40-45% decreased activity was produced with 200 μM uroporphyrin I, at 37°C and after 2 hr of illumination. 3. 3. It has been demonstrated that porphyrins photoinactivate uroporphyrinogen decarboxylase and a mechanism for this action in relation to skin lesions is proposed. © 1986. |
|---|