Progesterone attenuates astro and microgliosis and enhances oligodendrocyte differentittion following spinal cord injury

Reactive gliosis, demyelination and proliferation of NG2+ oligodendrocyte precursor cells (OPC) are common responses to spinal cord injury (SCI). We previously reported that short-term progesterone treatment stimulates OPC proliferation whereas chronic treatment enhances OPC differentiation after SC...

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Autores: Labombarda, Maria Florencia, Gonzalez, Susana Laura, Lima, Analia Ethel, Roig, Paulina, Gennoun, Rachida, Schumacher, Michael, de Nicola, Alejandro Federico
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2011
País:Argentina
Institución:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/14014
Acceso en línea:http://hdl.handle.net/11336/14014
Access Level:acceso abierto
Palabra clave:Progesterone
Spinal Cord Injuries
Reactive Gliosis
Oligodendroglia
Microglia
https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
id AR_8eb4fd8328d36bb383fa026c43ce5b8a
oai_identifier_str oai:ri.conicet.gov.ar:11336/14014
network_acronym_str AR
network_name_str Argentina
repository_id_str
dc.title.none.fl_str_mv Progesterone attenuates astro and microgliosis and enhances oligodendrocyte differentittion following spinal cord injury
title Progesterone attenuates astro and microgliosis and enhances oligodendrocyte differentittion following spinal cord injury
spellingShingle Progesterone attenuates astro and microgliosis and enhances oligodendrocyte differentittion following spinal cord injury
Labombarda, Maria Florencia
Progesterone
Spinal Cord Injuries
Reactive Gliosis
Oligodendroglia
Microglia
https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
title_short Progesterone attenuates astro and microgliosis and enhances oligodendrocyte differentittion following spinal cord injury
title_full Progesterone attenuates astro and microgliosis and enhances oligodendrocyte differentittion following spinal cord injury
title_fullStr Progesterone attenuates astro and microgliosis and enhances oligodendrocyte differentittion following spinal cord injury
title_full_unstemmed Progesterone attenuates astro and microgliosis and enhances oligodendrocyte differentittion following spinal cord injury
title_sort Progesterone attenuates astro and microgliosis and enhances oligodendrocyte differentittion following spinal cord injury
dc.creator.none.fl_str_mv Labombarda, Maria Florencia
Gonzalez, Susana Laura
Lima, Analia Ethel
Roig, Paulina
Gennoun, Rachida
Schumacher, Michael
de Nicola, Alejandro Federico
author Labombarda, Maria Florencia
author_facet Labombarda, Maria Florencia
Gonzalez, Susana Laura
Lima, Analia Ethel
Roig, Paulina
Gennoun, Rachida
Schumacher, Michael
de Nicola, Alejandro Federico
author_role author
author2 Gonzalez, Susana Laura
Lima, Analia Ethel
Roig, Paulina
Gennoun, Rachida
Schumacher, Michael
de Nicola, Alejandro Federico
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Progesterone
Spinal Cord Injuries
Reactive Gliosis
Oligodendroglia
Microglia
https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
topic Progesterone
Spinal Cord Injuries
Reactive Gliosis
Oligodendroglia
Microglia
https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
description Reactive gliosis, demyelination and proliferation of NG2+ oligodendrocyte precursor cells (OPC) are common responses to spinal cord injury (SCI). We previously reported that short-term progesterone treatment stimulates OPC proliferation whereas chronic treatment enhances OPC differentiation after SCI. Presently, we further studied the proliferation/differentiation of glial cells involved in inflammation and remyelination in male rats with SCI subjected to acute (3 days) or chronic (21 days) progesterone administration. Rats received several pulses of bromodeoyuridine (BrdU) 48 and 72 h post-SCI, and sacrificed 3 or 21 days post-SCI. Double colocalization of BrdU and specific cell markers showed that 3 days of SCI induced a strong proliferation of S100β+ astrocytes, OX-42+ microglia/macrophages and NG2+ cells. At this stage, the intense GFAP+ astrogliosis was BrdU negative. Twenty one days of SCI enhanced maturation of S100β+ cells into GFAP+ astrocytes, but decreased the number of CC1+ oligodendrocytes. Progesterone treatment inhibited astrocyte and microglia /macrophage proliferation and activation in the 3-day SCI group, and inhibited activation in the 21-day SCI group. BrdU/NG2 double labeled cells were increased by progesterone at 3 days, indicating a proliferation stimulus, but decreased them at 21 days. However, progesterone-enhancement of CC1+/BrdU+ oligodendrocyte density, suggest differentiation of OPC into mature oligondendrocytes. We conclude that progesterone effects after SCI involves: a) inhibition of astrocyte proliferation and activation; b) anti-inflammatory effects by preventing microglial activation and proliferation, and c) early proliferation of NG2+ progenitors and late remyelination. Thus, progesterone behaves as a glioactive factor favoring remyelination and inhibiting reactive gliosis.
publishDate 2011
dc.date.none.fl_str_mv 2011-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/14014
Labombarda, Maria Florencia; Gonzalez, Susana Laura; Lima, Analia Ethel; Roig, Paulina; Gennoun, Rachida; et al.; Progesterone attenuates astro and microgliosis and enhances oligodendrocyte differentittion following spinal cord injury; Elsevier Inc; Experimental Neurology; 231; 1; 9-2011; 135-146
0014-4886
1090-2430
url http://hdl.handle.net/11336/14014
identifier_str_mv Labombarda, Maria Florencia; Gonzalez, Susana Laura; Lima, Analia Ethel; Roig, Paulina; Gennoun, Rachida; et al.; Progesterone attenuates astro and microgliosis and enhances oligodendrocyte differentittion following spinal cord injury; Elsevier Inc; Experimental Neurology; 231; 1; 9-2011; 135-146
0014-4886
1090-2430
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0014488611002238
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.expneurol.2011.06.001
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Inc
publisher.none.fl_str_mv Elsevier Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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spelling Progesterone attenuates astro and microgliosis and enhances oligodendrocyte differentittion following spinal cord injuryLabombarda, Maria FlorenciaGonzalez, Susana LauraLima, Analia EthelRoig, PaulinaGennoun, RachidaSchumacher, Michaelde Nicola, Alejandro FedericoProgesteroneSpinal Cord InjuriesReactive GliosisOligodendrogliaMicrogliahttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Reactive gliosis, demyelination and proliferation of NG2+ oligodendrocyte precursor cells (OPC) are common responses to spinal cord injury (SCI). We previously reported that short-term progesterone treatment stimulates OPC proliferation whereas chronic treatment enhances OPC differentiation after SCI. Presently, we further studied the proliferation/differentiation of glial cells involved in inflammation and remyelination in male rats with SCI subjected to acute (3 days) or chronic (21 days) progesterone administration. Rats received several pulses of bromodeoyuridine (BrdU) 48 and 72 h post-SCI, and sacrificed 3 or 21 days post-SCI. Double colocalization of BrdU and specific cell markers showed that 3 days of SCI induced a strong proliferation of S100β+ astrocytes, OX-42+ microglia/macrophages and NG2+ cells. At this stage, the intense GFAP+ astrogliosis was BrdU negative. Twenty one days of SCI enhanced maturation of S100β+ cells into GFAP+ astrocytes, but decreased the number of CC1+ oligodendrocytes. Progesterone treatment inhibited astrocyte and microglia /macrophage proliferation and activation in the 3-day SCI group, and inhibited activation in the 21-day SCI group. BrdU/NG2 double labeled cells were increased by progesterone at 3 days, indicating a proliferation stimulus, but decreased them at 21 days. However, progesterone-enhancement of CC1+/BrdU+ oligodendrocyte density, suggest differentiation of OPC into mature oligondendrocytes. We conclude that progesterone effects after SCI involves: a) inhibition of astrocyte proliferation and activation; b) anti-inflammatory effects by preventing microglial activation and proliferation, and c) early proliferation of NG2+ progenitors and late remyelination. Thus, progesterone behaves as a glioactive factor favoring remyelination and inhibiting reactive gliosis.Fil: Labombarda, Maria Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Bioquímica Humana; ArgentinaFil: Gonzalez, Susana Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Bioquímica Humana; ArgentinaFil: Lima, Analia Ethel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Bioquímica Humana; ArgentinaFil: Roig, Paulina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Bioquímica Humana; ArgentinaFil: Gennoun, Rachida. Inserm; FranciaFil: Schumacher, Michael. Inserm; FranciaFil: de Nicola, Alejandro Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Bioquímica Humana; ArgentinaElsevier Inc2011-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/14014Labombarda, Maria Florencia; Gonzalez, Susana Laura; Lima, Analia Ethel; Roig, Paulina; Gennoun, Rachida; et al.; Progesterone attenuates astro and microgliosis and enhances oligodendrocyte differentittion following spinal cord injury; Elsevier Inc; Experimental Neurology; 231; 1; 9-2011; 135-1460014-48861090-2430enginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0014488611002238info:eu-repo/semantics/altIdentifier/doi/10.1016/j.expneurol.2011.06.001info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T14:27:26Zoai:ri.conicet.gov.ar:11336/14014instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 14:27:26.931CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
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