Fluorescence correlation spectroscopy reveals the dynamics of kinesins interacting with organelles during microtubule-dependent transport in cells

Microtubule-dependent motors usually work together to transport organelles through the crowded intracellular milieu. Thus, transport performance depends on how motors organize on the cargo. Unfortunately, the lack of methodologies capable of measuring this organization in cells determines that many...

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Detalles Bibliográficos
Autores: de Rossi, María Cecilia, González Bardeci, Nicolás Diego, Alvarez, Yanina Daniela, Mocksos, Esteban, Romero, Juan José, Bruno, Luciana, Wetzler, Diana Elena, Levi, Valeria
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2019
País:Argentina
Institución:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/123561
Acceso en línea:http://hdl.handle.net/11336/123561
Access Level:acceso abierto
Palabra clave:DROSOPHILA S2 CELLS
INTRACELLULAR TRANSPORT
KINESIN-1
MITOCHONDRIA
MOLECULAR MOTORS
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
id AR_93086b7b63fbb68b79de86bde9fa89fb
oai_identifier_str oai:ri.conicet.gov.ar:11336/123561
network_acronym_str AR
network_name_str Argentina
repository_id_str
dc.title.none.fl_str_mv Fluorescence correlation spectroscopy reveals the dynamics of kinesins interacting with organelles during microtubule-dependent transport in cells
title Fluorescence correlation spectroscopy reveals the dynamics of kinesins interacting with organelles during microtubule-dependent transport in cells
spellingShingle Fluorescence correlation spectroscopy reveals the dynamics of kinesins interacting with organelles during microtubule-dependent transport in cells
de Rossi, María Cecilia
DROSOPHILA S2 CELLS
INTRACELLULAR TRANSPORT
KINESIN-1
MITOCHONDRIA
MOLECULAR MOTORS
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
title_short Fluorescence correlation spectroscopy reveals the dynamics of kinesins interacting with organelles during microtubule-dependent transport in cells
title_full Fluorescence correlation spectroscopy reveals the dynamics of kinesins interacting with organelles during microtubule-dependent transport in cells
title_fullStr Fluorescence correlation spectroscopy reveals the dynamics of kinesins interacting with organelles during microtubule-dependent transport in cells
title_full_unstemmed Fluorescence correlation spectroscopy reveals the dynamics of kinesins interacting with organelles during microtubule-dependent transport in cells
title_sort Fluorescence correlation spectroscopy reveals the dynamics of kinesins interacting with organelles during microtubule-dependent transport in cells
dc.creator.none.fl_str_mv de Rossi, María Cecilia
González Bardeci, Nicolás Diego
Alvarez, Yanina Daniela
Mocksos, Esteban
Romero, Juan José
Bruno, Luciana
Wetzler, Diana Elena
Levi, Valeria
author de Rossi, María Cecilia
author_facet de Rossi, María Cecilia
González Bardeci, Nicolás Diego
Alvarez, Yanina Daniela
Mocksos, Esteban
Romero, Juan José
Bruno, Luciana
Wetzler, Diana Elena
Levi, Valeria
author_role author
author2 González Bardeci, Nicolás Diego
Alvarez, Yanina Daniela
Mocksos, Esteban
Romero, Juan José
Bruno, Luciana
Wetzler, Diana Elena
Levi, Valeria
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv DROSOPHILA S2 CELLS
INTRACELLULAR TRANSPORT
KINESIN-1
MITOCHONDRIA
MOLECULAR MOTORS
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
topic DROSOPHILA S2 CELLS
INTRACELLULAR TRANSPORT
KINESIN-1
MITOCHONDRIA
MOLECULAR MOTORS
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
description Microtubule-dependent motors usually work together to transport organelles through the crowded intracellular milieu. Thus, transport performance depends on how motors organize on the cargo. Unfortunately, the lack of methodologies capable of measuring this organization in cells determines that many aspects of the collective action of motors remain elusive. Here, we combined fluorescence fluctuations and single particle tracking techniques to address how kinesins organize on rod-like mitochondria moving along microtubules in cells. This methodology simultaneously provides mitochondria trajectories and EGFP-tagged kinesin-1 intensity at different mitochondrial positions with millisecond resolution. We show that kinesin exchange at the mitochondrion surface is within ~100 ms and depends on the organelle speed. During anterograde transport, the mitochondrial leading tip presents slower motor exchange in comparison to the rear tip. In contrast, retrograde mitochondria show similar exchange rates of kinesins at both tips. Numerical simulations provide theoretical support to these results and evidence that motors do not share the load equally during intracellular transport.
publishDate 2019
dc.date.none.fl_str_mv 2019-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/123561
de Rossi, María Cecilia; González Bardeci, Nicolás Diego; Alvarez, Yanina Daniela; Mocksos, Esteban; Romero, Juan José; et al.; Fluorescence correlation spectroscopy reveals the dynamics of kinesins interacting with organelles during microtubule-dependent transport in cells; Elsevier Science; Biochimica et Biophysica Acta-Molecular Cell Research; 1867; 1; 10-2019; 1-38
0167-4889
CONICET Digital
CONICET
url http://hdl.handle.net/11336/123561
identifier_str_mv de Rossi, María Cecilia; González Bardeci, Nicolás Diego; Alvarez, Yanina Daniela; Mocksos, Esteban; Romero, Juan José; et al.; Fluorescence correlation spectroscopy reveals the dynamics of kinesins interacting with organelles during microtubule-dependent transport in cells; Elsevier Science; Biochimica et Biophysica Acta-Molecular Cell Research; 1867; 1; 10-2019; 1-38
0167-4889
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0167488919301806
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.bbamcr.2019.118572
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1799195136036438016
spelling Fluorescence correlation spectroscopy reveals the dynamics of kinesins interacting with organelles during microtubule-dependent transport in cellsde Rossi, María CeciliaGonzález Bardeci, Nicolás DiegoAlvarez, Yanina DanielaMocksos, EstebanRomero, Juan JoséBruno, LucianaWetzler, Diana ElenaLevi, ValeriaDROSOPHILA S2 CELLSINTRACELLULAR TRANSPORTKINESIN-1MITOCHONDRIAMOLECULAR MOTORShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Microtubule-dependent motors usually work together to transport organelles through the crowded intracellular milieu. Thus, transport performance depends on how motors organize on the cargo. Unfortunately, the lack of methodologies capable of measuring this organization in cells determines that many aspects of the collective action of motors remain elusive. Here, we combined fluorescence fluctuations and single particle tracking techniques to address how kinesins organize on rod-like mitochondria moving along microtubules in cells. This methodology simultaneously provides mitochondria trajectories and EGFP-tagged kinesin-1 intensity at different mitochondrial positions with millisecond resolution. We show that kinesin exchange at the mitochondrion surface is within ~100 ms and depends on the organelle speed. During anterograde transport, the mitochondrial leading tip presents slower motor exchange in comparison to the rear tip. In contrast, retrograde mitochondria show similar exchange rates of kinesins at both tips. Numerical simulations provide theoretical support to these results and evidence that motors do not share the load equally during intracellular transport.Fil: de Rossi, María Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; ArgentinaFil: González Bardeci, Nicolás Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Alvarez, Yanina Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Mocksos, Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Simulación Computacional para Aplicaciones Tecnológicas; ArgentinaFil: Romero, Juan José. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Bruno, Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Física de Buenos Aires; ArgentinaFil: Wetzler, Diana Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Levi, Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; ArgentinaElsevier Science2019-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/123561de Rossi, María Cecilia; González Bardeci, Nicolás Diego; Alvarez, Yanina Daniela; Mocksos, Esteban; Romero, Juan José; et al.; Fluorescence correlation spectroscopy reveals the dynamics of kinesins interacting with organelles during microtubule-dependent transport in cells; Elsevier Science; Biochimica et Biophysica Acta-Molecular Cell Research; 1867; 1; 10-2019; 1-380167-4889CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0167488919301806info:eu-repo/semantics/altIdentifier/doi/10.1016/j.bbamcr.2019.118572info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T13:44:20Zoai:ri.conicet.gov.ar:11336/123561instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 13:44:20.605CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
score 15,81155

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