Fluorescent ratiometric MFC probe sensitive to early stages of α-Synuclein aggregation

We introduce a sensor molecule, AS140-MFC, consisting of a covalent adduct of an Ala-to-Cys mutant of α-synuclein with the 3-hydroxychromone dual emission dye MFC. We show that the AS140-MFC construct is a multiparametric fluorescent probe suitable for the continuous monitoring of protein aggregatio...

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Detalles Bibliográficos
Autores: Yushchenko, Dmytro A., Fauerbach, Jonathan Arturo, Thirunavukkuarasu, Shyamala, Jares, Elizabeth Andrea, Jovin, Thomas M.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2010
País:Argentina
Institución:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/68821
Acceso en línea:http://hdl.handle.net/11336/68821
Access Level:acceso abierto
Palabra clave:Polarity Sensitive Dyes
Amyloid
Synuclein
https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
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repository_id_str
spelling Fluorescent ratiometric MFC probe sensitive to early stages of α-Synuclein aggregationYushchenko, Dmytro A.Fauerbach, Jonathan ArturoThirunavukkuarasu, ShyamalaJares, Elizabeth AndreaJovin, Thomas M.Polarity Sensitive DyesAmyloidSynucleinhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1We introduce a sensor molecule, AS140-MFC, consisting of a covalent adduct of an Ala-to-Cys mutant of α-synuclein with the 3-hydroxychromone dual emission dye MFC. We show that the AS140-MFC construct is a multiparametric fluorescent probe suitable for the continuous monitoring of protein aggregation and is sensitive to the early and intermediate stages of α-synuclein aggregation, a process associated with Parkinsons disease.Fil: Yushchenko, Dmytro A.. Max Planck Institute for Biophysical Chemistry; AlemaniaFil: Fauerbach, Jonathan Arturo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; ArgentinaFil: Thirunavukkuarasu, Shyamala. Max Planck Institute for Biophysical Chemistry; AlemaniaFil: Jares, Elizabeth Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; ArgentinaFil: Jovin, Thomas M.. Max Planck Institute for Biophysical Chemistry; AlemaniaAmerican Chemical Society2010-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/68821Yushchenko, Dmytro A.; Fauerbach, Jonathan Arturo; Thirunavukkuarasu, Shyamala; Jares, Elizabeth Andrea; Jovin, Thomas M.; Fluorescent ratiometric MFC probe sensitive to early stages of α-Synuclein aggregation; American Chemical Society; Journal of the American Chemical Society; 132; 23; 6-2010; 7860-78610002-7863CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1021/ja102838ninfo:eu-repo/semantics/altIdentifier/url/https://pubs.acs.org/doi/abs/10.1021/ja102838ninfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T13:33:25Zoai:ri.conicet.gov.ar:11336/68821instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 13:33:26.128CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Fluorescent ratiometric MFC probe sensitive to early stages of α-Synuclein aggregation
title Fluorescent ratiometric MFC probe sensitive to early stages of α-Synuclein aggregation
spellingShingle Fluorescent ratiometric MFC probe sensitive to early stages of α-Synuclein aggregation
Yushchenko, Dmytro A.
Polarity Sensitive Dyes
Amyloid
Synuclein
https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
title_short Fluorescent ratiometric MFC probe sensitive to early stages of α-Synuclein aggregation
title_full Fluorescent ratiometric MFC probe sensitive to early stages of α-Synuclein aggregation
title_fullStr Fluorescent ratiometric MFC probe sensitive to early stages of α-Synuclein aggregation
title_full_unstemmed Fluorescent ratiometric MFC probe sensitive to early stages of α-Synuclein aggregation
title_sort Fluorescent ratiometric MFC probe sensitive to early stages of α-Synuclein aggregation
dc.creator.none.fl_str_mv Yushchenko, Dmytro A.
Fauerbach, Jonathan Arturo
Thirunavukkuarasu, Shyamala
Jares, Elizabeth Andrea
Jovin, Thomas M.
author Yushchenko, Dmytro A.
author_facet Yushchenko, Dmytro A.
Fauerbach, Jonathan Arturo
Thirunavukkuarasu, Shyamala
Jares, Elizabeth Andrea
Jovin, Thomas M.
author_role author
author2 Fauerbach, Jonathan Arturo
Thirunavukkuarasu, Shyamala
Jares, Elizabeth Andrea
Jovin, Thomas M.
author2_role author
author
author
author
dc.subject.none.fl_str_mv Polarity Sensitive Dyes
Amyloid
Synuclein
https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
topic Polarity Sensitive Dyes
Amyloid
Synuclein
https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
description We introduce a sensor molecule, AS140-MFC, consisting of a covalent adduct of an Ala-to-Cys mutant of α-synuclein with the 3-hydroxychromone dual emission dye MFC. We show that the AS140-MFC construct is a multiparametric fluorescent probe suitable for the continuous monitoring of protein aggregation and is sensitive to the early and intermediate stages of α-synuclein aggregation, a process associated with Parkinsons disease.
publishDate 2010
dc.date.none.fl_str_mv 2010-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/68821
Yushchenko, Dmytro A.; Fauerbach, Jonathan Arturo; Thirunavukkuarasu, Shyamala; Jares, Elizabeth Andrea; Jovin, Thomas M.; Fluorescent ratiometric MFC probe sensitive to early stages of α-Synuclein aggregation; American Chemical Society; Journal of the American Chemical Society; 132; 23; 6-2010; 7860-7861
0002-7863
CONICET Digital
CONICET
url http://hdl.handle.net/11336/68821
identifier_str_mv Yushchenko, Dmytro A.; Fauerbach, Jonathan Arturo; Thirunavukkuarasu, Shyamala; Jares, Elizabeth Andrea; Jovin, Thomas M.; Fluorescent ratiometric MFC probe sensitive to early stages of α-Synuclein aggregation; American Chemical Society; Journal of the American Chemical Society; 132; 23; 6-2010; 7860-7861
0002-7863
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1021/ja102838n
info:eu-repo/semantics/altIdentifier/url/https://pubs.acs.org/doi/abs/10.1021/ja102838n
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Chemical Society
publisher.none.fl_str_mv American Chemical Society
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 15,812429

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