Combined use of electron and light microscopy techniques reveals false secondary shell units in Megaloolithidae eggshells

Abnormalities in the histo- and ultrastructure of the amniote eggshell are often related to diverse factors, such as ambient stress during egg formation, pathologies altering the physiology of the egg-laying females, or evolutionarily selected modifications of the eggshell structure that vary the ph...

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Detalles Bibliográficos
Autores: Moreno Azanza, Miguel, Bauluz, Blanca, Canudo, José Ignacio, Gasca Pérez, José Manuel, Torcida Fernández Baldor, Fidel
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2016
País:Argentina
Institución:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/62347
Acceso en línea:http://hdl.handle.net/11336/62347
Access Level:acceso abierto
Palabra clave:Megaloolithidae
Taphonomic
Fossilization
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spelling Combined use of electron and light microscopy techniques reveals false secondary shell units in Megaloolithidae eggshellsMoreno Azanza, MiguelBauluz, BlancaCanudo, José IgnacioGasca Pérez, José ManuelTorcida Fernández Baldor, FidelMegaloolithidaeTaphonomicFossilizationAbnormalities in the histo- and ultrastructure of the amniote eggshell are often related to diverse factors, such as ambient stress during egg formation, pathologies altering the physiology of the egg-laying females, or evolutionarily selected modifications of the eggshell structure that vary the physical properties of the egg, for example increasing its strength so as to avoid fracture during incubation. When dealing with fossil materials, all the above hypotheses are plausible, but a detailed taphonomical study has to be performed to rule out the possibility that secondary processes of recrystallization have occurred during fossilization. Traditional analyses, such as optical microscopy inspection and cathodoluminescence, have proven not to be enough to understand the taphonomic story of some eggshells. Recently, electron backscatter diffraction has been used, in combination with other techniques, to better understand the alteration of fossil eggshells. Here we present a combined study using scanning electron microscopy, optical microscopy, cathodoluminescence and electron backscatter diffraction of eggshell fragments assigned to Megaloolithus cf. siruguei from the Upper Cretaceous outcrops of the Cameros Basin. We focus our study on the presence of secondary shell units that mimic most aspects of the ultrastructure of the eggshell mammillae, but grow far from the inner surface of the eggshell. We call these structures extra-spherulites, describe their crystal structure and demonstrate their secondary origin. Our study has important implications for the interpretation of secondary shell units as biological or pathological structures. Thus, electron backscatter diffraction complements other microscope techniques as a useful tool for understanding taphonomical alterations in fossil eggshells.Fil: Moreno Azanza, Miguel. Universidad de Zaragoza; EspañaFil: Bauluz, Blanca. Universidad de Zaragoza; EspañaFil: Canudo, José Ignacio. Universidad de Zaragoza; EspañaFil: Gasca Pérez, José Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte; Argentina. Provincia de Neuquén. Ministerio de Energía, Ambiente y Servicios Públicos. Dirección Provincial de Minería. Museo Provincial de Ciencias Naturales Prof. ; Argentina. Universidad de Zaragoza; EspañaFil: Torcida Fernández Baldor, Fidel. Museo de Los Dinosaurios, Salas de Los Infantes, Burgos; EspañaPublic Library of Science2016-05-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/62347Moreno Azanza, Miguel; Bauluz, Blanca; Canudo, José Ignacio; Gasca Pérez, José Manuel; Torcida Fernández Baldor, Fidel; Combined use of electron and light microscopy techniques reveals false secondary shell units in Megaloolithidae eggshells; Public Library of Science; Plos One; 11; 5; 4-5-2016; 1-171932-6203CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0153026info:eu-repo/semantics/altIdentifier/url/https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0153026info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T14:27:53Zoai:ri.conicet.gov.ar:11336/62347instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 14:27:53.836CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Combined use of electron and light microscopy techniques reveals false secondary shell units in Megaloolithidae eggshells
title Combined use of electron and light microscopy techniques reveals false secondary shell units in Megaloolithidae eggshells
spellingShingle Combined use of electron and light microscopy techniques reveals false secondary shell units in Megaloolithidae eggshells
Moreno Azanza, Miguel
Megaloolithidae
Taphonomic
Fossilization
title_short Combined use of electron and light microscopy techniques reveals false secondary shell units in Megaloolithidae eggshells
title_full Combined use of electron and light microscopy techniques reveals false secondary shell units in Megaloolithidae eggshells
title_fullStr Combined use of electron and light microscopy techniques reveals false secondary shell units in Megaloolithidae eggshells
title_full_unstemmed Combined use of electron and light microscopy techniques reveals false secondary shell units in Megaloolithidae eggshells
title_sort Combined use of electron and light microscopy techniques reveals false secondary shell units in Megaloolithidae eggshells
dc.creator.none.fl_str_mv Moreno Azanza, Miguel
Bauluz, Blanca
Canudo, José Ignacio
Gasca Pérez, José Manuel
Torcida Fernández Baldor, Fidel
author Moreno Azanza, Miguel
author_facet Moreno Azanza, Miguel
Bauluz, Blanca
Canudo, José Ignacio
Gasca Pérez, José Manuel
Torcida Fernández Baldor, Fidel
author_role author
author2 Bauluz, Blanca
Canudo, José Ignacio
Gasca Pérez, José Manuel
Torcida Fernández Baldor, Fidel
author2_role author
author
author
author
dc.subject.none.fl_str_mv Megaloolithidae
Taphonomic
Fossilization
topic Megaloolithidae
Taphonomic
Fossilization
description Abnormalities in the histo- and ultrastructure of the amniote eggshell are often related to diverse factors, such as ambient stress during egg formation, pathologies altering the physiology of the egg-laying females, or evolutionarily selected modifications of the eggshell structure that vary the physical properties of the egg, for example increasing its strength so as to avoid fracture during incubation. When dealing with fossil materials, all the above hypotheses are plausible, but a detailed taphonomical study has to be performed to rule out the possibility that secondary processes of recrystallization have occurred during fossilization. Traditional analyses, such as optical microscopy inspection and cathodoluminescence, have proven not to be enough to understand the taphonomic story of some eggshells. Recently, electron backscatter diffraction has been used, in combination with other techniques, to better understand the alteration of fossil eggshells. Here we present a combined study using scanning electron microscopy, optical microscopy, cathodoluminescence and electron backscatter diffraction of eggshell fragments assigned to Megaloolithus cf. siruguei from the Upper Cretaceous outcrops of the Cameros Basin. We focus our study on the presence of secondary shell units that mimic most aspects of the ultrastructure of the eggshell mammillae, but grow far from the inner surface of the eggshell. We call these structures extra-spherulites, describe their crystal structure and demonstrate their secondary origin. Our study has important implications for the interpretation of secondary shell units as biological or pathological structures. Thus, electron backscatter diffraction complements other microscope techniques as a useful tool for understanding taphonomical alterations in fossil eggshells.
publishDate 2016
dc.date.none.fl_str_mv 2016-05-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/62347
Moreno Azanza, Miguel; Bauluz, Blanca; Canudo, José Ignacio; Gasca Pérez, José Manuel; Torcida Fernández Baldor, Fidel; Combined use of electron and light microscopy techniques reveals false secondary shell units in Megaloolithidae eggshells; Public Library of Science; Plos One; 11; 5; 4-5-2016; 1-17
1932-6203
CONICET Digital
CONICET
url http://hdl.handle.net/11336/62347
identifier_str_mv Moreno Azanza, Miguel; Bauluz, Blanca; Canudo, José Ignacio; Gasca Pérez, José Manuel; Torcida Fernández Baldor, Fidel; Combined use of electron and light microscopy techniques reveals false secondary shell units in Megaloolithidae eggshells; Public Library of Science; Plos One; 11; 5; 4-5-2016; 1-17
1932-6203
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0153026
info:eu-repo/semantics/altIdentifier/url/https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0153026
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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