Fluorescent ratiometric MFC probe sensitive to early stages of α-Synuclein aggregation

We introduce a sensor molecule, AS140-MFC, consisting of a covalent adduct of an Ala-to-Cys mutant of α-synuclein with the 3-hydroxychromone dual emission dye MFC. We show that the AS140-MFC construct is a multiparametric fluorescent probe suitable for the continuous monitoring of protein aggregatio...

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Detalhes bibliográficos
Autores: Yushchenko, Dmytro A., Fauerbach, Jonathan Arturo, Thirunavukkuarasu, Shyamala, Jares, Elizabeth Andrea, Jovin, Thomas M.
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2010
País:Argentina
Recursos:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/68821
Acesso em linha:http://hdl.handle.net/11336/68821
Access Level:acceso abierto
Palavra-chave:Polarity Sensitive Dyes
Amyloid
Synuclein
https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
Descrição
Resumo:We introduce a sensor molecule, AS140-MFC, consisting of a covalent adduct of an Ala-to-Cys mutant of α-synuclein with the 3-hydroxychromone dual emission dye MFC. We show that the AS140-MFC construct is a multiparametric fluorescent probe suitable for the continuous monitoring of protein aggregation and is sensitive to the early and intermediate stages of α-synuclein aggregation, a process associated with Parkinsons disease.

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