Purification of two bacteriocins produced by Enterococcus faecalis DBFIQ E24 strain isolated from raw bovine milk
Enterococcus faecalis DBFIQ E24 was formerly characterised as a producer of antibacterial and antifungal substances. This strain is vancomycin sensitive, nonhaemolytic and gelatinase negative. SDS-PAGE revealed the presence of an active band located between 1060 and 3500 Da. After the application of...
| Autores: | , , , |
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| Tipo de documento: | artigo |
| Estado: | Versão publicada |
| Data de publicação: | 2016 |
| País: | Argentina |
| Recursos: | Consejo Nacional de Investigaciones Científicas y Técnicas |
| Repositório: | CONICET Digital (CONICET) |
| Idioma: | inglês |
| OAI Identifier: | oai:ri.conicet.gov.ar:11336/23812 |
| Acesso em linha: | http://hdl.handle.net/11336/23812 |
| Access Level: | Acceso aberto |
| Palavra-chave: | Enterococcus Faecalis Strain Enterocin Purification Food Biopreservative Wide Inhibitory Spectra https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
| Resumo: | Enterococcus faecalis DBFIQ E24 was formerly characterised as a producer of antibacterial and antifungal substances. This strain is vancomycin sensitive, nonhaemolytic and gelatinase negative. SDS-PAGE revealed the presence of an active band located between 1060 and 3500 Da. After the application of a three-step purification procedure, two antimicrobial peptides were isolated. One of them is mostly active against Bacillus cereus with a molecular mass of 1364 Da, and the other one with a molecular mass of 1686 Da is mainly active against Escherichia coli. These results confirm the technological potential of E. faecalis DBFIQ E24 strain as a GRAS food biopreservative. |
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