Rotavirus VP6 protein expressed in cell culture by HSV-1-based vectors
HSV-1 vectors were developed for the expression of several rotavirus genes. Genes from other pathogens were also expressed, thus generating a set of tools to be tested as new vaccine candidates against In this section, we show two different HSV-1 vectors that express the VP6 protein from different r...
| Autores: | , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2015 |
| País: | Argentina |
| Institución: | Consejo Nacional de Investigaciones Científicas y Técnicas |
| Repositorio: | CONICET Digital (CONICET) |
| Idioma: | inglés |
| OAI Identifier: | oai:ri.conicet.gov.ar:11336/4043 |
| Acceso en línea: | http://hdl.handle.net/11336/4043 |
| Access Level: | acceso abierto |
| Palabra clave: | herpesvirus vectors rotavirus VP6 recombinant protein https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
| Sumario: | HSV-1 vectors were developed for the expression of several rotavirus genes. Genes from other pathogens were also expressed, thus generating a set of tools to be tested as new vaccine candidates against In this section, we show two different HSV-1 vectors that express the VP6 protein from different rotavirus strains. Figure 1 shows a defective recombinant virus expressing simultaneously EGFP and VP6 from murine rotavirus strain EC, In Figure 2, an amplicon vector overexpressing VP6 from a Wa strain of human Rotavirus is shown. In both cases, the VP6 proteins were recognized by a monoclonal antibody and were found extensively expressed forming filaments along the cells, showing a typical intra-cellular pattern, in the absence of other rotavirus proteins. These results show that the recombinant proteins maintain the specific capacity of self-assembly of the native protein and, therefore, are potential vaccine candidates to be explored. |
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