Unveiling the genetic basis of Sclerotinia head rot resistance in sunflower

Background: Sclerotinia sclerotiorum is a necrotrophic fungus that causes Sclerotinia head rot (SHR) in sunflower, with epidemics leading to severe yield losses. In this work, we present an association mapping (AM) approach to investigate the genetic basis of natural resistance to SHR in cultivated...

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Autores: Filippi, Carla Valeria, Zubrzycki, Jeremías Enrique, Di Rienzo, Julio Alejandro, Quiroz, Facundo José, Puebla, Andrea Fabiana, Alvarez, D., Maringolo, C. A., Escande, Alberto Raul, Hopp, Horacio Esteban, Heinz, Ruth Amelia, Paniego, Norma Beatriz, Lia, Veronica Viviana
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2020
País:Argentina
Institución:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/142412
Acceso en línea:http://hdl.handle.net/11336/142412
Access Level:acceso abierto
Palabra clave:ARGENTINIAN GERMPLASM
ASSOCIATION MAPPING
DISEASE RESISTANCE
SUNFLOWER
https://purl.org/becyt/ford/4.4
https://purl.org/becyt/ford/4
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network_name_str Argentina
repository_id_str
dc.title.none.fl_str_mv Unveiling the genetic basis of Sclerotinia head rot resistance in sunflower
title Unveiling the genetic basis of Sclerotinia head rot resistance in sunflower
spellingShingle Unveiling the genetic basis of Sclerotinia head rot resistance in sunflower
Filippi, Carla Valeria
ARGENTINIAN GERMPLASM
ASSOCIATION MAPPING
DISEASE RESISTANCE
SUNFLOWER
https://purl.org/becyt/ford/4.4
https://purl.org/becyt/ford/4
title_short Unveiling the genetic basis of Sclerotinia head rot resistance in sunflower
title_full Unveiling the genetic basis of Sclerotinia head rot resistance in sunflower
title_fullStr Unveiling the genetic basis of Sclerotinia head rot resistance in sunflower
title_full_unstemmed Unveiling the genetic basis of Sclerotinia head rot resistance in sunflower
title_sort Unveiling the genetic basis of Sclerotinia head rot resistance in sunflower
dc.creator.none.fl_str_mv Filippi, Carla Valeria
Zubrzycki, Jeremías Enrique
Di Rienzo, Julio Alejandro
Quiroz, Facundo José
Puebla, Andrea Fabiana
Alvarez, D.
Maringolo, C. A.
Escande, Alberto Raul
Hopp, Horacio Esteban
Heinz, Ruth Amelia
Paniego, Norma Beatriz
Lia, Veronica Viviana
author Filippi, Carla Valeria
author_facet Filippi, Carla Valeria
Zubrzycki, Jeremías Enrique
Di Rienzo, Julio Alejandro
Quiroz, Facundo José
Puebla, Andrea Fabiana
Alvarez, D.
Maringolo, C. A.
Escande, Alberto Raul
Hopp, Horacio Esteban
Heinz, Ruth Amelia
Paniego, Norma Beatriz
Lia, Veronica Viviana
author_role author
author2 Zubrzycki, Jeremías Enrique
Di Rienzo, Julio Alejandro
Quiroz, Facundo José
Puebla, Andrea Fabiana
Alvarez, D.
Maringolo, C. A.
Escande, Alberto Raul
Hopp, Horacio Esteban
Heinz, Ruth Amelia
Paniego, Norma Beatriz
Lia, Veronica Viviana
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ARGENTINIAN GERMPLASM
ASSOCIATION MAPPING
DISEASE RESISTANCE
SUNFLOWER
https://purl.org/becyt/ford/4.4
https://purl.org/becyt/ford/4
topic ARGENTINIAN GERMPLASM
ASSOCIATION MAPPING
DISEASE RESISTANCE
SUNFLOWER
https://purl.org/becyt/ford/4.4
https://purl.org/becyt/ford/4
description Background: Sclerotinia sclerotiorum is a necrotrophic fungus that causes Sclerotinia head rot (SHR) in sunflower, with epidemics leading to severe yield losses. In this work, we present an association mapping (AM) approach to investigate the genetic basis of natural resistance to SHR in cultivated sunflower, the fourth most widely grown oilseed crop in the world. Results: Our association mapping population (AMP), which comprises 135 inbred breeding lines (ILs), was genotyped using 27 candidate genes, a panel of 9 Simple Sequence Repeat (SSR) markers previously associated with SHR resistance via bi-parental mapping, and a set of 384 SNPs located in genes with molecular functions related to stress responses. Moreover, given the complexity of the trait, we evaluated four disease descriptors (i.e, disease incidence, disease severity, area under the disease progress curve for disease incidence, and incubation period). As a result, this work constitutes the most exhaustive AM study of disease resistance in sunflower performed to date. Mixed linear models accounting for population structure and kinship relatedness were used for the statistical analysis of phenotype-genotype associations, allowing the identification of 13 markers associated with disease reduction. The number of favourable alleles was negatively correlated to disease incidence, disease severity and area under the disease progress curve for disease incidence, whereas it was positevily correlated to the incubation period. Conclusions: Four of the markers identified here as associated with SHR resistance (HA1848, HaCOI_1, G33 and G34) validate previous research, while other four novel markers (SNP117, SNP136, SNP44, SNP128) were consistently associated with SHR resistance, emerging as promising candidates for marker-assisted breeding. From the germplasm point of view, the five ILs carrying the largest combination of resistance alleles provide a valuable resource for sunflower breeding programs worldwide.
publishDate 2020
dc.date.none.fl_str_mv 2020-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/142412
Filippi, Carla Valeria; Zubrzycki, Jeremías Enrique; Di Rienzo, Julio Alejandro; Quiroz, Facundo José; Puebla, Andrea Fabiana; et al.; Unveiling the genetic basis of Sclerotinia head rot resistance in sunflower; BioMed Central; BMC Plant Biology; 20; 1; 7-2020; 1-13
1471-2229
CONICET Digital
CONICET
url http://hdl.handle.net/11336/142412
identifier_str_mv Filippi, Carla Valeria; Zubrzycki, Jeremías Enrique; Di Rienzo, Julio Alejandro; Quiroz, Facundo José; Puebla, Andrea Fabiana; et al.; Unveiling the genetic basis of Sclerotinia head rot resistance in sunflower; BioMed Central; BMC Plant Biology; 20; 1; 7-2020; 1-13
1471-2229
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://bmcplantbiol.biomedcentral.com/articles/10.1186/s12870-020-02529-7
info:eu-repo/semantics/altIdentifier/doi/10.1186/s12870-020-02529-7
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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spelling Unveiling the genetic basis of Sclerotinia head rot resistance in sunflowerFilippi, Carla ValeriaZubrzycki, Jeremías EnriqueDi Rienzo, Julio AlejandroQuiroz, Facundo JoséPuebla, Andrea FabianaAlvarez, D.Maringolo, C. A.Escande, Alberto RaulHopp, Horacio EstebanHeinz, Ruth AmeliaPaniego, Norma BeatrizLia, Veronica VivianaARGENTINIAN GERMPLASMASSOCIATION MAPPINGDISEASE RESISTANCESUNFLOWERhttps://purl.org/becyt/ford/4.4https://purl.org/becyt/ford/4Background: Sclerotinia sclerotiorum is a necrotrophic fungus that causes Sclerotinia head rot (SHR) in sunflower, with epidemics leading to severe yield losses. In this work, we present an association mapping (AM) approach to investigate the genetic basis of natural resistance to SHR in cultivated sunflower, the fourth most widely grown oilseed crop in the world. Results: Our association mapping population (AMP), which comprises 135 inbred breeding lines (ILs), was genotyped using 27 candidate genes, a panel of 9 Simple Sequence Repeat (SSR) markers previously associated with SHR resistance via bi-parental mapping, and a set of 384 SNPs located in genes with molecular functions related to stress responses. Moreover, given the complexity of the trait, we evaluated four disease descriptors (i.e, disease incidence, disease severity, area under the disease progress curve for disease incidence, and incubation period). As a result, this work constitutes the most exhaustive AM study of disease resistance in sunflower performed to date. Mixed linear models accounting for population structure and kinship relatedness were used for the statistical analysis of phenotype-genotype associations, allowing the identification of 13 markers associated with disease reduction. The number of favourable alleles was negatively correlated to disease incidence, disease severity and area under the disease progress curve for disease incidence, whereas it was positevily correlated to the incubation period. Conclusions: Four of the markers identified here as associated with SHR resistance (HA1848, HaCOI_1, G33 and G34) validate previous research, while other four novel markers (SNP117, SNP136, SNP44, SNP128) were consistently associated with SHR resistance, emerging as promising candidates for marker-assisted breeding. From the germplasm point of view, the five ILs carrying the largest combination of resistance alleles provide a valuable resource for sunflower breeding programs worldwide.Fil: Filippi, Carla Valeria. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Zubrzycki, Jeremías Enrique. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Di Rienzo, Julio Alejandro. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias; ArgentinaFil: Quiroz, Facundo José. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce; ArgentinaFil: Puebla, Andrea Fabiana. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Alvarez, D.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Córdoba. Estación Experimental Agropecuaria Manfredi; ArgentinaFil: Maringolo, C. A.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce; ArgentinaFil: Escande, Alberto Raul. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce; ArgentinaFil: Hopp, Horacio Esteban. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Heinz, Ruth Amelia. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Paniego, Norma Beatriz. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Lia, Veronica Viviana. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; ArgentinaBioMed Central2020-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/142412Filippi, Carla Valeria; Zubrzycki, Jeremías Enrique; Di Rienzo, Julio Alejandro; Quiroz, Facundo José; Puebla, Andrea Fabiana; et al.; Unveiling the genetic basis of Sclerotinia head rot resistance in sunflower; BioMed Central; BMC Plant Biology; 20; 1; 7-2020; 1-131471-2229CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://bmcplantbiol.biomedcentral.com/articles/10.1186/s12870-020-02529-7info:eu-repo/semantics/altIdentifier/doi/10.1186/s12870-020-02529-7info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T13:43:30Zoai:ri.conicet.gov.ar:11336/142412instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 13:43:31.063CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
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