Role of bacterial pyrroloquinoline quinone in phosphate solubilizing ability and in plant growth promotion on strain <i>Serratia</i> sp. S119
The aim of this study was to analyze if cofactor pyrroquinoline quinone from <i>Serratia</i> sp. S119 is involved in the inorganic phosphate solubilization mechanism and in its ability to promote the plant growth. Site directed mutagenesis was performed to obtain a pqqE- minus mutant of...
| Autores: | , , , , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2017 |
| País: | Argentina |
| Institución: | Universidad Nacional de La Plata |
| Repositorio: | SEDICI (UNLP) |
| Idioma: | inglés |
| OAI Identifier: | oai:sedici.unlp.edu.ar:10915/132692 |
| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/132692 |
| Access Level: | acceso abierto |
| Palabra clave: | Ciencias Exactas Química PQQ cofactor Serratia sp. Phosphate solubilization Plant growth promotion pqqE sequence |
| Sumario: | The aim of this study was to analyze if cofactor pyrroquinoline quinone from <i>Serratia</i> sp. S119 is involved in the inorganic phosphate solubilization mechanism and in its ability to promote the plant growth. Site directed mutagenesis was performed to obtain a pqqE- minus mutant of strain <i>Serratia</i> sp. S119. The phosphate solubilization ability, gluconate and PQQ production of the mutant <i>Serratia</i> sp. RSL (pqqE-) was analyzed. Mutant RSL (pqqE-) showed significant decrease in P soluble and gluconic acid levels produced and undetectable levels of PQQ cofactor compared with wild-type strain. Complementation with synthetic PQQ cofactor restored P solubilization and gluconate production reaching the levels produced by wild-type strain. PqqE gene sequence indicated that it is highly conserved within <i>Serratia</i> strains and its product shows conserved motifs found in other PqqE proteins of several bacteria. The effect of the inoculation of the PQQ- mutant on peanut and maize plants was evaluated in pot assays. Plants growth parameters showed no differences among the different treatments indicating that PQQ from <i>Serratia</i> sp. S119 is not involved in the growth promotion of these plants. PQQ cofactor is essential for phosphate solubilization ability of <i>Serratia</i> sp. S119 but is not required for growth promotion of peanut and maize plants. |
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