Adsorption and Electrooxidation of DNA at Glassy Carbon Electrodes Modified with Multiwall Carbon Nanotubes Dispersed in Glucose Oxidase
We are proposing for the first time the successful immobilization of DNA at glassy carbon electrodes (GCE) modified with carbon nanotubes (CNT) dispersed in glucose oxidase (GOx) (GCE/CNT-GOx) either by direct adsorption or by layer-by-layer self-assembling using polydiallyldimethylamine (PDDA). The...
| Autores: | , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2013 |
| País: | Argentina |
| Institución: | Consejo Nacional de Investigaciones Científicas y Técnicas |
| Repositorio: | CONICET Digital (CONICET) |
| Idioma: | inglés |
| OAI Identifier: | oai:ri.conicet.gov.ar:11336/25006 |
| Acceso en línea: | http://hdl.handle.net/11336/25006 |
| Access Level: | acceso abierto |
| Palabra clave: | CARBON NANOTUBES DISPERSION DNA-DRUG INTERACTION DOUBLE STRANDED CALF-THYMUS DNA GLASSY CARBON ELECTRODE GLUCOSE OXIDASE METHYLENE BLUE SELF-ASSEMBLING MULTILAYERS https://purl.org/becyt/ford/1.4 https://purl.org/becyt/ford/1 |
| Sumario: | We are proposing for the first time the successful immobilization of DNA at glassy carbon electrodes (GCE) modified with carbon nanotubes (CNT) dispersed in glucose oxidase (GOx) (GCE/CNT-GOx) either by direct adsorption or by layer-by-layer self-assembling using polydiallyldimethylamine (PDDA). The presence of GOx allows an efficient dispersion of CNT and gives a most favorable environment that promotes the adsorption and makes possible a more sensitive electrooxidation of DNA. The PDDA incorporated in the self-assembled architecture largely facilitates the adsorption and electrooxidation of dsDNA and the adsorbed layer can be successfully used for evaluating the interaction of DNA with methylene blue. |
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