Proteasas presentes en el látex de frutos de Maclura pomifera (Raf.) Schneid. (Moraceae)
Latex obtained by superficial incisions of Machra pomifera fruits, received on cold neutral phosphate buffer and centrifuged to remove gummy material, affords a crude extract with higher proteolylic activity between pH 8.75 and 10.6 and a notably thermal stability that can be advantageously kept as...
| Autores: | , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión enviada para evaluación y publicación |
| Fecha de publicación: | 1989 |
| País: | Argentina |
| Institución: | Comisión de Investigaciones Científicas de la Provincia de Buenos Aires |
| Repositorio: | CIC Digital (CICBA) |
| Idioma: | español |
| OAI Identifier: | oai:digital.cic.gba.gob.ar:11746/3889 |
| Acceso en línea: | https://digital.cic.gba.gob.ar/handle/11746/3889 |
| Access Level: | acceso abierto |
| Palabra clave: | Farmacología y Farmacia Enzimas fruticultura maclura pomifera (Raf.) Schneid. moraceae fitoproteasas proteasas de látex proteasas de frutos purificación de enzimas plant proteases latex proteases fruit proteases enzyme purification |
| Sumario: | Latex obtained by superficial incisions of Machra pomifera fruits, received on cold neutral phosphate buffer and centrifuged to remove gummy material, affords a crude extract with higher proteolylic activity between pH 8.75 and 10.6 and a notably thermal stability that can be advantageously kept as lyophyhed powder. Acetone precipitation followed by ion exchange chromatography (DEAE-Sepharose CL-6B and CM-Sepharose CL-6B) allows the purification of three active fractions. |
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