A novel approach based on capillary electrophoresis coupled to augmented multivariate curve resolution-alternating least-squares modeling for the determination of pKa of 2-hydroxy-4,6-dimethylpyrimidine in nicarbazin
The determination of the dissociation constant of 2-hydroxy-4,6-dimethylpyrimidine in nicarbazin was carried out by means of capillary electrophoresis using a dynamic coating of a fused-silica capillary with high-molecular-mass poly(diallyldimethylammonium chloride) (PDDA) implementing two methods:...
| Autores: | , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2016 |
| País: | Argentina |
| Institución: | Consejo Nacional de Investigaciones Científicas y Técnicas |
| Repositorio: | CONICET Digital (CONICET) |
| Idioma: | inglés |
| OAI Identifier: | oai:ri.conicet.gov.ar:11336/50582 |
| Acceso en línea: | http://hdl.handle.net/11336/50582 |
| Access Level: | acceso abierto |
| Palabra clave: | 2-Hydroxy-4,6-Dimethylpyrimidine Augmented Multivariate Curve Resolution Capillary Electrophoresis Nicarbazin Second-Order Data https://purl.org/becyt/ford/1.4 https://purl.org/becyt/ford/1 |
| Sumario: | The determination of the dissociation constant of 2-hydroxy-4,6-dimethylpyrimidine in nicarbazin was carried out by means of capillary electrophoresis using a dynamic coating of a fused-silica capillary with high-molecular-mass poly(diallyldimethylammonium chloride) (PDDA) implementing two methods: (a) fitting the effective mobility determined from different electrophoretic runs at a single wavelength as a function of pH and (b) a novel modeling strategy of the spectrophotometric second-order data generated with the diode array detector at several wavelengths as a function of pH. The accuracy of the proposed methodologies was verified using robenidine as a model compound with known value of pKa. |
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