Efficient expression of a Toxoplasma gondii dense granule Gra4 antigen in tobacco leaves

A His-tagged truncated version of Toxoplasma gondii dense granule 4 protein (Gra4163-345) was transiently expressed in tobacco leaves. Two genetic constructions were used to accomplish this goal. In one of them, based in a Potato virus X (PVX) amplicon, the sequence encoding His-Gra4163-345 was plac...

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Detalles Bibliográficos
Autores: Ferraro, Gisela, Laguía Becher, Melina, Ángel, Sergio Oscar, Zelada, Alicia Mercedes, Mentaberry, Alejandro Nestor, Clemente, Marina
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2008
País:Argentina
Institución:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/79700
Acceso en línea:http://hdl.handle.net/11336/79700
Access Level:acceso abierto
Palabra clave:GRA4
PLANT TRANSIENT EXPRESSION
PVX AMPLICON
SECRETORY PATHWAY
TOXOPLASMA GONDII
VACUUM INFILTRATION
https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
id AR_9fdb59c499f8f2deb55dfb367bfae2cd
oai_identifier_str oai:ri.conicet.gov.ar:11336/79700
network_acronym_str AR
network_name_str Argentina
repository_id_str
dc.title.none.fl_str_mv Efficient expression of a Toxoplasma gondii dense granule Gra4 antigen in tobacco leaves
title Efficient expression of a Toxoplasma gondii dense granule Gra4 antigen in tobacco leaves
spellingShingle Efficient expression of a Toxoplasma gondii dense granule Gra4 antigen in tobacco leaves
Ferraro, Gisela
GRA4
PLANT TRANSIENT EXPRESSION
PVX AMPLICON
SECRETORY PATHWAY
TOXOPLASMA GONDII
VACUUM INFILTRATION
https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
title_short Efficient expression of a Toxoplasma gondii dense granule Gra4 antigen in tobacco leaves
title_full Efficient expression of a Toxoplasma gondii dense granule Gra4 antigen in tobacco leaves
title_fullStr Efficient expression of a Toxoplasma gondii dense granule Gra4 antigen in tobacco leaves
title_full_unstemmed Efficient expression of a Toxoplasma gondii dense granule Gra4 antigen in tobacco leaves
title_sort Efficient expression of a Toxoplasma gondii dense granule Gra4 antigen in tobacco leaves
dc.creator.none.fl_str_mv Ferraro, Gisela
Laguía Becher, Melina
Ángel, Sergio Oscar
Zelada, Alicia Mercedes
Mentaberry, Alejandro Nestor
Clemente, Marina
author Ferraro, Gisela
author_facet Ferraro, Gisela
Laguía Becher, Melina
Ángel, Sergio Oscar
Zelada, Alicia Mercedes
Mentaberry, Alejandro Nestor
Clemente, Marina
author_role author
author2 Laguía Becher, Melina
Ángel, Sergio Oscar
Zelada, Alicia Mercedes
Mentaberry, Alejandro Nestor
Clemente, Marina
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv GRA4
PLANT TRANSIENT EXPRESSION
PVX AMPLICON
SECRETORY PATHWAY
TOXOPLASMA GONDII
VACUUM INFILTRATION
https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
topic GRA4
PLANT TRANSIENT EXPRESSION
PVX AMPLICON
SECRETORY PATHWAY
TOXOPLASMA GONDII
VACUUM INFILTRATION
https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
description A His-tagged truncated version of Toxoplasma gondii dense granule 4 protein (Gra4163-345) was transiently expressed in tobacco leaves. Two genetic constructions were used to accomplish this goal. In one of them, based in a Potato virus X (PVX) amplicon, the sequence encoding His-Gra4163-345 was placed under control of an additional PVX coat protein subgenomic promoter. In the other, the same sequence was fused to an apoplastic transport signal and placed under the direction of the cauliflower mosaic virus 35S promoter. His-Gra4163-345 accumulation in agroinfiltrated tobacco leaves was estimated by Western blot analysis using mouse anti-Gra4 antibody and a seropositive human serum. Here, we demonstrated the feasibility of producing a Gra4 antigen using transient expression methods in plants.
publishDate 2008
dc.date.none.fl_str_mv 2008-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/79700
Ferraro, Gisela; Laguía Becher, Melina; Ángel, Sergio Oscar; Zelada, Alicia Mercedes; Mentaberry, Alejandro Nestor; et al.; Efficient expression of a Toxoplasma gondii dense granule Gra4 antigen in tobacco leaves; Academic Press Inc Elsevier Science; Experimental Parasitology; 120; 1; 9-2008; 118-122
0014-4894
CONICET Digital
CONICET
url http://hdl.handle.net/11336/79700
identifier_str_mv Ferraro, Gisela; Laguía Becher, Melina; Ángel, Sergio Oscar; Zelada, Alicia Mercedes; Mentaberry, Alejandro Nestor; et al.; Efficient expression of a Toxoplasma gondii dense granule Gra4 antigen in tobacco leaves; Academic Press Inc Elsevier Science; Experimental Parasitology; 120; 1; 9-2008; 118-122
0014-4894
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.exppara.2008.06.002
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0014489408001471
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Academic Press Inc Elsevier Science
publisher.none.fl_str_mv Academic Press Inc Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1799194749887840256
spelling Efficient expression of a Toxoplasma gondii dense granule Gra4 antigen in tobacco leavesFerraro, GiselaLaguía Becher, MelinaÁngel, Sergio OscarZelada, Alicia MercedesMentaberry, Alejandro NestorClemente, MarinaGRA4PLANT TRANSIENT EXPRESSIONPVX AMPLICONSECRETORY PATHWAYTOXOPLASMA GONDIIVACUUM INFILTRATIONhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3A His-tagged truncated version of Toxoplasma gondii dense granule 4 protein (Gra4163-345) was transiently expressed in tobacco leaves. Two genetic constructions were used to accomplish this goal. In one of them, based in a Potato virus X (PVX) amplicon, the sequence encoding His-Gra4163-345 was placed under control of an additional PVX coat protein subgenomic promoter. In the other, the same sequence was fused to an apoplastic transport signal and placed under the direction of the cauliflower mosaic virus 35S promoter. His-Gra4163-345 accumulation in agroinfiltrated tobacco leaves was estimated by Western blot analysis using mouse anti-Gra4 antibody and a seropositive human serum. Here, we demonstrated the feasibility of producing a Gra4 antigen using transient expression methods in plants.Fil: Ferraro, Gisela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); ArgentinaFil: Laguía Becher, Melina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); ArgentinaFil: Ángel, Sergio Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); ArgentinaFil: Zelada, Alicia Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Mentaberry, Alejandro Nestor. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Clemente, Marina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); ArgentinaAcademic Press Inc Elsevier Science2008-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/79700Ferraro, Gisela; Laguía Becher, Melina; Ángel, Sergio Oscar; Zelada, Alicia Mercedes; Mentaberry, Alejandro Nestor; et al.; Efficient expression of a Toxoplasma gondii dense granule Gra4 antigen in tobacco leaves; Academic Press Inc Elsevier Science; Experimental Parasitology; 120; 1; 9-2008; 118-1220014-4894CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.exppara.2008.06.002info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0014489408001471info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T13:35:33Zoai:ri.conicet.gov.ar:11336/79700instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 13:35:34.088CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
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