Dehydroleucodine and dehydroparishin-B inhibit proliferation and motility of B16 melanoma cells

Dehydroleucodine, a known sesquiterpene lactone, and dehydroparishin-B, a new guaiane type sesquiterpene acid, were isolated from aerial parts of Artemisia douglasiana by chloroform extraction. We identified dehydroparishin-B as (7R)-2-oxo-guaia-1(10),3(4),5(6),11(13)-tetraen-12-oic acid by MS and N...

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Detalles Bibliográficos
Autores: Priestap, Horacio A., Galvis, Adriana, Rivero, Nathalie, Costantino, Valeria Victoria, Lopez, Luis Alberto, Barbieri, M. Alejandro
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2012
País:Argentina
Institución:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/142108
Acceso en línea:http://hdl.handle.net/11336/142108
Access Level:acceso abierto
Palabra clave:ARTEMISIA DOUGLASIANA BESSER
B16 MELANOMA CELLS
MELAN-A
MIGRATION
PROLIFERATION
SESQUITERPENES
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
Descripción
Sumario:Dehydroleucodine, a known sesquiterpene lactone, and dehydroparishin-B, a new guaiane type sesquiterpene acid, were isolated from aerial parts of Artemisia douglasiana by chloroform extraction. We identified dehydroparishin-B as (7R)-2-oxo-guaia-1(10),3(4),5(6),11(13)-tetraen-12-oic acid by MS and NMR methods. We demonstrated that both dehydroparishin-B and dehydroleucodine blocked cell proliferation of B16 melanoma cells, but not normal murine Melan-A melanocytes, in a dose-dependent manner without affecting cell viability. We also found that both dehydroparishin-B and dehydroleucodine inhibited migration of B16 melanoma cells. These results suggest that dehydroleucodine and dehydroparishin-B could represent potential candidates for the treatment of metastatic melanomas.