Neotropical termite microbiomes as sources of novel plant cell wall degrading enzymes

Fil: Romero Victorica, Matías. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO). Hurlingham, Buenos Aires, Argentina.

Detalles Bibliográficos
Autores: Romero Victorica, Matías, Soria, Marcelo Abel, Batista García, Ramón Alberto, Ceja Navarro, Javier A., Vikram, Surendra, Ortiz, Maximiliano, Ontañon, Ornella, Ghio, Silvina
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2020
País:Argentina
Institución:Universidad de Buenos Aires. Facultad de Agronomía
Repositorio:FAUBA Digital (UBA-FAUBA)
Idioma:inglés
OAI Identifier:snrd:2020romerovictorica
Acceso en línea:http://ri.agro.uba.ar/greenstone3/library/collection/arti/document/2020romerovictorica
Access Level:acceso abierto
Palabra clave:METAGENÓMICA
SECUENCIACIÓN DE PRÓXIMA GENERACIÓN
id AR_927e87ff7af2a20005a2a5e278946f97
oai_identifier_str snrd:2020romerovictorica
network_acronym_str AR
network_name_str Argentina
repository_id_str
dc.title.none.fl_str_mv Neotropical termite microbiomes as sources of novel plant cell wall degrading enzymes
title Neotropical termite microbiomes as sources of novel plant cell wall degrading enzymes
spellingShingle Neotropical termite microbiomes as sources of novel plant cell wall degrading enzymes
Romero Victorica, Matías
METAGENÓMICA
SECUENCIACIÓN DE PRÓXIMA GENERACIÓN
title_short Neotropical termite microbiomes as sources of novel plant cell wall degrading enzymes
title_full Neotropical termite microbiomes as sources of novel plant cell wall degrading enzymes
title_fullStr Neotropical termite microbiomes as sources of novel plant cell wall degrading enzymes
title_full_unstemmed Neotropical termite microbiomes as sources of novel plant cell wall degrading enzymes
title_sort Neotropical termite microbiomes as sources of novel plant cell wall degrading enzymes
dc.creator.none.fl_str_mv Romero Victorica, Matías
Soria, Marcelo Abel
Batista García, Ramón Alberto
Ceja Navarro, Javier A.
Vikram, Surendra
Ortiz, Maximiliano
Ontañon, Ornella
Ghio, Silvina
author Romero Victorica, Matías
author_facet Romero Victorica, Matías
Soria, Marcelo Abel
Batista García, Ramón Alberto
Ceja Navarro, Javier A.
Vikram, Surendra
Ortiz, Maximiliano
Ontañon, Ornella
Ghio, Silvina
author_role author
author2 Soria, Marcelo Abel
Batista García, Ramón Alberto
Ceja Navarro, Javier A.
Vikram, Surendra
Ortiz, Maximiliano
Ontañon, Ornella
Ghio, Silvina
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv METAGENÓMICA
SECUENCIACIÓN DE PRÓXIMA GENERACIÓN
topic METAGENÓMICA
SECUENCIACIÓN DE PRÓXIMA GENERACIÓN
description Fil: Romero Victorica, Matías. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO). Hurlingham, Buenos Aires, Argentina.
publishDate 2020
dc.date.none.fl_str_mv 2020
dc.type.none.fl_str_mv article
info:eu-repo/semantics/article
publishedVersion
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv 10.1038/s41598-020-60850-5
2045-2322
http://ri.agro.uba.ar/greenstone3/library/collection/arti/document/2020romerovictorica
identifier_str_mv 10.1038/s41598-020-60850-5
2045-2322
url http://ri.agro.uba.ar/greenstone3/library/collection/arti/document/2020romerovictorica
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
openAccess
http://ri.agro.uba.ar/greenstone3/library/page/biblioteca#section4
eu_rights_str_mv openAccess
rights_invalid_str_mv openAccess
http://ri.agro.uba.ar/greenstone3/library/page/biblioteca#section4
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Scientific Reports
Vol.10, no.3864
14
https://www.nature.com
reponame:FAUBA Digital (UBA-FAUBA)
instname:Universidad de Buenos Aires. Facultad de Agronomía
instname_str Universidad de Buenos Aires. Facultad de Agronomía
reponame_str FAUBA Digital (UBA-FAUBA)
collection FAUBA Digital (UBA-FAUBA)
repository.name.fl_str_mv FAUBA Digital (UBA-FAUBA) - Universidad de Buenos Aires. Facultad de Agronomía
repository.mail.fl_str_mv martino@agro.uba.ar;berasa@agro.uba.ar
_version_ 1799196711569063936
spelling Neotropical termite microbiomes as sources of novel plant cell wall degrading enzymesRomero Victorica, MatíasSoria, Marcelo AbelBatista García, Ramón AlbertoCeja Navarro, Javier A.Vikram, SurendraOrtiz, MaximilianoOntañon, OrnellaGhio, SilvinaMETAGENÓMICASECUENCIACIÓN DE PRÓXIMA GENERACIÓNFil: Romero Victorica, Matías. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO). Hurlingham, Buenos Aires, Argentina.Fil: Soria, Marcelo Abel. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Biología Aplicada y Alimentos. Cátedra de Microbiología Agrícola. Buenos Aires, Argentina.Fil: Batista García, Ramón Alberto. Universidad Autónoma del Estado Morelos. Instituto de Investigación en Ciencias Básicas y Aplicadas. Centro de Investigación en Dinámica Celular. Cuernavaca, Morelos, Mexico.Fil: Ceja Navarro, Javier A. Biological Systems and Engineering Division. Lawrence Berkeley National Laboratory. Berkeley, California, USA.Fil: Vikram, Surendra. Department Biochemistry, Genetics and Microbiology. Centre for Microbial Ecology and Genomics. University of Pretoria. Pretoria, South Africa.Fil: Ortiz, Maximiliano.University of Pretoria. Centre for Microbial Ecology and Genomics. Genetics and Microbiology. Pretoria, South Africa.Fil: Ontañon, Ornella. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO). Hurlingham, Buenos Aires, Argentina.Fil: Ghio, Silvina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO). Hurlingham, Buenos Aires, Argentina.In this study, we used shotgun metagenomic sequencing to characterise the microbial metabolic potential for lignocellulose transformation in the gut of two colonies of Argentine higher termite species with diferent feeding habits, Cortaritermes fulviceps and Nasutitermes aquilinus. Our goal was to assess the microbial community compositions and metabolic capacity, and to identify genes involved in lignocellulose degradation. Individuals from both termite species contained the same fve dominant bacterial phyla (Spirochaetes, Firmicutes, Proteobacteria, Fibrobacteres and Bacteroidetes) although with diferent relative abundances. However, detected functional capacity varied, with C. fulviceps (a grass-wood-feeder) gut microbiome samples containing more genes related to amino acid metabolism, whereas N. aquilinus (a wood-feeder) gut microbiome samples were enriched in genes involved in carbohydrate metabolism and cellulose degradation. The C. fulviceps gut microbiome was enriched specifcally in genes coding for debranching- and oligosaccharide-degrading enzymes. These fndings suggest an association between the primary food source and the predicted categories of the enzymes present in the gut microbiomes of each species. To further investigate the termite microbiomes as sources of biotechnologically relevant glycosyl hydrolases, a putative GH10 endo-β-1,4- xylanase, Xyl10E, was cloned and expressed in Escherichia coli. Functional analysis of the recombinant metagenome-derived enzyme showed high specifcity towards beechwood xylan (288.1 IU/mg), with the optimum activity at 50°C and a pH-activity range from 5 to 10. These characteristics suggest that Xy110E may be a promising candidate for further development in lignocellulose deconstruction applications.tbls., grafs., il., fot.2020articleinfo:eu-repo/semantics/articlepublishedVersioninfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf10.1038/s41598-020-60850-52045-2322http://ri.agro.uba.ar/greenstone3/library/collection/arti/document/2020romerovictoricaScientific ReportsVol.10, no.386414https://www.nature.comreponame:FAUBA Digital (UBA-FAUBA)instname:Universidad de Buenos Aires. Facultad de Agronomíaenginfo:eu-repo/semantics/openAccessopenAccesshttp://ri.agro.uba.ar/greenstone3/library/page/biblioteca#section42024-05-10T10:43:18Zsnrd:2020romerovictoricainstacron:UBA-FAUBAInstitucionalhttp://ri.agro.uba.ar/Universidad públicaNo correspondehttp://ri.agro.uba.ar/greenstone3/oaiserver?verb=ListSetsmartino@agro.uba.ar;berasa@agro.uba.ar ArgentinaNo correspondeNo correspondeNo correspondeopendoar:27292024-05-10 10:43:19.548FAUBA Digital (UBA-FAUBA) - Universidad de Buenos Aires. Facultad de Agronomíafalse
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