Lipopolysaccharide impairs hepatocyte ureagenesis from ammonia: Involvement of mitochondrial aquaporin-8

We recently reported that hepatocyte mitochondrial aquaporin-8 (mtAQP8) channels facilitate the uptake of ammonia and its metabolism into urea. Here we studied the effect of bacterial lipopolysaccharides (LPS) on ammonia-derived ureagenesis. In LPS-treated rats, hepatic mtAQP8 protein expression and...

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Detalhes bibliográficos
Autores: Soria, Leandro Raul, Marrone, Julieta, Molinas, Sara Maria, Lehmann Mantaras, Guillermo Luis, Calamita, Giuseppe, Marinelli, Raul Alberto
Tipo de documento: artigo
Estado:Versão publicada
Data de publicação:2014
País:Argentina
Recursos:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositório:CONICET Digital (CONICET)
Idioma:inglês
OAI Identifier:oai:ri.conicet.gov.ar:11336/6032
Acesso em linha:http://hdl.handle.net/11336/6032
Access Level:Acceso aberto
Palavra-chave:Mitochondria
Aquaporin-8
Ammonia Transpot
Liver
https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
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repository_id_str
spelling Lipopolysaccharide impairs hepatocyte ureagenesis from ammonia: Involvement of mitochondrial aquaporin-8Soria, Leandro RaulMarrone, JulietaMolinas, Sara MariaLehmann Mantaras, Guillermo LuisCalamita, GiuseppeMarinelli, Raul AlbertoMitochondriaAquaporin-8Ammonia TranspotLiverhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3We recently reported that hepatocyte mitochondrial aquaporin-8 (mtAQP8) channels facilitate the uptake of ammonia and its metabolism into urea. Here we studied the effect of bacterial lipopolysaccharides (LPS) on ammonia-derived ureagenesis. In LPS-treated rats, hepatic mtAQP8 protein expression and diffusional ammonia permeability (measured utilizing ammonia analogues) of liver inner mitochondrial membranes were downregulated. NMR studies using 15N-labeled ammonia indicated that basal and glucagon-induced ureagenesis from ammonia were significantly reduced in hepatocytes from LPS-treated rats. Our data suggest that hepatocyte mtAQP8-mediated ammonia removal via ureagenesis is impaired by LPS, a mechanism potentially relevant to the molecular pathogenesis of defective hepatic ammonia detoxification in sepsis.Fil: Soria, Leandro Raul. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Fisiología Experimental (i); ArgentinaFil: Marrone, Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Fisiología Experimental (i); ArgentinaFil: Molinas, Sara Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Fisiología Experimental (i); ArgentinaFil: Lehmann Mantaras, Guillermo Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Fisiología Experimental (i); Argentina. Weill Cornell Medical College. Margaret Dyson Vision Research Institute; Estados UnidosFil: Calamita, Giuseppe. Universita Degli Studi Di Bari; ItaliaFil: Marinelli, Raul Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Fisiología Experimental (i); ArgentinaElsevier2014-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/zipapplication/pdfhttp://hdl.handle.net/11336/6032Soria, Leandro Raul; Marrone, Julieta; Molinas, Sara Maria; Lehmann Mantaras, Guillermo Luis; Calamita, Giuseppe; et al.; Lipopolysaccharide impairs hepatocyte ureagenesis from ammonia: Involvement of mitochondrial aquaporin-8; Elsevier; FEBS Letters; 588; 9; 3-2014; 1686-16910014-5793enginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0014579314002075info:eu-repo/semantics/altIdentifier/doi/10.1016/j.febslet.2014.03.012info:eu-repo/semantics/altIdentifier/doi/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T14:22:17Zoai:ri.conicet.gov.ar:11336/6032instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 14:22:17.669CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Lipopolysaccharide impairs hepatocyte ureagenesis from ammonia: Involvement of mitochondrial aquaporin-8
title Lipopolysaccharide impairs hepatocyte ureagenesis from ammonia: Involvement of mitochondrial aquaporin-8
spellingShingle Lipopolysaccharide impairs hepatocyte ureagenesis from ammonia: Involvement of mitochondrial aquaporin-8
Soria, Leandro Raul
Mitochondria
Aquaporin-8
Ammonia Transpot
Liver
https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
title_short Lipopolysaccharide impairs hepatocyte ureagenesis from ammonia: Involvement of mitochondrial aquaporin-8
title_full Lipopolysaccharide impairs hepatocyte ureagenesis from ammonia: Involvement of mitochondrial aquaporin-8
title_fullStr Lipopolysaccharide impairs hepatocyte ureagenesis from ammonia: Involvement of mitochondrial aquaporin-8
title_full_unstemmed Lipopolysaccharide impairs hepatocyte ureagenesis from ammonia: Involvement of mitochondrial aquaporin-8
title_sort Lipopolysaccharide impairs hepatocyte ureagenesis from ammonia: Involvement of mitochondrial aquaporin-8
dc.creator.none.fl_str_mv Soria, Leandro Raul
Marrone, Julieta
Molinas, Sara Maria
Lehmann Mantaras, Guillermo Luis
Calamita, Giuseppe
Marinelli, Raul Alberto
author Soria, Leandro Raul
author_facet Soria, Leandro Raul
Marrone, Julieta
Molinas, Sara Maria
Lehmann Mantaras, Guillermo Luis
Calamita, Giuseppe
Marinelli, Raul Alberto
author_role author
author2 Marrone, Julieta
Molinas, Sara Maria
Lehmann Mantaras, Guillermo Luis
Calamita, Giuseppe
Marinelli, Raul Alberto
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Mitochondria
Aquaporin-8
Ammonia Transpot
Liver
https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
topic Mitochondria
Aquaporin-8
Ammonia Transpot
Liver
https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
description We recently reported that hepatocyte mitochondrial aquaporin-8 (mtAQP8) channels facilitate the uptake of ammonia and its metabolism into urea. Here we studied the effect of bacterial lipopolysaccharides (LPS) on ammonia-derived ureagenesis. In LPS-treated rats, hepatic mtAQP8 protein expression and diffusional ammonia permeability (measured utilizing ammonia analogues) of liver inner mitochondrial membranes were downregulated. NMR studies using 15N-labeled ammonia indicated that basal and glucagon-induced ureagenesis from ammonia were significantly reduced in hepatocytes from LPS-treated rats. Our data suggest that hepatocyte mtAQP8-mediated ammonia removal via ureagenesis is impaired by LPS, a mechanism potentially relevant to the molecular pathogenesis of defective hepatic ammonia detoxification in sepsis.
publishDate 2014
dc.date.none.fl_str_mv 2014-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/6032
Soria, Leandro Raul; Marrone, Julieta; Molinas, Sara Maria; Lehmann Mantaras, Guillermo Luis; Calamita, Giuseppe; et al.; Lipopolysaccharide impairs hepatocyte ureagenesis from ammonia: Involvement of mitochondrial aquaporin-8; Elsevier; FEBS Letters; 588; 9; 3-2014; 1686-1691
0014-5793
url http://hdl.handle.net/11336/6032
identifier_str_mv Soria, Leandro Raul; Marrone, Julieta; Molinas, Sara Maria; Lehmann Mantaras, Guillermo Luis; Calamita, Giuseppe; et al.; Lipopolysaccharide impairs hepatocyte ureagenesis from ammonia: Involvement of mitochondrial aquaporin-8; Elsevier; FEBS Letters; 588; 9; 3-2014; 1686-1691
0014-5793
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0014579314002075
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.febslet.2014.03.012
info:eu-repo/semantics/altIdentifier/doi/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/zip
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 15,198674

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