Postsynaptic recordings at afferent dendrites contacting cochlear inner hair cells: Monitoring multivesicular release at a ribbon synapse

The afferent synapse between the inner hair cell (IHC) and the auditory nerve fiber provides an electrophysiologically accessible site for recording the postsynaptic activity of a single ribbon synapse. Ribbon synapses of sensory cells release neurotransmitter continuously, the rate of which is modu...

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Detalhes bibliográficos
Autores: Grant, Lisa, Yi, Eunyoung, Goutman, Juan Diego, Glowatzki, Elisabeth
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2011
País:Argentina
Recursos:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/79442
Acesso em linha:http://hdl.handle.net/11336/79442
Access Level:acceso abierto
Palavra-chave:RIBBON SYNAPSE
DENDRITE
MULTIVESICULAR RELEASE
HAIR CELL
https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
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network_acronym_str AR
network_name_str Argentina
repository_id_str
spelling Postsynaptic recordings at afferent dendrites contacting cochlear inner hair cells: Monitoring multivesicular release at a ribbon synapseGrant, LisaYi, EunyoungGoutman, Juan DiegoGlowatzki, ElisabethRIBBON SYNAPSEDENDRITEMULTIVESICULAR RELEASEHAIR CELLhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3The afferent synapse between the inner hair cell (IHC) and the auditory nerve fiber provides an electrophysiologically accessible site for recording the postsynaptic activity of a single ribbon synapse. Ribbon synapses of sensory cells release neurotransmitter continuously, the rate of which is modulated in response to graded changes in IHC membrane potential. Ribbon synapses have been shown to operate by multivesicular release, where multiple vesicles can be released simultaneously to evoke excitatory postsynaptic currents (EPSCs) of varying amplitudes. Neither the role of the presynaptic ribbon, nor the mechanism underlying multivesicular release is currently well understood. The IHC is innervated by 10-20 auditory nerve fibers, and every fiber contacts the IHC with a unmyelinated single ending to form a single ribbon synapse. The small size of the afferent boutons contacting IHCs (approximately 1 μm in diameter) enables recordings with exceptional temporal resolution to be made. Furthermore, the technique can be adapted to record from both pre- and postsynaptic cells simultaneously, allowing the transfer function at the synapse to be studied directly. This method therefore provides a means by which fundamental aspects of neurotransmission can be studied, from multivesicular release to the elusive function of the ribbon in sensory cells.Fil: Grant, Lisa. The Johns Hopkins School of Medicine; Estados UnidosFil: Yi, Eunyoung. The Johns Hopkins School of Medicine; Estados UnidosFil: Goutman, Juan Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Glowatzki, Elisabeth. The Johns Hopkins School of Medicine; Estados UnidosJournal of Visualized Experiments2011-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/79442Grant, Lisa; Yi, Eunyoung; Goutman, Juan Diego; Glowatzki, Elisabeth; Postsynaptic recordings at afferent dendrites contacting cochlear inner hair cells: Monitoring multivesicular release at a ribbon synapse; Journal of Visualized Experiments; Journal of Visualized Experiments; 48; 2-2011; 1-71940-087XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.3791/2442info:eu-repo/semantics/altIdentifier/url/https://www.jove.com/video/2442/postsynaptic-recordings-at-afferent-dendrites-contacting-cochlearinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T14:15:15Zoai:ri.conicet.gov.ar:11336/79442instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 14:15:15.361CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Postsynaptic recordings at afferent dendrites contacting cochlear inner hair cells: Monitoring multivesicular release at a ribbon synapse
title Postsynaptic recordings at afferent dendrites contacting cochlear inner hair cells: Monitoring multivesicular release at a ribbon synapse
spellingShingle Postsynaptic recordings at afferent dendrites contacting cochlear inner hair cells: Monitoring multivesicular release at a ribbon synapse
Grant, Lisa
RIBBON SYNAPSE
DENDRITE
MULTIVESICULAR RELEASE
HAIR CELL
https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
title_short Postsynaptic recordings at afferent dendrites contacting cochlear inner hair cells: Monitoring multivesicular release at a ribbon synapse
title_full Postsynaptic recordings at afferent dendrites contacting cochlear inner hair cells: Monitoring multivesicular release at a ribbon synapse
title_fullStr Postsynaptic recordings at afferent dendrites contacting cochlear inner hair cells: Monitoring multivesicular release at a ribbon synapse
title_full_unstemmed Postsynaptic recordings at afferent dendrites contacting cochlear inner hair cells: Monitoring multivesicular release at a ribbon synapse
title_sort Postsynaptic recordings at afferent dendrites contacting cochlear inner hair cells: Monitoring multivesicular release at a ribbon synapse
dc.creator.none.fl_str_mv Grant, Lisa
Yi, Eunyoung
Goutman, Juan Diego
Glowatzki, Elisabeth
author Grant, Lisa
author_facet Grant, Lisa
Yi, Eunyoung
Goutman, Juan Diego
Glowatzki, Elisabeth
author_role author
author2 Yi, Eunyoung
Goutman, Juan Diego
Glowatzki, Elisabeth
author2_role author
author
author
dc.subject.none.fl_str_mv RIBBON SYNAPSE
DENDRITE
MULTIVESICULAR RELEASE
HAIR CELL
https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
topic RIBBON SYNAPSE
DENDRITE
MULTIVESICULAR RELEASE
HAIR CELL
https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
description The afferent synapse between the inner hair cell (IHC) and the auditory nerve fiber provides an electrophysiologically accessible site for recording the postsynaptic activity of a single ribbon synapse. Ribbon synapses of sensory cells release neurotransmitter continuously, the rate of which is modulated in response to graded changes in IHC membrane potential. Ribbon synapses have been shown to operate by multivesicular release, where multiple vesicles can be released simultaneously to evoke excitatory postsynaptic currents (EPSCs) of varying amplitudes. Neither the role of the presynaptic ribbon, nor the mechanism underlying multivesicular release is currently well understood. The IHC is innervated by 10-20 auditory nerve fibers, and every fiber contacts the IHC with a unmyelinated single ending to form a single ribbon synapse. The small size of the afferent boutons contacting IHCs (approximately 1 μm in diameter) enables recordings with exceptional temporal resolution to be made. Furthermore, the technique can be adapted to record from both pre- and postsynaptic cells simultaneously, allowing the transfer function at the synapse to be studied directly. This method therefore provides a means by which fundamental aspects of neurotransmission can be studied, from multivesicular release to the elusive function of the ribbon in sensory cells.
publishDate 2011
dc.date.none.fl_str_mv 2011-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/79442
Grant, Lisa; Yi, Eunyoung; Goutman, Juan Diego; Glowatzki, Elisabeth; Postsynaptic recordings at afferent dendrites contacting cochlear inner hair cells: Monitoring multivesicular release at a ribbon synapse; Journal of Visualized Experiments; Journal of Visualized Experiments; 48; 2-2011; 1-7
1940-087X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/79442
identifier_str_mv Grant, Lisa; Yi, Eunyoung; Goutman, Juan Diego; Glowatzki, Elisabeth; Postsynaptic recordings at afferent dendrites contacting cochlear inner hair cells: Monitoring multivesicular release at a ribbon synapse; Journal of Visualized Experiments; Journal of Visualized Experiments; 48; 2-2011; 1-7
1940-087X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.3791/2442
info:eu-repo/semantics/altIdentifier/url/https://www.jove.com/video/2442/postsynaptic-recordings-at-afferent-dendrites-contacting-cochlear
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Journal of Visualized Experiments
publisher.none.fl_str_mv Journal of Visualized Experiments
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 15,812455

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