S-nitrosylation of NF-κB p65 inhibits TSH-induced Na+/I- symporter expression
Nitric oxide (NO) is a ubiquitous signaling molecule involved in a wide variety of cellular physiological processes. In thyroid cells, NO-synthase III-endogenously produced NO reduces TSH-stimulatedthyroid-specificgene expression, suggesting a potential autocrine role of NO in modulating thyroid fun...
| Autores: | , , , , , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2015 |
| País: | Argentina |
| Institución: | Consejo Nacional de Investigaciones Científicas y Técnicas |
| Repositorio: | CONICET Digital (CONICET) |
| Idioma: | inglés |
| OAI Identifier: | oai:ri.conicet.gov.ar:11336/185963 |
| Acceso en línea: | http://hdl.handle.net/11336/185963 |
| Access Level: | acceso abierto |
| Palabra clave: | S-NITROSYLATION NFKB NIS https://purl.org/becyt/ford/3.1 https://purl.org/becyt/ford/3 |
| Sumario: | Nitric oxide (NO) is a ubiquitous signaling molecule involved in a wide variety of cellular physiological processes. In thyroid cells, NO-synthase III-endogenously produced NO reduces TSH-stimulatedthyroid-specificgene expression, suggesting a potential autocrine role of NO in modulating thyroid function. Further studies indicate that NO induces thyroid dedifferentiation, because NO donors repress TSH-stimulated iodide (I-) uptake. Here, we investigated the molecular mechanism underlying the NO-inhibited Na+/I- symporter (NIS)-mediated I- uptake in thyroid cells. We showed that NO donors reduce I- uptake in a concentration-dependent manner, which correlates with decreased NIS protein expression. NO-reduced I- uptake results from transcriptional repression of NIS gene ratherthan posttranslational modifications reducing functional NIS expression at the plasma membrane. We observed that NO donors repress TSH-induced NIS gene expression by reducing the transcriptional activity of the nuclear factor-κB subunit p65. NO-promoted p65 S-nitrosylation reduces p65-mediated transactivation of the NIS promoter in response to TSH stimulation. Overall, our data are consistent with the notion that NO plays a role as an inhibitory signal to counterbalance TSH-stimulated nuclear factor-κB activation, thus modulating thyroid hormone biosynthesis. |
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