PDMS-based porous particles as support beds for cell immobilization: Bacterial biofilm formation as a function of porosity and polymer composition

The objective of this work is to test the performance of new synthetic polydimethylsiloxane (PDMS)-based bed particles acting as carriers for bacteria biofilms. The particles obtained have a highly interconnected porous structure which offers a large surface adsorption area to the bacteria. In addit...

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Detalhes bibliográficos
Autores: Fernandez, Marcelo Raul, Casabona, Maria Guillermina, Anupama, V. N., Krishnakumar, B., Curutchet, Gustavo Andres, Bernik, Delia Leticia
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2010
País:Argentina
Recursos:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/71586
Acesso em linha:http://hdl.handle.net/11336/71586
Access Level:acceso abierto
Palavra-chave:Bed Material
Biofilm
Cslm
Pdms
Sem
https://purl.org/becyt/ford/2.8
https://purl.org/becyt/ford/2
id AR_951b366e9e830ead179b6033cd33b64b
oai_identifier_str oai:ri.conicet.gov.ar:11336/71586
network_acronym_str AR
network_name_str Argentina
repository_id_str
dc.title.none.fl_str_mv PDMS-based porous particles as support beds for cell immobilization: Bacterial biofilm formation as a function of porosity and polymer composition
title PDMS-based porous particles as support beds for cell immobilization: Bacterial biofilm formation as a function of porosity and polymer composition
spellingShingle PDMS-based porous particles as support beds for cell immobilization: Bacterial biofilm formation as a function of porosity and polymer composition
Fernandez, Marcelo Raul
Bed Material
Biofilm
Cslm
Pdms
Sem
https://purl.org/becyt/ford/2.8
https://purl.org/becyt/ford/2
title_short PDMS-based porous particles as support beds for cell immobilization: Bacterial biofilm formation as a function of porosity and polymer composition
title_full PDMS-based porous particles as support beds for cell immobilization: Bacterial biofilm formation as a function of porosity and polymer composition
title_fullStr PDMS-based porous particles as support beds for cell immobilization: Bacterial biofilm formation as a function of porosity and polymer composition
title_full_unstemmed PDMS-based porous particles as support beds for cell immobilization: Bacterial biofilm formation as a function of porosity and polymer composition
title_sort PDMS-based porous particles as support beds for cell immobilization: Bacterial biofilm formation as a function of porosity and polymer composition
dc.creator.none.fl_str_mv Fernandez, Marcelo Raul
Casabona, Maria Guillermina
Anupama, V. N.
Krishnakumar, B.
Curutchet, Gustavo Andres
Bernik, Delia Leticia
author Fernandez, Marcelo Raul
author_facet Fernandez, Marcelo Raul
Casabona, Maria Guillermina
Anupama, V. N.
Krishnakumar, B.
Curutchet, Gustavo Andres
Bernik, Delia Leticia
author_role author
author2 Casabona, Maria Guillermina
Anupama, V. N.
Krishnakumar, B.
Curutchet, Gustavo Andres
Bernik, Delia Leticia
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Bed Material
Biofilm
Cslm
Pdms
Sem
https://purl.org/becyt/ford/2.8
https://purl.org/becyt/ford/2
topic Bed Material
Biofilm
Cslm
Pdms
Sem
https://purl.org/becyt/ford/2.8
https://purl.org/becyt/ford/2
description The objective of this work is to test the performance of new synthetic polydimethylsiloxane (PDMS)-based bed particles acting as carriers for bacteria biofilms. The particles obtained have a highly interconnected porous structure which offers a large surface adsorption area to the bacteria. In addition, PDMS materials can be cross-linked by copolymerization with other polymers. In the present work we have chosen two hydrophilic polymers: xanthan gum polysaccharide and tetraethoxysilane (TEOS). This versatile composition helps to modulate the interfacial hydrophobic/hydrophilic balance at the particle surface level and the roughness topology and pore size distribution, as revealed by scanning electron microscopy. Biofilm formation of a consortium isolated from a tannery effluent enriched in Sulphate Reducing Bacteria (SRB), and pure Acidithiobacillus ferrooxidans (AF) strains were assayed in three different bed particles synthesized with pure PDMS, PDMS-xanthan gum and PDMS-TEOS hybrids. Bacterial viability assays using confocal laser scanning fluorescence microscopy indicate that inclusion of hydrophilic groups on particle's surface significantly improves both cell adhesion and viability. © 2010 Elsevier B.V.
publishDate 2010
dc.date.none.fl_str_mv 2010-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/71586
Fernandez, Marcelo Raul; Casabona, Maria Guillermina; Anupama, V. N.; Krishnakumar, B.; Curutchet, Gustavo Andres; et al.; PDMS-based porous particles as support beds for cell immobilization: Bacterial biofilm formation as a function of porosity and polymer composition; Elsevier Science; Colloids and Surfaces B: Biointerfaces; 81; 1; 11-2010; 289-296
0927-7765
CONICET Digital
CONICET
url http://hdl.handle.net/11336/71586
identifier_str_mv Fernandez, Marcelo Raul; Casabona, Maria Guillermina; Anupama, V. N.; Krishnakumar, B.; Curutchet, Gustavo Andres; et al.; PDMS-based porous particles as support beds for cell immobilization: Bacterial biofilm formation as a function of porosity and polymer composition; Elsevier Science; Colloids and Surfaces B: Biointerfaces; 81; 1; 11-2010; 289-296
0927-7765
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0927776510003826
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.colsurfb.2010.07.018
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1799196079598600192
spelling PDMS-based porous particles as support beds for cell immobilization: Bacterial biofilm formation as a function of porosity and polymer compositionFernandez, Marcelo RaulCasabona, Maria GuillerminaAnupama, V. N.Krishnakumar, B.Curutchet, Gustavo AndresBernik, Delia LeticiaBed MaterialBiofilmCslmPdmsSemhttps://purl.org/becyt/ford/2.8https://purl.org/becyt/ford/2The objective of this work is to test the performance of new synthetic polydimethylsiloxane (PDMS)-based bed particles acting as carriers for bacteria biofilms. The particles obtained have a highly interconnected porous structure which offers a large surface adsorption area to the bacteria. In addition, PDMS materials can be cross-linked by copolymerization with other polymers. In the present work we have chosen two hydrophilic polymers: xanthan gum polysaccharide and tetraethoxysilane (TEOS). This versatile composition helps to modulate the interfacial hydrophobic/hydrophilic balance at the particle surface level and the roughness topology and pore size distribution, as revealed by scanning electron microscopy. Biofilm formation of a consortium isolated from a tannery effluent enriched in Sulphate Reducing Bacteria (SRB), and pure Acidithiobacillus ferrooxidans (AF) strains were assayed in three different bed particles synthesized with pure PDMS, PDMS-xanthan gum and PDMS-TEOS hybrids. Bacterial viability assays using confocal laser scanning fluorescence microscopy indicate that inclusion of hydrophilic groups on particle's surface significantly improves both cell adhesion and viability. © 2010 Elsevier B.V.Fil: Fernandez, Marcelo Raul. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; ArgentinaFil: Casabona, Maria Guillermina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; ArgentinaFil: Anupama, V. N.. National Institute for Interdisciplinary Science & Technology; IndiaFil: Krishnakumar, B.. National Institute for Interdisciplinary Science & Technology; IndiaFil: Curutchet, Gustavo Andres. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; ArgentinaFil: Bernik, Delia Leticia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; ArgentinaElsevier Science2010-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/71586Fernandez, Marcelo Raul; Casabona, Maria Guillermina; Anupama, V. N.; Krishnakumar, B.; Curutchet, Gustavo Andres; et al.; PDMS-based porous particles as support beds for cell immobilization: Bacterial biofilm formation as a function of porosity and polymer composition; Elsevier Science; Colloids and Surfaces B: Biointerfaces; 81; 1; 11-2010; 289-2960927-7765CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0927776510003826info:eu-repo/semantics/altIdentifier/doi/10.1016/j.colsurfb.2010.07.018info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T14:13:58Zoai:ri.conicet.gov.ar:11336/71586instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 14:13:58.502CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
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