Determinatiom of 8-Hydroxy 2′-Deoxyguanosine Using Electrodes Modified with a Dispersion of Carbon Nanotubes in Polyethylenimine
Hydroxyl radicals easily oxidize biomolecules such as proteins and DNA. The most abundant oxidative product of DNA is 8-hydroxy 2?-deoxyguanosine (8-OHdG) and this is considered a biomarker of oxidative DNA damage. This work studies the electrochemical behavior of 8-OHdG on electrodes modified with...
| Autores: | , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2011 |
| País: | Argentina |
| Institución: | Consejo Nacional de Investigaciones Científicas y Técnicas |
| Repositorio: | CONICET Digital (CONICET) |
| Idioma: | inglés |
| OAI Identifier: | oai:ri.conicet.gov.ar:11336/66208 |
| Acceso en línea: | http://hdl.handle.net/11336/66208 |
| Access Level: | acceso abierto |
| Palabra clave: | Modified Electrodes Carbon Nanotubes Polyethylenimine 8-Hydroxy 2'-Deoxiguanosine https://purl.org/becyt/ford/1.4 https://purl.org/becyt/ford/1 |
| Sumario: | Hydroxyl radicals easily oxidize biomolecules such as proteins and DNA. The most abundant oxidative product of DNA is 8-hydroxy 2?-deoxyguanosine (8-OHdG) and this is considered a biomarker of oxidative DNA damage. This work studies the electrochemical behavior of 8-OHdG on electrodes modified with carbon nanotubes dispersed in polyethylenimine. The technique of differential pulse anodic stripping voltammetry (DPASV) enables quantification of 8-OHdG in the presence of its major interferents, such as ascorbic acid and uric acid. We obtained linear calibration plots in the range from 5.0x10-7 M to 3.0x10-5 M, with detection limit (DL) of 1.0x10-7 M and the quantification limit (QL) of 3.0x10-7 M. |
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