Sequestration of 9-Hydroxystearic Acid in FAHFA (Fatty Acid Esters of Hydroxy Fatty Acids) as a Protective Mechanism for Colon Carcinoma Cells to Avoid Apoptotic Cell Death

Hydroxy fatty acids are known to cause cell cycle arrest and apoptosis. The best studied of them, 9-hydroxystearic acid (9-HSA), induces apoptosis in cell lines by acting through mechanisms involving different targets. Using mass spectrometry-based lipidomic approaches, we show in this study that 9-...

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Detalhes bibliográficos
Autores: Rodríguez, Juan Pablo, Guijas, Carlos, Astudillo, Alma M., Rubio, Julio M., Balboa, María A., Balsinde Rodríguez, Jesús
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2019
País:Argentina
Recursos:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/108583
Acesso em linha:http://hdl.handle.net/11336/108583
Access Level:acceso abierto
Palavra-chave:APOPTOSIS
COLORECTAL CANCER
FATTY ACID ESTERS OF HYDROXY FATTY ACIDS
HYDROXYSTEARIC ACID
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
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network_acronym_str AR
network_name_str Argentina
repository_id_str
dc.title.none.fl_str_mv Sequestration of 9-Hydroxystearic Acid in FAHFA (Fatty Acid Esters of Hydroxy Fatty Acids) as a Protective Mechanism for Colon Carcinoma Cells to Avoid Apoptotic Cell Death
title Sequestration of 9-Hydroxystearic Acid in FAHFA (Fatty Acid Esters of Hydroxy Fatty Acids) as a Protective Mechanism for Colon Carcinoma Cells to Avoid Apoptotic Cell Death
spellingShingle Sequestration of 9-Hydroxystearic Acid in FAHFA (Fatty Acid Esters of Hydroxy Fatty Acids) as a Protective Mechanism for Colon Carcinoma Cells to Avoid Apoptotic Cell Death
Rodríguez, Juan Pablo
APOPTOSIS
COLORECTAL CANCER
FATTY ACID ESTERS OF HYDROXY FATTY ACIDS
HYDROXYSTEARIC ACID
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
title_short Sequestration of 9-Hydroxystearic Acid in FAHFA (Fatty Acid Esters of Hydroxy Fatty Acids) as a Protective Mechanism for Colon Carcinoma Cells to Avoid Apoptotic Cell Death
title_full Sequestration of 9-Hydroxystearic Acid in FAHFA (Fatty Acid Esters of Hydroxy Fatty Acids) as a Protective Mechanism for Colon Carcinoma Cells to Avoid Apoptotic Cell Death
title_fullStr Sequestration of 9-Hydroxystearic Acid in FAHFA (Fatty Acid Esters of Hydroxy Fatty Acids) as a Protective Mechanism for Colon Carcinoma Cells to Avoid Apoptotic Cell Death
title_full_unstemmed Sequestration of 9-Hydroxystearic Acid in FAHFA (Fatty Acid Esters of Hydroxy Fatty Acids) as a Protective Mechanism for Colon Carcinoma Cells to Avoid Apoptotic Cell Death
title_sort Sequestration of 9-Hydroxystearic Acid in FAHFA (Fatty Acid Esters of Hydroxy Fatty Acids) as a Protective Mechanism for Colon Carcinoma Cells to Avoid Apoptotic Cell Death
dc.creator.none.fl_str_mv Rodríguez, Juan Pablo
Guijas, Carlos
Astudillo, Alma M.
Rubio, Julio M.
Balboa, María A.
Balsinde Rodríguez, Jesús
author Rodríguez, Juan Pablo
author_facet Rodríguez, Juan Pablo
Guijas, Carlos
Astudillo, Alma M.
Rubio, Julio M.
Balboa, María A.
Balsinde Rodríguez, Jesús
author_role author
author2 Guijas, Carlos
Astudillo, Alma M.
Rubio, Julio M.
Balboa, María A.
Balsinde Rodríguez, Jesús
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv APOPTOSIS
COLORECTAL CANCER
FATTY ACID ESTERS OF HYDROXY FATTY ACIDS
HYDROXYSTEARIC ACID
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
topic APOPTOSIS
COLORECTAL CANCER
FATTY ACID ESTERS OF HYDROXY FATTY ACIDS
HYDROXYSTEARIC ACID
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
description Hydroxy fatty acids are known to cause cell cycle arrest and apoptosis. The best studied of them, 9-hydroxystearic acid (9-HSA), induces apoptosis in cell lines by acting through mechanisms involving different targets. Using mass spectrometry-based lipidomic approaches, we show in this study that 9-HSA levels in human colorectal tumors are diminished when compared with normal adjacent tissue. Since this decrease could be compatible with an escape mechanism of tumors from 9-HSA-induced apoptosis, we investigated different features of the utilization of this hydroxyfatty acid in colon. We show that in colorectal tumors and related cell lines such as HT-29 and HCT-116, 9-HSA is the only hydroxyfatty acid constituent of branched fatty acid esters of hydroxyfatty acids (FAHFA), a novel family of lipids with anti-inflammatory properties. Importantly, FAHFA levels in tumors are elevated compared with normal tissue and, unlike 9-HSA, they do not induce apoptosis of colorectal cell lines over a wide range of concentrations. Further, the addition of 9-HSA to colon cancer cell lines augments the synthesis of different FAHFA before the cells commit to apoptosis, suggesting that FAHFA formation may function as a buffer system that sequesters the hydroxyacid into an inactive form, thereby restricting apoptosis.
publishDate 2019
dc.date.none.fl_str_mv 2019-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/108583
Rodríguez, Juan Pablo; Guijas, Carlos; Astudillo, Alma M.; Rubio, Julio M.; Balboa, María A.; et al.; Sequestration of 9-Hydroxystearic Acid in FAHFA (Fatty Acid Esters of Hydroxy Fatty Acids) as a Protective Mechanism for Colon Carcinoma Cells to Avoid Apoptotic Cell Death; MDPI; Cancers; 11; 4; 4-2019
2072-6694
CONICET Digital
CONICET
url http://hdl.handle.net/11336/108583
identifier_str_mv Rodríguez, Juan Pablo; Guijas, Carlos; Astudillo, Alma M.; Rubio, Julio M.; Balboa, María A.; et al.; Sequestration of 9-Hydroxystearic Acid in FAHFA (Fatty Acid Esters of Hydroxy Fatty Acids) as a Protective Mechanism for Colon Carcinoma Cells to Avoid Apoptotic Cell Death; MDPI; Cancers; 11; 4; 4-2019
2072-6694
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2072-6694/11/4/524
info:eu-repo/semantics/altIdentifier/doi/10.3390/cancers11040524
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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spelling Sequestration of 9-Hydroxystearic Acid in FAHFA (Fatty Acid Esters of Hydroxy Fatty Acids) as a Protective Mechanism for Colon Carcinoma Cells to Avoid Apoptotic Cell DeathRodríguez, Juan PabloGuijas, CarlosAstudillo, Alma M.Rubio, Julio M.Balboa, María A.Balsinde Rodríguez, JesúsAPOPTOSISCOLORECTAL CANCERFATTY ACID ESTERS OF HYDROXY FATTY ACIDSHYDROXYSTEARIC ACIDhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Hydroxy fatty acids are known to cause cell cycle arrest and apoptosis. The best studied of them, 9-hydroxystearic acid (9-HSA), induces apoptosis in cell lines by acting through mechanisms involving different targets. Using mass spectrometry-based lipidomic approaches, we show in this study that 9-HSA levels in human colorectal tumors are diminished when compared with normal adjacent tissue. Since this decrease could be compatible with an escape mechanism of tumors from 9-HSA-induced apoptosis, we investigated different features of the utilization of this hydroxyfatty acid in colon. We show that in colorectal tumors and related cell lines such as HT-29 and HCT-116, 9-HSA is the only hydroxyfatty acid constituent of branched fatty acid esters of hydroxyfatty acids (FAHFA), a novel family of lipids with anti-inflammatory properties. Importantly, FAHFA levels in tumors are elevated compared with normal tissue and, unlike 9-HSA, they do not induce apoptosis of colorectal cell lines over a wide range of concentrations. Further, the addition of 9-HSA to colon cancer cell lines augments the synthesis of different FAHFA before the cells commit to apoptosis, suggesting that FAHFA formation may function as a buffer system that sequesters the hydroxyacid into an inactive form, thereby restricting apoptosis.Fil: Rodríguez, Juan Pablo. Consejo Superior de Investigaciones Científicas; España. Universidad de Valladolid; España. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Química Básica y Aplicada del Nordeste Argentino. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Instituto de Química Básica y Aplicada del Nordeste Argentino; ArgentinaFil: Guijas, Carlos. Centro de Investigación Biomédica en Red de Diabetes y Enfermedades Metabólicas Asociadas; España. Universidad de Valladolid; EspañaFil: Astudillo, Alma M.. Centro de Investigación Biomédica en Red de Diabetes y Enfermedades Metabólicas Asociadas; España. Universidad de Valladolid; EspañaFil: Rubio, Julio M.. Centro de Investigación Biomédica en Red de Diabetes y Enfermedades Metabólicas Asociadas; España. Universidad de Valladolid; EspañaFil: Balboa, María A.. Centro de Investigación Biomédica en Red de Diabetes y Enfermedades Metabólicas Asociadas; España. Universidad de Valladolid; EspañaFil: Balsinde Rodríguez, Jesús. Centro de Investigación Biomédica en Red de Diabetes y Enfermedades Metabólicas Asociadas; España. Universidad de Valladolid; EspañaMDPI2019-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/108583Rodríguez, Juan Pablo; Guijas, Carlos; Astudillo, Alma M.; Rubio, Julio M.; Balboa, María A.; et al.; Sequestration of 9-Hydroxystearic Acid in FAHFA (Fatty Acid Esters of Hydroxy Fatty Acids) as a Protective Mechanism for Colon Carcinoma Cells to Avoid Apoptotic Cell Death; MDPI; Cancers; 11; 4; 4-20192072-6694CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2072-6694/11/4/524info:eu-repo/semantics/altIdentifier/doi/10.3390/cancers11040524info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T14:17:11Zoai:ri.conicet.gov.ar:11336/108583instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 14:17:12.178CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
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