Determination of mercury in vegetal tissues by microPIXE: Application to the study of hyperaccumulation by Spirodela intermedia (Lemnaceae)

Background and aims: Aqueous mercury (II), Hg2+, is still nowadays a hazardous pollutant with a large dispersion. Phytoremediation strategies are an environmental friendly and low-cost alternative. In order to improve these processes, Spirodela intermedia, an autochthonous floating macrophyte, was u...

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Autores: De La Fournière, Emmanuel M., Vega, Nahuel Agustín, Müller, Nahuel Agustín, Pizarro, Ramón Augusto, Debray, Mario Ernesto
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2019
País:Argentina
Recursos:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/122932
Acesso em linha:http://hdl.handle.net/11336/122932
Access Level:acceso abierto
Palavra-chave:HYPERACCUMULATION
MERCURY
MICROPIXE
SPIRODELA INTERMEDIA
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
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network_acronym_str AR
network_name_str Argentina
repository_id_str
dc.title.none.fl_str_mv Determination of mercury in vegetal tissues by microPIXE: Application to the study of hyperaccumulation by Spirodela intermedia (Lemnaceae)
Determinación del mercurio en tejidos vegetales por microPIXE: Aplicación al estudio de la hiperacumulación por Spirodela intermedia (Lemnaceae)
title Determination of mercury in vegetal tissues by microPIXE: Application to the study of hyperaccumulation by Spirodela intermedia (Lemnaceae)
spellingShingle Determination of mercury in vegetal tissues by microPIXE: Application to the study of hyperaccumulation by Spirodela intermedia (Lemnaceae)
De La Fournière, Emmanuel M.
HYPERACCUMULATION
MERCURY
MICROPIXE
SPIRODELA INTERMEDIA
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
title_short Determination of mercury in vegetal tissues by microPIXE: Application to the study of hyperaccumulation by Spirodela intermedia (Lemnaceae)
title_full Determination of mercury in vegetal tissues by microPIXE: Application to the study of hyperaccumulation by Spirodela intermedia (Lemnaceae)
title_fullStr Determination of mercury in vegetal tissues by microPIXE: Application to the study of hyperaccumulation by Spirodela intermedia (Lemnaceae)
title_full_unstemmed Determination of mercury in vegetal tissues by microPIXE: Application to the study of hyperaccumulation by Spirodela intermedia (Lemnaceae)
title_sort Determination of mercury in vegetal tissues by microPIXE: Application to the study of hyperaccumulation by Spirodela intermedia (Lemnaceae)
dc.creator.none.fl_str_mv De La Fournière, Emmanuel M.
Vega, Nahuel Agustín
Müller, Nahuel Agustín
Pizarro, Ramón Augusto
Debray, Mario Ernesto
author De La Fournière, Emmanuel M.
author_facet De La Fournière, Emmanuel M.
Vega, Nahuel Agustín
Müller, Nahuel Agustín
Pizarro, Ramón Augusto
Debray, Mario Ernesto
author_role author
author2 Vega, Nahuel Agustín
Müller, Nahuel Agustín
Pizarro, Ramón Augusto
Debray, Mario Ernesto
author2_role author
author
author
author
dc.subject.none.fl_str_mv HYPERACCUMULATION
MERCURY
MICROPIXE
SPIRODELA INTERMEDIA
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
topic HYPERACCUMULATION
MERCURY
MICROPIXE
SPIRODELA INTERMEDIA
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
description Background and aims: Aqueous mercury (II), Hg2+, is still nowadays a hazardous pollutant with a large dispersion. Phytoremediation strategies are an environmental friendly and low-cost alternative. In order to improve these processes, Spirodela intermedia, an autochthonous floating macrophyte, was used to remove Hg2+ from mineral water under laboratory conditions, studying the in vivo distribution of mercury and other elements by nuclear microprobe scanning mapping. M&M: Exposures (1 and 10 mg.L-1 Hg2+ concentrations) were performed during at least 2 weeks. All the parameters from the bioremediation process as uptake rate, bioconcentration factors (BCFs) of mercury in roots and leaves and translocation factors (TFs), were achieved from microPIXE quantifications at Buenos Aires Tandar accelerator. Results: For 1 and 10 mg.L-1 concentrations, S. intermedia can be considered as a hyperaccumulator. The highest BCFs (> 1000 in roots and > 200 in leaves) were obtained for 1 mg.L-1 of Hg2+ at 96 h. In all cases TFs < 1 were measured, indicating that Hg2+ translocation is not taking place. High resolution spatial 2D maps of the in vivo distribution for different exposure conditions were established. It was observed that Hg2+ distribution in leaves is more heterogeneous than in roots. An important finding was the detection of Hg in chlorenchyma where its effects are more toxic. Correlation between mercury and calcium distribution and its relationship with physiological responses to intoxication have been examined. Conclusions: Phytoremediation of Hg2+ by S. intermedia is a convenient alternative. Since the protocol was performed using a real water, it becomes an advisable tool at higher scale.
publishDate 2019
dc.date.none.fl_str_mv 2019-06-20
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/122932
De La Fournière, Emmanuel M.; Vega, Nahuel Agustín; Müller, Nahuel Agustín; Pizarro, Ramón Augusto; Debray, Mario Ernesto; Determination of mercury in vegetal tissues by microPIXE: Application to the study of hyperaccumulation by Spirodela intermedia (Lemnaceae); Sociedad Argentina de Botánica; Boletín de la Sociedad Argentina de Botánica; 54; 2; 20-6-2019; 263-275
0373-580X
1851-2372
CONICET Digital
CONICET
url http://hdl.handle.net/11336/122932
identifier_str_mv De La Fournière, Emmanuel M.; Vega, Nahuel Agustín; Müller, Nahuel Agustín; Pizarro, Ramón Augusto; Debray, Mario Ernesto; Determination of mercury in vegetal tissues by microPIXE: Application to the study of hyperaccumulation by Spirodela intermedia (Lemnaceae); Sociedad Argentina de Botánica; Boletín de la Sociedad Argentina de Botánica; 54; 2; 20-6-2019; 263-275
0373-580X
1851-2372
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://revistas.unc.edu.ar/index.php/BSAB/article/view/24373
info:eu-repo/semantics/altIdentifier/doi/10.31055/1851.2372.v54.n2.24373
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Sociedad Argentina de Botánica
publisher.none.fl_str_mv Sociedad Argentina de Botánica
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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spelling Determination of mercury in vegetal tissues by microPIXE: Application to the study of hyperaccumulation by Spirodela intermedia (Lemnaceae)Determinación del mercurio en tejidos vegetales por microPIXE: Aplicación al estudio de la hiperacumulación por Spirodela intermedia (Lemnaceae)De La Fournière, Emmanuel M.Vega, Nahuel AgustínMüller, Nahuel AgustínPizarro, Ramón AugustoDebray, Mario ErnestoHYPERACCUMULATIONMERCURYMICROPIXESPIRODELA INTERMEDIAhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Background and aims: Aqueous mercury (II), Hg2+, is still nowadays a hazardous pollutant with a large dispersion. Phytoremediation strategies are an environmental friendly and low-cost alternative. In order to improve these processes, Spirodela intermedia, an autochthonous floating macrophyte, was used to remove Hg2+ from mineral water under laboratory conditions, studying the in vivo distribution of mercury and other elements by nuclear microprobe scanning mapping. M&M: Exposures (1 and 10 mg.L-1 Hg2+ concentrations) were performed during at least 2 weeks. All the parameters from the bioremediation process as uptake rate, bioconcentration factors (BCFs) of mercury in roots and leaves and translocation factors (TFs), were achieved from microPIXE quantifications at Buenos Aires Tandar accelerator. Results: For 1 and 10 mg.L-1 concentrations, S. intermedia can be considered as a hyperaccumulator. The highest BCFs (> 1000 in roots and > 200 in leaves) were obtained for 1 mg.L-1 of Hg2+ at 96 h. In all cases TFs < 1 were measured, indicating that Hg2+ translocation is not taking place. High resolution spatial 2D maps of the in vivo distribution for different exposure conditions were established. It was observed that Hg2+ distribution in leaves is more heterogeneous than in roots. An important finding was the detection of Hg in chlorenchyma where its effects are more toxic. Correlation between mercury and calcium distribution and its relationship with physiological responses to intoxication have been examined. Conclusions: Phytoremediation of Hg2+ by S. intermedia is a convenient alternative. Since the protocol was performed using a real water, it becomes an advisable tool at higher scale.Introducción y objetivos: El mercurio (II) acuoso, Hg2+, es todavía un contaminante peligroso ampliamente distribuido. Las estrategias de fitorremediación son ambientalmente amigables y de bajo costo. Con el fin de optimizar estos procesos, se utilizó Spirodela intermedia, una macrófita acuática autóctona, para remover Hg2+ en agua mineral, en condiciones de laboratorio, estudiando la distribución in vivo de mercurio y otros elementos por mapeo barriendo con una microsonda nuclear. M&M: Las exposiciones (concentraciones de Hg2+ de 1 y 10 mg.L-1) duraron al menos 2 semanas. Todos los parámetros del proceso como tasa de captación, factores de bioconcentración (BCFs) de mercurio en raíces y frondes y factores de translocación (TFs) fueron calculados a partir de cuantificaciones de microPIXE con el acelerador Tandar de Buenos Aires. Resultados: S. intermedia puede ser considerado un hiperacumulador. Los más altos BCFs (> 1000 en raíces y > 200 en frondes) correspondieron a 1 mg.L-1 a las 96 hs. En todos los casos, se constató que TFs < 1, indicando que no ocurre translocación de Hg2+. Se obtuvieron mapas 2D de alta resolución espacial de la distribución elemental in vivo para las diferentes condiciones. Se observó que la distribución de mercurio en frondes es más heterogénea que en raíces. Fue importante la detección de Hg en clorénquima donde sus efectos son más tóxicos. Se analizó una correlación entre la distribución de mercurio y calcio y la relación con respuestas fisiológicas. Conclusiones: La fitorremediación de Hg2+ con S. intermedia es una alternativa conveniente. Por haberse realizado en agua real, el protocolo es escalableFil: De La Fournière, Emmanuel M.. Comisión Nacional de Energía Atómica. Centro Atómico Constituyentes. Gerencia de Investigación y Aplicaciones; Argentina. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; ArgentinaFil: Vega, Nahuel Agustín. Comisión Nacional de Energía Atómica. Centro Atómico Constituyentes. Gerencia de Investigación y Aplicaciones; Argentina. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Müller, Nahuel Agustín. Comisión Nacional de Energía Atómica. Centro Atómico Constituyentes. Gerencia de Investigación y Aplicaciones; Argentina. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; ArgentinaFil: Pizarro, Ramón Augusto. Comisión Nacional de Energía Atómica. Gerencia de Área de Aplicaciones de la Tecnología Nuclear. Departamento de Radiobiología; ArgentinaFil: Debray, Mario Ernesto. Comisión Nacional de Energía Atómica. Centro Atómico Constituyentes. Gerencia de Investigación y Aplicaciones; Argentina. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; ArgentinaSociedad Argentina de Botánica2019-06-20info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/122932De La Fournière, Emmanuel M.; Vega, Nahuel Agustín; Müller, Nahuel Agustín; Pizarro, Ramón Augusto; Debray, Mario Ernesto; Determination of mercury in vegetal tissues by microPIXE: Application to the study of hyperaccumulation by Spirodela intermedia (Lemnaceae); Sociedad Argentina de Botánica; Boletín de la Sociedad Argentina de Botánica; 54; 2; 20-6-2019; 263-2750373-580X1851-2372CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://revistas.unc.edu.ar/index.php/BSAB/article/view/24373info:eu-repo/semantics/altIdentifier/doi/10.31055/1851.2372.v54.n2.24373info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T14:17:27Zoai:ri.conicet.gov.ar:11336/122932instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 14:17:27.552CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
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