Effect of soil chemical fertilization on the diversity and composition of the tomato endophytic diazotrophic community at different stages of growth

The aim of this work was to gain a more comprehensive and perspicacious view of the endophytic diazotrophic community (EDC) of tomato plant bacteria and assess the effects of chemical fertilization and the plant phenologic stage on the status of those microbes. When the EDC of stem and roots from to...

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Detalles Bibliográficos
Autores: Collavino, Mónica Mariana, Ramos Cabrera, Efren Venancio, Bruno, Cecilia Ines, Aguilar, Orlando Mario
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2020
País:Argentina
Institución:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/146046
Acceso en línea:http://hdl.handle.net/11336/146046
Access Level:acceso abierto
Palabra clave:ENDOPHYTES
N2-FIXING BACTERIA
NIFH GENE PYROSEQUENCING
TOMATO
https://purl.org/becyt/ford/4.4
https://purl.org/becyt/ford/4
id AR_a5cb4e2fe5f467f25bfb8ecbdd37cf32
oai_identifier_str oai:ri.conicet.gov.ar:11336/146046
network_acronym_str AR
network_name_str Argentina
repository_id_str
dc.title.none.fl_str_mv Effect of soil chemical fertilization on the diversity and composition of the tomato endophytic diazotrophic community at different stages of growth
title Effect of soil chemical fertilization on the diversity and composition of the tomato endophytic diazotrophic community at different stages of growth
spellingShingle Effect of soil chemical fertilization on the diversity and composition of the tomato endophytic diazotrophic community at different stages of growth
Collavino, Mónica Mariana
ENDOPHYTES
N2-FIXING BACTERIA
NIFH GENE PYROSEQUENCING
TOMATO
https://purl.org/becyt/ford/4.4
https://purl.org/becyt/ford/4
title_short Effect of soil chemical fertilization on the diversity and composition of the tomato endophytic diazotrophic community at different stages of growth
title_full Effect of soil chemical fertilization on the diversity and composition of the tomato endophytic diazotrophic community at different stages of growth
title_fullStr Effect of soil chemical fertilization on the diversity and composition of the tomato endophytic diazotrophic community at different stages of growth
title_full_unstemmed Effect of soil chemical fertilization on the diversity and composition of the tomato endophytic diazotrophic community at different stages of growth
title_sort Effect of soil chemical fertilization on the diversity and composition of the tomato endophytic diazotrophic community at different stages of growth
dc.creator.none.fl_str_mv Collavino, Mónica Mariana
Ramos Cabrera, Efren Venancio
Bruno, Cecilia Ines
Aguilar, Orlando Mario
author Collavino, Mónica Mariana
author_facet Collavino, Mónica Mariana
Ramos Cabrera, Efren Venancio
Bruno, Cecilia Ines
Aguilar, Orlando Mario
author_role author
author2 Ramos Cabrera, Efren Venancio
Bruno, Cecilia Ines
Aguilar, Orlando Mario
author2_role author
author
author
dc.subject.none.fl_str_mv ENDOPHYTES
N2-FIXING BACTERIA
NIFH GENE PYROSEQUENCING
TOMATO
https://purl.org/becyt/ford/4.4
https://purl.org/becyt/ford/4
topic ENDOPHYTES
N2-FIXING BACTERIA
NIFH GENE PYROSEQUENCING
TOMATO
https://purl.org/becyt/ford/4.4
https://purl.org/becyt/ford/4
description The aim of this work was to gain a more comprehensive and perspicacious view of the endophytic diazotrophic community (EDC) of tomato plant bacteria and assess the effects of chemical fertilization and the plant phenologic stage on the status of those microbes. When the EDC of stem and roots from tomato plants grown in a greenhouse with and without exogenous chemical fertilization was examined by pyrosequencing the nifH gene during the growth cycle, a high taxonomic and phylogenetic diversity was observed. The abundant taxa were related to ubiquitous endophytes such as Rhizobium or Burkholderia but also involved anaerobic members usually restricted to flooded plant tissues, such as Clostridium, Geobacter, and Desulfovibrio. The EDC composition appeared to be dynamic during the growth phase of the tomato, with the structure of the community at the early stages of growth displaying major differences from the late stages. Inorganic fertilization negatively affected the diversity and modified the profile of the predominant components of the EDC in the different growth stages. Populations such as Burkholderia and Geobacter plus the Cyanobacteria appeared particularly affected by fertilization. Our work demonstrates an extensive endophytic diazotrophic diversity, suggesting a high potential for nitrogen fixation. The effect of the phenologic stage and inorganic-chemical soil fertilization on the community structure indicated a dynamic community that responded to environmental changes. These findings contribute to a better understanding of endophytic associations that could be helpful in assisting to shape the endomicrobiome that provides essential benefits to crops.
publishDate 2020
dc.date.none.fl_str_mv 2020-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/146046
Collavino, Mónica Mariana; Ramos Cabrera, Efren Venancio; Bruno, Cecilia Ines; Aguilar, Orlando Mario; Effect of soil chemical fertilization on the diversity and composition of the tomato endophytic diazotrophic community at different stages of growth; Springer; Brazilian Journal of Microbiology; 51; 4; 12-2020; 1965-1975
1517-8382
1678-4405
CONICET Digital
CONICET
url http://hdl.handle.net/11336/146046
identifier_str_mv Collavino, Mónica Mariana; Ramos Cabrera, Efren Venancio; Bruno, Cecilia Ines; Aguilar, Orlando Mario; Effect of soil chemical fertilization on the diversity and composition of the tomato endophytic diazotrophic community at different stages of growth; Springer; Brazilian Journal of Microbiology; 51; 4; 12-2020; 1965-1975
1517-8382
1678-4405
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1007/s42770-020-00373-3
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs42770-020-00373-3
info:eu-repo/semantics/altIdentifier/url/https://europepmc.org/article/med/32895888#free-full-text
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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spelling Effect of soil chemical fertilization on the diversity and composition of the tomato endophytic diazotrophic community at different stages of growthCollavino, Mónica MarianaRamos Cabrera, Efren VenancioBruno, Cecilia InesAguilar, Orlando MarioENDOPHYTESN2-FIXING BACTERIANIFH GENE PYROSEQUENCINGTOMATOhttps://purl.org/becyt/ford/4.4https://purl.org/becyt/ford/4The aim of this work was to gain a more comprehensive and perspicacious view of the endophytic diazotrophic community (EDC) of tomato plant bacteria and assess the effects of chemical fertilization and the plant phenologic stage on the status of those microbes. When the EDC of stem and roots from tomato plants grown in a greenhouse with and without exogenous chemical fertilization was examined by pyrosequencing the nifH gene during the growth cycle, a high taxonomic and phylogenetic diversity was observed. The abundant taxa were related to ubiquitous endophytes such as Rhizobium or Burkholderia but also involved anaerobic members usually restricted to flooded plant tissues, such as Clostridium, Geobacter, and Desulfovibrio. The EDC composition appeared to be dynamic during the growth phase of the tomato, with the structure of the community at the early stages of growth displaying major differences from the late stages. Inorganic fertilization negatively affected the diversity and modified the profile of the predominant components of the EDC in the different growth stages. Populations such as Burkholderia and Geobacter plus the Cyanobacteria appeared particularly affected by fertilization. Our work demonstrates an extensive endophytic diazotrophic diversity, suggesting a high potential for nitrogen fixation. The effect of the phenologic stage and inorganic-chemical soil fertilization on the community structure indicated a dynamic community that responded to environmental changes. These findings contribute to a better understanding of endophytic associations that could be helpful in assisting to shape the endomicrobiome that provides essential benefits to crops.Fil: Collavino, Mónica Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; ArgentinaFil: Ramos Cabrera, Efren Venancio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Bruno, Cecilia Ines. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigaciones Agropecuarias. Unidad de Fitopatologia y Modelizacion Agricola. Grupo Vinculado Catedra de Estadistica y Biometria de la Facultad de Ciencias Agropecuarias de la Universidad Nacional de Cordoba Al Ufyma | Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Unidad de Fitopatologia y Modelizacion Agricola. Grupo Vinculado Catedra de Estadistica y Biometria de la Facultad de Ciencias Agropecuarias de la Universidad Nacional de Cordoba Al Ufyma.; ArgentinaFil: Aguilar, Orlando Mario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaSpringer2020-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/146046Collavino, Mónica Mariana; Ramos Cabrera, Efren Venancio; Bruno, Cecilia Ines; Aguilar, Orlando Mario; Effect of soil chemical fertilization on the diversity and composition of the tomato endophytic diazotrophic community at different stages of growth; Springer; Brazilian Journal of Microbiology; 51; 4; 12-2020; 1965-19751517-83821678-4405CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1007/s42770-020-00373-3info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs42770-020-00373-3info:eu-repo/semantics/altIdentifier/url/https://europepmc.org/article/med/32895888#free-full-textinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T13:56:54Zoai:ri.conicet.gov.ar:11336/146046instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 13:56:55.152CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
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