FTIR and Raman analysis of l -cysteine ethyl ester HCl interaction with dipalmitoylphosphatidylcholine in anhydrous and hydrated states

Interactions of l‐cysteine ethyl ester hydrochloride (CE), a bioactive cysteine derivative, with dipalmitoylphosphatidylcholine (DPPC) were investigated. To gain a deeper insight into analyzing l‐cysteine ethyl ester HCl interaction with liposomes of DPPC in anhydrous and hydrated states, we perform...

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Bibliographic Details
Authors: Arias, Juan Marcelo, Tuttolomondo, María Eugenia, Díaz, Sonia Beatriz, Ben Altabef, Aída
Format: article
Status:Published version
Publication Date:2015
Country:Argentina
Institution:Consejo Nacional de Investigaciones Científicas y Técnicas
Repository:CONICET Digital (CONICET)
Language:English
OAI Identifier:oai:ri.conicet.gov.ar:11336/43189
Online Access:http://hdl.handle.net/11336/43189
Access Level:Open access
Keyword:Liposome
Raman
Ftir
Interaction
https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
Description
Summary:Interactions of l‐cysteine ethyl ester hydrochloride (CE), a bioactive cysteine derivative, with dipalmitoylphosphatidylcholine (DPPC) were investigated. To gain a deeper insight into analyzing l‐cysteine ethyl ester HCl interaction with liposomes of DPPC in anhydrous and hydrated states, we performed experimental studies by infrared (Fourier transform infrared spectroscopy) and Raman spectroscopies. The results revealed that the interaction of CE with the phospholipid head groups was the same in absence or presence of water. In both states, the wavenumber of the PO2− group and CN bond of the choline group decreased. This behavior can be ascribed to the replacement of hydration water and binding to the phosphate group. In the Raman spectrum results for the anhydrous and gel states, the SH stretching band of the CE shifted to lower frequencies with a decrease in its force constant. Biologically active lipophilic molecules such as CE should be studied in terms of their interaction with lipid bilayers prior to the development of advanced lipid carrier systems such as liposomes. The results of these studies provide information on membrane integrity and physicochemical properties that are essential for the rational design of lipidic drug delivery systems.

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