Duodenal intraepithelial lymphocytes of children with cow milk allergy preferentially bind the glycan-binding protein galectin-3

A breakdown in intestinal homeostasis results in inflammatory bowel diseases including coeliac disease and allergy. Galectins, evolutionarily conserved beta-galactoside-binding proteins, can modulate immune-epithelial cell interactions by influencing immune cell fate and cytokine secretion. In this...

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Autores: Mercer, Natalia, Guzman, Luciana, Cueto Rua, Eduardo, Drut, Ricardo, Ahmed, H., Vasta, G. R., Toscano, Marta Alicia, Rabinovich, Gabriel Adrián, Docena, Guillermo Horacio
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2009
País:Argentina
Institución:Universidad Nacional de La Plata
Repositorio:SEDICI (UNLP)
Idioma:inglés
OAI Identifier:oai:sedici.unlp.edu.ar:10915/101008
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/101008
Access Level:acceso abierto
Palabra clave:Bioquímica y biología molecular
Medicina básica
Ciencias médicas y de la salud
Gut
Lymphocytes
Galectin-3
Allergy
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spelling Duodenal intraepithelial lymphocytes of children with cow milk allergy preferentially bind the glycan-binding protein galectin-3Mercer, NataliaGuzman, LucianaCueto Rua, EduardoDrut, RicardoAhmed, H.Vasta, G. R.Toscano, Marta AliciaRabinovich, Gabriel AdriánDocena, Guillermo HoracioBioquímica y biología molecularMedicina básicaCiencias médicas y de la saludGutLymphocytesGalectin-3AllergyA breakdown in intestinal homeostasis results in inflammatory bowel diseases including coeliac disease and allergy. Galectins, evolutionarily conserved beta-galactoside-binding proteins, can modulate immune-epithelial cell interactions by influencing immune cell fate and cytokine secretion. In this study we investigated the glycosylation signature, as well as the regulated expression of galectin-1 and -3 in human duodenal samples of allergic and non-allergic children. Whereas galectin-1 was predominantly localized in the epithelial compartment (epithelial cells and intraepithelial lymphocytes) and the underlying lamina propria (T cells, macrophages and plasma cells), galectin-3 was mainly expressed by crypt epithelial cells and macrophages in the lamina propria. Remarkably, expression of these galectins was not significantly altered in allergic versus non-allergic patients. Investigation of the glycophenotype of the duodenal inflammatory microenvironment revealed substantial alpha2-6-linked sialic acid bound to galactose in lamina propria plasma cells, macrophages and intraepithelial lymphocytes and significant levels of asialo core 1 O-glycans in CD68+ macrophages and enterocytes. Galectin-1 preferentially bound to neutrophils, plasma cells and enterocytes, while galectin-3 binding sites were mainly distributed on macrophages and intraepithelial lymphocytes. Notably, galectin-3, but not galectin-1 binding, was substantially increased in intraepithelial gut lymphocytes of allergic patients compared to non-allergic subjects, suggesting a potential role of galectin-3-glycan interactions in shaping epithelial-immune cell connections during allergic inflammatory processes.Laboratorio de Investigaciones del Sistema Inmune2009info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf207-217http://sedici.unlp.edu.ar/handle/10915/101008enginfo:eu-repo/semantics/altIdentifier/url/https://ri.conicet.gov.ar/11336/25863info:eu-repo/semantics/altIdentifier/url/http://journals.sagepub.com/doi/abs/10.1177/039463200902200123info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3844523/info:eu-repo/semantics/altIdentifier/url/http://journals.sagepub.com/doi/abs/10.1177/039463200902200123info:eu-repo/semantics/altIdentifier/hdl/11336/25863info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2024-05-08T12:57:57Zoai:sedici.unlp.edu.ar:10915/101008Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292024-05-08 12:57:57.896SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Duodenal intraepithelial lymphocytes of children with cow milk allergy preferentially bind the glycan-binding protein galectin-3
title Duodenal intraepithelial lymphocytes of children with cow milk allergy preferentially bind the glycan-binding protein galectin-3
spellingShingle Duodenal intraepithelial lymphocytes of children with cow milk allergy preferentially bind the glycan-binding protein galectin-3
Mercer, Natalia
Bioquímica y biología molecular
Medicina básica
Ciencias médicas y de la salud
Gut
Lymphocytes
Galectin-3
Allergy
title_short Duodenal intraepithelial lymphocytes of children with cow milk allergy preferentially bind the glycan-binding protein galectin-3
title_full Duodenal intraepithelial lymphocytes of children with cow milk allergy preferentially bind the glycan-binding protein galectin-3
title_fullStr Duodenal intraepithelial lymphocytes of children with cow milk allergy preferentially bind the glycan-binding protein galectin-3
title_full_unstemmed Duodenal intraepithelial lymphocytes of children with cow milk allergy preferentially bind the glycan-binding protein galectin-3
title_sort Duodenal intraepithelial lymphocytes of children with cow milk allergy preferentially bind the glycan-binding protein galectin-3
dc.creator.none.fl_str_mv Mercer, Natalia
Guzman, Luciana
Cueto Rua, Eduardo
Drut, Ricardo
Ahmed, H.
Vasta, G. R.
Toscano, Marta Alicia
Rabinovich, Gabriel Adrián
Docena, Guillermo Horacio
author Mercer, Natalia
author_facet Mercer, Natalia
Guzman, Luciana
Cueto Rua, Eduardo
Drut, Ricardo
Ahmed, H.
Vasta, G. R.
Toscano, Marta Alicia
Rabinovich, Gabriel Adrián
Docena, Guillermo Horacio
author_role author
author2 Guzman, Luciana
Cueto Rua, Eduardo
Drut, Ricardo
Ahmed, H.
Vasta, G. R.
Toscano, Marta Alicia
Rabinovich, Gabriel Adrián
Docena, Guillermo Horacio
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Bioquímica y biología molecular
Medicina básica
Ciencias médicas y de la salud
Gut
Lymphocytes
Galectin-3
Allergy
topic Bioquímica y biología molecular
Medicina básica
Ciencias médicas y de la salud
Gut
Lymphocytes
Galectin-3
Allergy
description A breakdown in intestinal homeostasis results in inflammatory bowel diseases including coeliac disease and allergy. Galectins, evolutionarily conserved beta-galactoside-binding proteins, can modulate immune-epithelial cell interactions by influencing immune cell fate and cytokine secretion. In this study we investigated the glycosylation signature, as well as the regulated expression of galectin-1 and -3 in human duodenal samples of allergic and non-allergic children. Whereas galectin-1 was predominantly localized in the epithelial compartment (epithelial cells and intraepithelial lymphocytes) and the underlying lamina propria (T cells, macrophages and plasma cells), galectin-3 was mainly expressed by crypt epithelial cells and macrophages in the lamina propria. Remarkably, expression of these galectins was not significantly altered in allergic versus non-allergic patients. Investigation of the glycophenotype of the duodenal inflammatory microenvironment revealed substantial alpha2-6-linked sialic acid bound to galactose in lamina propria plasma cells, macrophages and intraepithelial lymphocytes and significant levels of asialo core 1 O-glycans in CD68+ macrophages and enterocytes. Galectin-1 preferentially bound to neutrophils, plasma cells and enterocytes, while galectin-3 binding sites were mainly distributed on macrophages and intraepithelial lymphocytes. Notably, galectin-3, but not galectin-1 binding, was substantially increased in intraepithelial gut lymphocytes of allergic patients compared to non-allergic subjects, suggesting a potential role of galectin-3-glycan interactions in shaping epithelial-immune cell connections during allergic inflammatory processes.
publishDate 2009
dc.date.none.fl_str_mv 2009
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/101008
url http://sedici.unlp.edu.ar/handle/10915/101008
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://ri.conicet.gov.ar/11336/25863
info:eu-repo/semantics/altIdentifier/url/http://journals.sagepub.com/doi/abs/10.1177/039463200902200123
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3844523/
info:eu-repo/semantics/altIdentifier/url/http://journals.sagepub.com/doi/abs/10.1177/039463200902200123
info:eu-repo/semantics/altIdentifier/hdl/11336/25863
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
207-217
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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