Effects of aeration on the synthesis of poly(3-hydroxybutyrate) from glycerol and glucose in recombinant Escherichia coli
Bioreactor cultures of Escherichia coli recombinants carrying phaBAC and phaP of Azotobacter sp. FA8 grown on glycerol under low-agitation conditions accumulated more poly(3-hydroxybutyrate) (PHB) and ethanol than at high agitation, while in glucose cultures, low agitation led to a decrease in PHB f...
| Autores: | , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2010 |
| País: | Argentina |
| Institución: | Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales |
| Repositorio: | Biblioteca Digital (UBA-FCEN) |
| Idioma: | inglés |
| OAI Identifier: | paperaa:paper_00992240_v76_n6_p2036_DeAlmeida |
| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_00992240_v76_n6_p2036_DeAlmeida |
| Access Level: | acceso abierto |
| Palabra clave: | Agitation conditions Batch culture Bioreactor cultures Poly(3-hydroxybutyrate) Recombinant Escherichia coli Relative molecular mass Batch cell culture Cell culture Escherichia coli Ethanol Glycerol Glucose alcohol carboxylic acid glucose glycerol hydroxybutyric acid poly(3 hydroxybutyric acid) poly-beta-hydroxybutyrate polyester recombinant protein aeration bioreactor coliform bacterium ester recombination aerobic metabolism article Azotobacter chemistry fermentation genetics metabolism molecular weight Aerobiosis Bioreactors Carboxylic Acids Fermentation Hydroxybutyrates Molecular Weight Polyesters Recombinant Proteins Bacteria (microorganisms) |
| Sumario: | Bioreactor cultures of Escherichia coli recombinants carrying phaBAC and phaP of Azotobacter sp. FA8 grown on glycerol under low-agitation conditions accumulated more poly(3-hydroxybutyrate) (PHB) and ethanol than at high agitation, while in glucose cultures, low agitation led to a decrease in PHB formation. Cells produced smaller amounts of acids from glycerol than from glucose. Glycerol batch cultures stirred at 125 rpm accumulated, in 24 h, 30.1% (wt/wt) PHB with a relative molecular mass of 1.9 MDa, close to that of PHB obtained using glucose. Copyright © 2010, American Society for Microbiology. All Rights Reserved. |
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