Reproductive performance of male mice after hypothalamic ghrelin administration

It has been demonstrated that food intake and reproductive physiology are both simultaneously modulated to optimize reproductive success under fluctuating metabolic conditions. Ghrelin (GHRL) is an orexigenic peptide identified as the endogenous ligand of the growth hormone secretagogue receptor tha...

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Autores: Poretti, María Belén, Frautschi, Camila Andrea, Luque, Eugenia Mercedes, Bianconi, Santiago, Martini, Ana Carolina, Stutz, Graciela, Vincenti, Laura María, Santillan, Maria Emilia, Ponzio, Marina Flavia, Schiöth, Helgi, Fiol, Marta Haydee, Carlini, Valeria Paola
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2018
País:Argentina
Institución:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/91548
Acceso en línea:http://hdl.handle.net/11336/91548
Access Level:acceso abierto
Palabra clave:ghrelin
reproduction
testosterone
LH, FSH
https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
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oai_identifier_str oai:ri.conicet.gov.ar:11336/91548
network_acronym_str AR
network_name_str Argentina
repository_id_str
dc.title.none.fl_str_mv Reproductive performance of male mice after hypothalamic ghrelin administration
title Reproductive performance of male mice after hypothalamic ghrelin administration
spellingShingle Reproductive performance of male mice after hypothalamic ghrelin administration
Poretti, María Belén
ghrelin
reproduction
testosterone
LH, FSH
https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
title_short Reproductive performance of male mice after hypothalamic ghrelin administration
title_full Reproductive performance of male mice after hypothalamic ghrelin administration
title_fullStr Reproductive performance of male mice after hypothalamic ghrelin administration
title_full_unstemmed Reproductive performance of male mice after hypothalamic ghrelin administration
title_sort Reproductive performance of male mice after hypothalamic ghrelin administration
dc.creator.none.fl_str_mv Poretti, María Belén
Frautschi, Camila Andrea
Luque, Eugenia Mercedes
Bianconi, Santiago
Martini, Ana Carolina
Stutz, Graciela
Vincenti, Laura María
Santillan, Maria Emilia
Ponzio, Marina Flavia
Schiöth, Helgi
Fiol, Marta Haydee
Carlini, Valeria Paola
author Poretti, María Belén
author_facet Poretti, María Belén
Frautschi, Camila Andrea
Luque, Eugenia Mercedes
Bianconi, Santiago
Martini, Ana Carolina
Stutz, Graciela
Vincenti, Laura María
Santillan, Maria Emilia
Ponzio, Marina Flavia
Schiöth, Helgi
Fiol, Marta Haydee
Carlini, Valeria Paola
author_role author
author2 Frautschi, Camila Andrea
Luque, Eugenia Mercedes
Bianconi, Santiago
Martini, Ana Carolina
Stutz, Graciela
Vincenti, Laura María
Santillan, Maria Emilia
Ponzio, Marina Flavia
Schiöth, Helgi
Fiol, Marta Haydee
Carlini, Valeria Paola
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ghrelin
reproduction
testosterone
LH, FSH
https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
topic ghrelin
reproduction
testosterone
LH, FSH
https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
description It has been demonstrated that food intake and reproductive physiology are both simultaneously modulated to optimize reproductive success under fluctuating metabolic conditions. Ghrelin (GHRL) is an orexigenic peptide identified as the endogenous ligand of the growth hormone secretagogue receptor that is being investigated for its potential role on reproduction. Considering that data available so far are still limited and characterization of GHRL action mechanism on the reproductive system has not been fully elucidated, we studied the participation of hypothalamus in GHRL effects on sperm functional activity, plasma levels of gonadotropins and histological morphology in mice testes after hypothalamic infusion of 0.3 or 3.0 nmol/day GHRL or artificial cerebrospinal fluid (ACSF) at different treatment periods. We found that GHRL 3.0 nmol/day administration for 42 days significantly reduced sperm concentration (GHRL 3.0 nmol/day = 14.05 ± 2.44 × 106/mL vs ACSF = 20.33 ± 1.35 × 106/mL, P< 0.05) and motility (GHRL 3.0 nmol/day = 59.40 ± 4.20% vs ACSF = 75.80 ± 1.40%, P< 0.05). In addition, histological studies showed a significant decrease percentage of spermatogonia (GHRL 3.0 nmol/day = 6.76 ± 0.68% vs ACSF = 9.56 ± 0.41%, P< 0.05) and sperm (GHRL 3.0 nmol/day = 24.24 ± 1.92% vs ACSF = 31.20 ± 3.06%, P< 0.05). These results were associated with a significant reduction in luteinizing hormone and testosterone plasma levels (P< 0.05). As GHRL is an orexigenic peptide, body weight and food intake were measured. Results showed that GHRL increases both parameters; however, the effect did not last beyond the first week of treatment. Results presented in this work confirm that central GHRL administration impairs spermatogenesis and suggest that this effect is mediated by inhibition of hypothalamic–pituitary–gonadal axis.
publishDate 2018
dc.date.none.fl_str_mv 2018-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/91548
Poretti, María Belén; Frautschi, Camila Andrea; Luque, Eugenia Mercedes; Bianconi, Santiago; Martini, Ana Carolina; et al.; Reproductive performance of male mice after hypothalamic ghrelin administration; BioScientifica; Reproduction; 156; 2; 5-2018; 121-132
1470-1626
CONICET Digital
CONICET
url http://hdl.handle.net/11336/91548
identifier_str_mv Poretti, María Belén; Frautschi, Camila Andrea; Luque, Eugenia Mercedes; Bianconi, Santiago; Martini, Ana Carolina; et al.; Reproductive performance of male mice after hypothalamic ghrelin administration; BioScientifica; Reproduction; 156; 2; 5-2018; 121-132
1470-1626
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1530/REP-17-0535
info:eu-repo/semantics/altIdentifier/url/https://rep.bioscientifica.com/view/journals/rep/156/2/REP-17-0535.xml
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv BioScientifica
publisher.none.fl_str_mv BioScientifica
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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spelling Reproductive performance of male mice after hypothalamic ghrelin administrationPoretti, María BelénFrautschi, Camila AndreaLuque, Eugenia MercedesBianconi, SantiagoMartini, Ana CarolinaStutz, GracielaVincenti, Laura MaríaSantillan, Maria EmiliaPonzio, Marina FlaviaSchiöth, HelgiFiol, Marta HaydeeCarlini, Valeria PaolaghrelinreproductiontestosteroneLH, FSHhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3It has been demonstrated that food intake and reproductive physiology are both simultaneously modulated to optimize reproductive success under fluctuating metabolic conditions. Ghrelin (GHRL) is an orexigenic peptide identified as the endogenous ligand of the growth hormone secretagogue receptor that is being investigated for its potential role on reproduction. Considering that data available so far are still limited and characterization of GHRL action mechanism on the reproductive system has not been fully elucidated, we studied the participation of hypothalamus in GHRL effects on sperm functional activity, plasma levels of gonadotropins and histological morphology in mice testes after hypothalamic infusion of 0.3 or 3.0 nmol/day GHRL or artificial cerebrospinal fluid (ACSF) at different treatment periods. We found that GHRL 3.0 nmol/day administration for 42 days significantly reduced sperm concentration (GHRL 3.0 nmol/day = 14.05 ± 2.44 × 106/mL vs ACSF = 20.33 ± 1.35 × 106/mL, P< 0.05) and motility (GHRL 3.0 nmol/day = 59.40 ± 4.20% vs ACSF = 75.80 ± 1.40%, P< 0.05). In addition, histological studies showed a significant decrease percentage of spermatogonia (GHRL 3.0 nmol/day = 6.76 ± 0.68% vs ACSF = 9.56 ± 0.41%, P< 0.05) and sperm (GHRL 3.0 nmol/day = 24.24 ± 1.92% vs ACSF = 31.20 ± 3.06%, P< 0.05). These results were associated with a significant reduction in luteinizing hormone and testosterone plasma levels (P< 0.05). As GHRL is an orexigenic peptide, body weight and food intake were measured. Results showed that GHRL increases both parameters; however, the effect did not last beyond the first week of treatment. Results presented in this work confirm that central GHRL administration impairs spermatogenesis and suggest that this effect is mediated by inhibition of hypothalamic–pituitary–gonadal axis.Fil: Poretti, María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Uppsala University; SueciaFil: Frautschi, Camila Andrea. Universidad Nacional de Córdoba. Facultad de Medicina. Departamento de Fisiología Humana y Física Biomédica. Cátedra de Fisiología Humana; ArgentinaFil: Luque, Eugenia Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Universidad Nacional de Córdoba. Facultad de Medicina. Departamento de Fisiología Humana y Física Biomédica. Cátedra de Fisiología Humana; ArgentinaFil: Bianconi, Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Universidad Nacional de Córdoba. Facultad de Medicina. Departamento de Fisiología Humana y Física Biomédica. Cátedra de Fisiología Humana; ArgentinaFil: Martini, Ana Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; ArgentinaFil: Stutz, Graciela. Universidad Nacional de Córdoba. Facultad de Medicina. Departamento de Fisiología Humana y Física Biomédica. Cátedra de Fisiología Humana; ArgentinaFil: Vincenti, Laura María. Universidad Nacional de Córdoba. Facultad de Medicina. Departamento de Fisiología Humana y Física Biomédica. Cátedra de Fisiología Humana; ArgentinaFil: Santillan, Maria Emilia. Universidad Nacional de Córdoba. Facultad de Medicina. Departamento de Fisiología Humana y Física Biomédica. Cátedra de Fisiología Humana; ArgentinaFil: Ponzio, Marina Flavia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; ArgentinaFil: Schiöth, Helgi. Uppsala University; SueciaFil: Fiol, Marta Haydee. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Universidad Nacional de Córdoba. Facultad de Medicina. Departamento de Fisiología Humana y Física Biomédica. Cátedra de Fisiología Humana; ArgentinaFil: Carlini, Valeria Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Uppsala University; SueciaBioScientifica2018-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/91548Poretti, María Belén; Frautschi, Camila Andrea; Luque, Eugenia Mercedes; Bianconi, Santiago; Martini, Ana Carolina; et al.; Reproductive performance of male mice after hypothalamic ghrelin administration; BioScientifica; Reproduction; 156; 2; 5-2018; 121-1321470-1626CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1530/REP-17-0535info:eu-repo/semantics/altIdentifier/url/https://rep.bioscientifica.com/view/journals/rep/156/2/REP-17-0535.xmlinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T14:16:35Zoai:ri.conicet.gov.ar:11336/91548instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 14:16:35.969CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
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