Influence of vitamins and osmolites on growth and bacteriocin production by Lactobacillus salivarius CRL 1328 in a chemically defined medium
The aim of this study was to analyze the influence of vitamins, glycerol and salts on the growth and bacteriocin production by Lactobacillus salivarius CRL 1328, a human vagina isolate, by using a chemically defined medium, in order to define the optimal conditions for salivaricin production. The si...
| Authors: | , , , |
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| Format: | article |
| Status: | Published version |
| Publication Date: | 2009 |
| Country: | Argentina |
| Institution: | Consejo Nacional de Investigaciones Científicas y Técnicas |
| Repository: | CONICET Digital (CONICET) |
| Language: | English |
| OAI Identifier: | oai:ri.conicet.gov.ar:11336/53194 |
| Online Access: | http://hdl.handle.net/11336/53194 |
| Access Level: | Open access |
| Keyword: | Bacteriocin Lactic Acid Bacteria Lactobacillus Salivaricin Vagina https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
| Summary: | The aim of this study was to analyze the influence of vitamins, glycerol and salts on the growth and bacteriocin production by Lactobacillus salivarius CRL 1328, a human vagina isolate, by using a chemically defined medium, in order to define the optimal conditions for salivaricin production. The single omission of d-biotin, thiamine, p-aminobenzoic acid, folic acid or cyanocobalamin did not affect the bacterial growth, while the removal of nicotinic acid, riboflavin and pyridoxal produced a decrease of about 30% in the growth rate. Maximum salivaricin activity was observed after the addition of 5 or 10 g/l of NaCl. On the basis of the nutritional requirements and the levels of salivaricin production, a new optimized and simplified defined medium (SDM-NaCl) for L. salivarius CRL 1328 bacteriocin production was formulated. The kinetics of salivaricin production in SDM-NaCl and in the complex media LAPTg revealed that bacteriocin production was growth linked. A combination of Tricine-SDS-PAGE, Lumitein protein gel staining and a bioassay for antibacterial activity indicated that the molecular weight of salivaricin CRL 1328 is about 4.5 kDa. The partially purified bacteriocin, obtained from SDM-NaCl after concentration, allowed designing a relatively simple method for the recovery of a biologically active protein. |
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