Re-evaluation of the steady-state self-quenching constant of quinine bisulphate from fluorescence measurements in transmission geometry

In the present work we show that a recent methodology developed by us to acquire emission spectra and fluorescence quantum yields of highly absorbing samples in transmission configuration, constitutes a very simple and robust alternative to determine self-quenching constants, KSQ. We measured the ab...

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Detalles Bibliográficos
Autores: Krimer, Nicolas, Mirenda, Martin
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2017
País:Argentina
Institución:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/76980
Acceso en línea:http://hdl.handle.net/11336/76980
Access Level:acceso abierto
Palabra clave:FLUORESCENCE
QUININE
RE-ABSORPTION
SELF-QUENCHING
https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
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network_acronym_str AR
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repository_id_str
spelling Re-evaluation of the steady-state self-quenching constant of quinine bisulphate from fluorescence measurements in transmission geometryKrimer, NicolasMirenda, MartinFLUORESCENCEQUININERE-ABSORPTIONSELF-QUENCHINGhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1In the present work we show that a recent methodology developed by us to acquire emission spectra and fluorescence quantum yields of highly absorbing samples in transmission configuration, constitutes a very simple and robust alternative to determine self-quenching constants, KSQ. We measured the absorption and the steady-state emission spectra of quinine bisulphate, QBS, solutions ranging between 1.5 × 10-5 and 1.5 × 10-1 M. From these data, we calculated the expected emission spectra, affected by re-absorption, for all QBS concentrations. For higher concentrations, the re-absorption in the excitation/detection direction reaches values up to 6% of the total emitted intensity. The KSQ of the dye was reevaluated from the concentration dependence of the quotients between the calculated and the experimental integrated emission spectra. The obtained value, KSQ = 18.4 ± 0.1 M-1, shows no significant differences with those obtained from steady-state and average lifetimes by other authors, pointing out the diffusional nature of the self-quenching phenomenon. The present work helps clarify some ambiguous aspects concerning the photophysics of QBS, stressing that re-absorption phenomena must be considered in QBS concentrated solutions for accuracy measurements.Fil: Krimer, Nicolas. Comisión Nacional de Energía Atómica. Centro Atómico Constituyentes; ArgentinaFil: Mirenda, Martin. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Inorgánica, Analítica y Química Física; Argentina. Comisión Nacional de Energía Atómica. Centro Atómico Constituyentes; ArgentinaIOP Publishing Ltd2017-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/76980Krimer, Nicolas; Mirenda, Martin; Re-evaluation of the steady-state self-quenching constant of quinine bisulphate from fluorescence measurements in transmission geometry; IOP Publishing Ltd; Methods and Applications in Fluorescence; 5; 3; 9-2017; 1-72050-6120CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://iopscience.iop.org/article/10.1088/2050-6120/aa72a9info:eu-repo/semantics/altIdentifier/doi/10.1088/2050-6120/aa72a9info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T13:36:20Zoai:ri.conicet.gov.ar:11336/76980instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 13:36:21.222CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Re-evaluation of the steady-state self-quenching constant of quinine bisulphate from fluorescence measurements in transmission geometry
title Re-evaluation of the steady-state self-quenching constant of quinine bisulphate from fluorescence measurements in transmission geometry
spellingShingle Re-evaluation of the steady-state self-quenching constant of quinine bisulphate from fluorescence measurements in transmission geometry
Krimer, Nicolas
FLUORESCENCE
QUININE
RE-ABSORPTION
SELF-QUENCHING
https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
title_short Re-evaluation of the steady-state self-quenching constant of quinine bisulphate from fluorescence measurements in transmission geometry
title_full Re-evaluation of the steady-state self-quenching constant of quinine bisulphate from fluorescence measurements in transmission geometry
title_fullStr Re-evaluation of the steady-state self-quenching constant of quinine bisulphate from fluorescence measurements in transmission geometry
title_full_unstemmed Re-evaluation of the steady-state self-quenching constant of quinine bisulphate from fluorescence measurements in transmission geometry
title_sort Re-evaluation of the steady-state self-quenching constant of quinine bisulphate from fluorescence measurements in transmission geometry
dc.creator.none.fl_str_mv Krimer, Nicolas
Mirenda, Martin
author Krimer, Nicolas
author_facet Krimer, Nicolas
Mirenda, Martin
author_role author
author2 Mirenda, Martin
author2_role author
dc.subject.none.fl_str_mv FLUORESCENCE
QUININE
RE-ABSORPTION
SELF-QUENCHING
https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
topic FLUORESCENCE
QUININE
RE-ABSORPTION
SELF-QUENCHING
https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
description In the present work we show that a recent methodology developed by us to acquire emission spectra and fluorescence quantum yields of highly absorbing samples in transmission configuration, constitutes a very simple and robust alternative to determine self-quenching constants, KSQ. We measured the absorption and the steady-state emission spectra of quinine bisulphate, QBS, solutions ranging between 1.5 × 10-5 and 1.5 × 10-1 M. From these data, we calculated the expected emission spectra, affected by re-absorption, for all QBS concentrations. For higher concentrations, the re-absorption in the excitation/detection direction reaches values up to 6% of the total emitted intensity. The KSQ of the dye was reevaluated from the concentration dependence of the quotients between the calculated and the experimental integrated emission spectra. The obtained value, KSQ = 18.4 ± 0.1 M-1, shows no significant differences with those obtained from steady-state and average lifetimes by other authors, pointing out the diffusional nature of the self-quenching phenomenon. The present work helps clarify some ambiguous aspects concerning the photophysics of QBS, stressing that re-absorption phenomena must be considered in QBS concentrated solutions for accuracy measurements.
publishDate 2017
dc.date.none.fl_str_mv 2017-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/76980
Krimer, Nicolas; Mirenda, Martin; Re-evaluation of the steady-state self-quenching constant of quinine bisulphate from fluorescence measurements in transmission geometry; IOP Publishing Ltd; Methods and Applications in Fluorescence; 5; 3; 9-2017; 1-7
2050-6120
CONICET Digital
CONICET
url http://hdl.handle.net/11336/76980
identifier_str_mv Krimer, Nicolas; Mirenda, Martin; Re-evaluation of the steady-state self-quenching constant of quinine bisulphate from fluorescence measurements in transmission geometry; IOP Publishing Ltd; Methods and Applications in Fluorescence; 5; 3; 9-2017; 1-7
2050-6120
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://iopscience.iop.org/article/10.1088/2050-6120/aa72a9
info:eu-repo/semantics/altIdentifier/doi/10.1088/2050-6120/aa72a9
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv IOP Publishing Ltd
publisher.none.fl_str_mv IOP Publishing Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 15,812429

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