A dual death/survival role of autophagy in the adult ovary of Lagostomus maximus (Mammalia- Rodentia)

Follicular atresia is a cell death event that occurs in the great majority of follicles before ovulation in the mature mammalian ovary. Germ cell loss has been mainly associated to apoptosis although autophagy also seems to be at play. Aimed to increase our understanding on the possible cooperating...

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Detalles Bibliográficos
Autores: Leopardo, Noelia Paola, Velazquez, Mariela Edith, Cortasa, Santiago Andrés, Gonzalez, Candela Rocio, Vitullo, Alfredo Daniel
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2020
País:Argentina
Institución:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/139971
Acceso en línea:http://hdl.handle.net/11336/139971
Access Level:acceso abierto
Palabra clave:FOLLICLES
OVARY
AUTOPHAGY
OVULATION
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
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oai_identifier_str oai:ri.conicet.gov.ar:11336/139971
network_acronym_str AR
network_name_str Argentina
repository_id_str
dc.title.none.fl_str_mv A dual death/survival role of autophagy in the adult ovary of Lagostomus maximus (Mammalia- Rodentia)
title A dual death/survival role of autophagy in the adult ovary of Lagostomus maximus (Mammalia- Rodentia)
spellingShingle A dual death/survival role of autophagy in the adult ovary of Lagostomus maximus (Mammalia- Rodentia)
Leopardo, Noelia Paola
FOLLICLES
OVARY
AUTOPHAGY
OVULATION
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
title_short A dual death/survival role of autophagy in the adult ovary of Lagostomus maximus (Mammalia- Rodentia)
title_full A dual death/survival role of autophagy in the adult ovary of Lagostomus maximus (Mammalia- Rodentia)
title_fullStr A dual death/survival role of autophagy in the adult ovary of Lagostomus maximus (Mammalia- Rodentia)
title_full_unstemmed A dual death/survival role of autophagy in the adult ovary of Lagostomus maximus (Mammalia- Rodentia)
title_sort A dual death/survival role of autophagy in the adult ovary of Lagostomus maximus (Mammalia- Rodentia)
dc.creator.none.fl_str_mv Leopardo, Noelia Paola
Velazquez, Mariela Edith
Cortasa, Santiago Andrés
Gonzalez, Candela Rocio
Vitullo, Alfredo Daniel
author Leopardo, Noelia Paola
author_facet Leopardo, Noelia Paola
Velazquez, Mariela Edith
Cortasa, Santiago Andrés
Gonzalez, Candela Rocio
Vitullo, Alfredo Daniel
author_role author
author2 Velazquez, Mariela Edith
Cortasa, Santiago Andrés
Gonzalez, Candela Rocio
Vitullo, Alfredo Daniel
author2_role author
author
author
author
dc.subject.none.fl_str_mv FOLLICLES
OVARY
AUTOPHAGY
OVULATION
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
topic FOLLICLES
OVARY
AUTOPHAGY
OVULATION
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
description Follicular atresia is a cell death event that occurs in the great majority of follicles before ovulation in the mature mammalian ovary. Germ cell loss has been mainly associated to apoptosis although autophagy also seems to be at play. Aimed to increase our understanding on the possible cooperating role of autophagy and apoptosis in follicular atresia and/or follicular survival, we analyzed both programmed cell death mechanisms in a rodent model, the South American plains vizcacha, Lagostomus maximus. Female vizcacha shows highly suppressed apoptosis-dependent follicular atresia in the adult ovary, with continuous folliculogenesis and massive polyovulation. This strategy of massive ovulation requires a permanent remodeling of the ovarian architecture to maintain the availability of quiescent primordial follicles throughout the individual's reproductive lifespan. We report here our analysis of autophagy (BECN1, LAMP1 and LC3B-I/II) and apoptosis (BCL2 and ACTIVE CASPASE- 3) markers which revealed interactive behaviors between both processes, with autophagy promoting survival or cell death depending on the ovarian structure. Strong BECN1, LC3B-II and LAMP1 staining was observed in atretic follicles and degenerating corpora lutea that also expressed nuclear ACTIVE CASPASE-3. Healthy follicles showed a slight expression of autophagy proteins but a strong expression of BCL2 and no detectable ACTIVE CASPASE-3. Transmission electron microscopy revealed a high formation of autophagosomes, autolysosomes and lysosomes in atretic follicles and degenerating corpora lutea and a low number of autophagic vesicles in normal follicles. The co-expression of LC3B-BECN1, LC3B-LAMP1 and LC3B-ACTIVE CASPASE-3 was only detected in atretic follicles and degenerating corpora lutea, while co-expression of BCL2-BECN1 was only observed in normal follicles. We propose that autophagy could act as a mechanism to eliminate altered follicles and remnant corpora lutea providing the necessary space for maturation of primordial follicles that continuously enter the growing follicular pool to sustain massive ovulation.
publishDate 2020
dc.date.none.fl_str_mv 2020-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/139971
Leopardo, Noelia Paola; Velazquez, Mariela Edith; Cortasa, Santiago Andrés; Gonzalez, Candela Rocio; Vitullo, Alfredo Daniel; A dual death/survival role of autophagy in the adult ovary of Lagostomus maximus (Mammalia- Rodentia); Public Library of Science; Plos One; 15; 5; 5-2020; 1-16
1932-6203
CONICET Digital
CONICET
url http://hdl.handle.net/11336/139971
identifier_str_mv Leopardo, Noelia Paola; Velazquez, Mariela Edith; Cortasa, Santiago Andrés; Gonzalez, Candela Rocio; Vitullo, Alfredo Daniel; A dual death/survival role of autophagy in the adult ovary of Lagostomus maximus (Mammalia- Rodentia); Public Library of Science; Plos One; 15; 5; 5-2020; 1-16
1932-6203
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://dx.plos.org/10.1371/journal.pone.0232819
info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0232819
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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spelling A dual death/survival role of autophagy in the adult ovary of Lagostomus maximus (Mammalia- Rodentia)Leopardo, Noelia PaolaVelazquez, Mariela EdithCortasa, Santiago AndrésGonzalez, Candela RocioVitullo, Alfredo DanielFOLLICLESOVARYAUTOPHAGYOVULATIONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Follicular atresia is a cell death event that occurs in the great majority of follicles before ovulation in the mature mammalian ovary. Germ cell loss has been mainly associated to apoptosis although autophagy also seems to be at play. Aimed to increase our understanding on the possible cooperating role of autophagy and apoptosis in follicular atresia and/or follicular survival, we analyzed both programmed cell death mechanisms in a rodent model, the South American plains vizcacha, Lagostomus maximus. Female vizcacha shows highly suppressed apoptosis-dependent follicular atresia in the adult ovary, with continuous folliculogenesis and massive polyovulation. This strategy of massive ovulation requires a permanent remodeling of the ovarian architecture to maintain the availability of quiescent primordial follicles throughout the individual's reproductive lifespan. We report here our analysis of autophagy (BECN1, LAMP1 and LC3B-I/II) and apoptosis (BCL2 and ACTIVE CASPASE- 3) markers which revealed interactive behaviors between both processes, with autophagy promoting survival or cell death depending on the ovarian structure. Strong BECN1, LC3B-II and LAMP1 staining was observed in atretic follicles and degenerating corpora lutea that also expressed nuclear ACTIVE CASPASE-3. Healthy follicles showed a slight expression of autophagy proteins but a strong expression of BCL2 and no detectable ACTIVE CASPASE-3. Transmission electron microscopy revealed a high formation of autophagosomes, autolysosomes and lysosomes in atretic follicles and degenerating corpora lutea and a low number of autophagic vesicles in normal follicles. The co-expression of LC3B-BECN1, LC3B-LAMP1 and LC3B-ACTIVE CASPASE-3 was only detected in atretic follicles and degenerating corpora lutea, while co-expression of BCL2-BECN1 was only observed in normal follicles. We propose that autophagy could act as a mechanism to eliminate altered follicles and remnant corpora lutea providing the necessary space for maturation of primordial follicles that continuously enter the growing follicular pool to sustain massive ovulation.Fil: Leopardo, Noelia Paola. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Maimónides. Área de Investigaciones Biomédicas y Biotecnológicas. Centro de Estudios Biomédicos, Biotecnológicos, Ambientales y de Diagnóstico; ArgentinaFil: Velazquez, Mariela Edith. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Maimónides. Área de Investigaciones Biomédicas y Biotecnológicas. Centro de Estudios Biomédicos, Biotecnológicos, Ambientales y de Diagnóstico; ArgentinaFil: Cortasa, Santiago Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Maimónides. Área de Investigaciones Biomédicas y Biotecnológicas. Centro de Estudios Biomédicos, Biotecnológicos, Ambientales y de Diagnóstico; ArgentinaFil: Gonzalez, Candela Rocio. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Maimónides. Área de Investigaciones Biomédicas y Biotecnológicas. Centro de Estudios Biomédicos, Biotecnológicos, Ambientales y de Diagnóstico; ArgentinaFil: Vitullo, Alfredo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Maimónides. Área de Investigaciones Biomédicas y Biotecnológicas. Centro de Estudios Biomédicos, Biotecnológicos, Ambientales y de Diagnóstico; ArgentinaPublic Library of Science2020-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/139971Leopardo, Noelia Paola; Velazquez, Mariela Edith; Cortasa, Santiago Andrés; Gonzalez, Candela Rocio; Vitullo, Alfredo Daniel; A dual death/survival role of autophagy in the adult ovary of Lagostomus maximus (Mammalia- Rodentia); Public Library of Science; Plos One; 15; 5; 5-2020; 1-161932-6203CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://dx.plos.org/10.1371/journal.pone.0232819info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0232819info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T13:57:13Zoai:ri.conicet.gov.ar:11336/139971instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 13:57:14.071CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
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