Alterations of the Coxiella burnetii replicative vacuole membrane integrity and interplay with the autophagy pathway

Coxiella burnetii, the etiologic agent of Q fever, is a Gram-negative obligate intracellular bacterium. It has been previously described that both the endocytic and autophagic pathways contribute to the Coxiella replicative vacuole (CRV) generation. Galectins are β-galactoside-binding lectins that a...

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Authors: Mansilla Pareja, Maria Eugenia, Bongiovanni, Antonino, Lafont, Frank, Colombo, Maria Isabel
Format: article
Status:Published version
Publication Date:2017
Country:Argentina
Institution:Consejo Nacional de Investigaciones Científicas y Técnicas
Repository:CONICET Digital (CONICET)
Language:English
OAI Identifier:oai:ri.conicet.gov.ar:11336/49658
Online Access:http://hdl.handle.net/11336/49658
Access Level:Open access
Keyword:AUTOPHAGY
COXIELLA BURNETII
DAMAGE DETECTION
GALECTINS
VACUOLE MEMBRANE
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
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oai_identifier_str oai:ri.conicet.gov.ar:11336/49658
network_acronym_str AR
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repository_id_str
spelling Alterations of the Coxiella burnetii replicative vacuole membrane integrity and interplay with the autophagy pathwayMansilla Pareja, Maria EugeniaBongiovanni, AntoninoLafont, FrankColombo, Maria IsabelAUTOPHAGYCOXIELLA BURNETIIDAMAGE DETECTIONGALECTINSVACUOLE MEMBRANEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Coxiella burnetii, the etiologic agent of Q fever, is a Gram-negative obligate intracellular bacterium. It has been previously described that both the endocytic and autophagic pathways contribute to the Coxiella replicative vacuole (CRV) generation. Galectins are β-galactoside-binding lectins that accumulate in the cytosol before being secreted via a non-conventional secretory pathway. It has been shown that Galectin-3, -8, -9 monitor bacteria vacuolar rupture and endosomal and lysosomal loss of membrane integrity through binding of host glycans exposed in the cytoplasm after membrane damage. Using microinjection of fluorescence-coupled dextrans, a FRET assay, and galectins distribution, we demonstrate that Coxiella infection actually result in transient phagosomal/CRV membrane damage in a Dot/Icm-dependent manner. We also show the association of different adaptor molecules involved in autophagy and of LC3 to the limiting membrane of the CRV. Moreover, we show that upon autophagy inhibition, the proportion of CRVs labeled with galectins and less acidified increases which is associated with bacteria replication impairment. Based on these observations, we propose that autophagy can facilitate resealing of intracellular damaged membranes.Fil: Mansilla Pareja, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; ArgentinaFil: Bongiovanni, Antonino. Instituto Pasteur; FranciaFil: Lafont, Frank. Instituto Pasteur; FranciaFil: Colombo, Maria Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; ArgentinaFrontiers Research Foundation2017-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/49658Mansilla Pareja, Maria Eugenia; Bongiovanni, Antonino; Lafont, Frank; Colombo, Maria Isabel; Alterations of the Coxiella burnetii replicative vacuole membrane integrity and interplay with the autophagy pathway; Frontiers Research Foundation; Frontiers in Cellular and Infection Microbiology; 7; 112; 4-2017; 1-172235-29882235-2988CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://journal.frontiersin.org/article/10.3389/fcimb.2017.00112/fullinfo:eu-repo/semantics/altIdentifier/doi/10.3389/fcimb.2017.00112info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T14:20:46Zoai:ri.conicet.gov.ar:11336/49658instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 14:20:46.522CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Alterations of the Coxiella burnetii replicative vacuole membrane integrity and interplay with the autophagy pathway
title Alterations of the Coxiella burnetii replicative vacuole membrane integrity and interplay with the autophagy pathway
spellingShingle Alterations of the Coxiella burnetii replicative vacuole membrane integrity and interplay with the autophagy pathway
Mansilla Pareja, Maria Eugenia
AUTOPHAGY
COXIELLA BURNETII
DAMAGE DETECTION
GALECTINS
VACUOLE MEMBRANE
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
title_short Alterations of the Coxiella burnetii replicative vacuole membrane integrity and interplay with the autophagy pathway
title_full Alterations of the Coxiella burnetii replicative vacuole membrane integrity and interplay with the autophagy pathway
title_fullStr Alterations of the Coxiella burnetii replicative vacuole membrane integrity and interplay with the autophagy pathway
title_full_unstemmed Alterations of the Coxiella burnetii replicative vacuole membrane integrity and interplay with the autophagy pathway
title_sort Alterations of the Coxiella burnetii replicative vacuole membrane integrity and interplay with the autophagy pathway
dc.creator.none.fl_str_mv Mansilla Pareja, Maria Eugenia
Bongiovanni, Antonino
Lafont, Frank
Colombo, Maria Isabel
author Mansilla Pareja, Maria Eugenia
author_facet Mansilla Pareja, Maria Eugenia
Bongiovanni, Antonino
Lafont, Frank
Colombo, Maria Isabel
author_role author
author2 Bongiovanni, Antonino
Lafont, Frank
Colombo, Maria Isabel
author2_role author
author
author
dc.subject.none.fl_str_mv AUTOPHAGY
COXIELLA BURNETII
DAMAGE DETECTION
GALECTINS
VACUOLE MEMBRANE
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
topic AUTOPHAGY
COXIELLA BURNETII
DAMAGE DETECTION
GALECTINS
VACUOLE MEMBRANE
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
description Coxiella burnetii, the etiologic agent of Q fever, is a Gram-negative obligate intracellular bacterium. It has been previously described that both the endocytic and autophagic pathways contribute to the Coxiella replicative vacuole (CRV) generation. Galectins are β-galactoside-binding lectins that accumulate in the cytosol before being secreted via a non-conventional secretory pathway. It has been shown that Galectin-3, -8, -9 monitor bacteria vacuolar rupture and endosomal and lysosomal loss of membrane integrity through binding of host glycans exposed in the cytoplasm after membrane damage. Using microinjection of fluorescence-coupled dextrans, a FRET assay, and galectins distribution, we demonstrate that Coxiella infection actually result in transient phagosomal/CRV membrane damage in a Dot/Icm-dependent manner. We also show the association of different adaptor molecules involved in autophagy and of LC3 to the limiting membrane of the CRV. Moreover, we show that upon autophagy inhibition, the proportion of CRVs labeled with galectins and less acidified increases which is associated with bacteria replication impairment. Based on these observations, we propose that autophagy can facilitate resealing of intracellular damaged membranes.
publishDate 2017
dc.date.none.fl_str_mv 2017-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/49658
Mansilla Pareja, Maria Eugenia; Bongiovanni, Antonino; Lafont, Frank; Colombo, Maria Isabel; Alterations of the Coxiella burnetii replicative vacuole membrane integrity and interplay with the autophagy pathway; Frontiers Research Foundation; Frontiers in Cellular and Infection Microbiology; 7; 112; 4-2017; 1-17
2235-2988
2235-2988
CONICET Digital
CONICET
url http://hdl.handle.net/11336/49658
identifier_str_mv Mansilla Pareja, Maria Eugenia; Bongiovanni, Antonino; Lafont, Frank; Colombo, Maria Isabel; Alterations of the Coxiella burnetii replicative vacuole membrane integrity and interplay with the autophagy pathway; Frontiers Research Foundation; Frontiers in Cellular and Infection Microbiology; 7; 112; 4-2017; 1-17
2235-2988
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://journal.frontiersin.org/article/10.3389/fcimb.2017.00112/full
info:eu-repo/semantics/altIdentifier/doi/10.3389/fcimb.2017.00112
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Frontiers Research Foundation
publisher.none.fl_str_mv Frontiers Research Foundation
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1799196259681042432
score 15.812429

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