Isolation and characterization of a novel electrogenic bacterium, Dietzia sp. RNV-4

Electrogenic bacteria are organisms that can transfer electrons to extracellular electron acceptors and have the potential to be used in devices such as bioelectrochemical systems (BES). In this study, Dietzia sp. RNV-4 bacterium has been isolated and identified based on its biochemical, physiologic...

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Detalhes bibliográficos
Autores: Sacco, Natalia Jimena, Bonetto, Maria Celina, Corton, Eduardo
Tipo de documento: artigo
Estado:Versão publicada
Data de publicação:2017
País:Argentina
Recursos:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositório:CONICET Digital (CONICET)
Idioma:inglês
OAI Identifier:oai:ri.conicet.gov.ar:11336/56957
Acesso em linha:http://hdl.handle.net/11336/56957
Access Level:Acceso aberto
Palavra-chave:ELECTROGENIC BACTERIUM
MICROBIOLOGY
MICROBIAL FUEL CELL
BIOSENSOR
https://purl.org/becyt/ford/2.8
https://purl.org/becyt/ford/2
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spelling Isolation and characterization of a novel electrogenic bacterium, Dietzia sp. RNV-4Sacco, Natalia JimenaBonetto, Maria CelinaCorton, EduardoELECTROGENIC BACTERIUMMICROBIOLOGYMICROBIAL FUEL CELLBIOSENSORhttps://purl.org/becyt/ford/2.8https://purl.org/becyt/ford/2Electrogenic bacteria are organisms that can transfer electrons to extracellular electron acceptors and have the potential to be used in devices such as bioelectrochemical systems (BES). In this study, Dietzia sp. RNV-4 bacterium has been isolated and identified based on its biochemical, physiological and morphological characteristics, as well as by its 16S rRNA sequence analysis. Furthermore, the current density production and electron transfer mechanisms were investigated using bioelectrochemical methods. The chronoamperometric data showed that the biofilm of Dietzia sp. RNV-4 grew as the current increased with time, reaching a maximum of 176.6 ± 66.1 mA/m2 at the end of the experiment (7 d); this highly suggests that the current was generated by the biofilm. The main electron transfer mechanism, indicated by the cyclic voltammograms, was due to secreted redox mediators. By high performance liquid chromatography, canthaxanthin was identified as the main compound involved in charge transfer between the bacteria and the solid electrodes. Dietzia sp. RNV-4 was used as biological material in a microbial fuel cell (MFC) and the current density production was 299.4 ± 40.2 mA/m2. This is the first time that Dietzia sp. RNV-4 has been electrochemically characterized and identified as a new electrogenic strain.Fil: Sacco, Natalia Jimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Bonetto, Maria Celina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Corton, Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; ArgentinaPublic Library of Science2017-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/56957Sacco, Natalia Jimena; Bonetto, Maria Celina; Corton, Eduardo; Isolation and characterization of a novel electrogenic bacterium, Dietzia sp. RNV-4; Public Library of Science; Plos One; 12; 2; 2-2017; 1-201932-6203CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0169955info:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0169955info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T13:55:01Zoai:ri.conicet.gov.ar:11336/56957instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 13:55:01.534CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Isolation and characterization of a novel electrogenic bacterium, Dietzia sp. RNV-4
title Isolation and characterization of a novel electrogenic bacterium, Dietzia sp. RNV-4
spellingShingle Isolation and characterization of a novel electrogenic bacterium, Dietzia sp. RNV-4
Sacco, Natalia Jimena
ELECTROGENIC BACTERIUM
MICROBIOLOGY
MICROBIAL FUEL CELL
BIOSENSOR
https://purl.org/becyt/ford/2.8
https://purl.org/becyt/ford/2
title_short Isolation and characterization of a novel electrogenic bacterium, Dietzia sp. RNV-4
title_full Isolation and characterization of a novel electrogenic bacterium, Dietzia sp. RNV-4
title_fullStr Isolation and characterization of a novel electrogenic bacterium, Dietzia sp. RNV-4
title_full_unstemmed Isolation and characterization of a novel electrogenic bacterium, Dietzia sp. RNV-4
title_sort Isolation and characterization of a novel electrogenic bacterium, Dietzia sp. RNV-4
dc.creator.none.fl_str_mv Sacco, Natalia Jimena
Bonetto, Maria Celina
Corton, Eduardo
author Sacco, Natalia Jimena
author_facet Sacco, Natalia Jimena
Bonetto, Maria Celina
Corton, Eduardo
author_role author
author2 Bonetto, Maria Celina
Corton, Eduardo
author2_role author
author
dc.subject.none.fl_str_mv ELECTROGENIC BACTERIUM
MICROBIOLOGY
MICROBIAL FUEL CELL
BIOSENSOR
https://purl.org/becyt/ford/2.8
https://purl.org/becyt/ford/2
topic ELECTROGENIC BACTERIUM
MICROBIOLOGY
MICROBIAL FUEL CELL
BIOSENSOR
https://purl.org/becyt/ford/2.8
https://purl.org/becyt/ford/2
description Electrogenic bacteria are organisms that can transfer electrons to extracellular electron acceptors and have the potential to be used in devices such as bioelectrochemical systems (BES). In this study, Dietzia sp. RNV-4 bacterium has been isolated and identified based on its biochemical, physiological and morphological characteristics, as well as by its 16S rRNA sequence analysis. Furthermore, the current density production and electron transfer mechanisms were investigated using bioelectrochemical methods. The chronoamperometric data showed that the biofilm of Dietzia sp. RNV-4 grew as the current increased with time, reaching a maximum of 176.6 ± 66.1 mA/m2 at the end of the experiment (7 d); this highly suggests that the current was generated by the biofilm. The main electron transfer mechanism, indicated by the cyclic voltammograms, was due to secreted redox mediators. By high performance liquid chromatography, canthaxanthin was identified as the main compound involved in charge transfer between the bacteria and the solid electrodes. Dietzia sp. RNV-4 was used as biological material in a microbial fuel cell (MFC) and the current density production was 299.4 ± 40.2 mA/m2. This is the first time that Dietzia sp. RNV-4 has been electrochemically characterized and identified as a new electrogenic strain.
publishDate 2017
dc.date.none.fl_str_mv 2017-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/56957
Sacco, Natalia Jimena; Bonetto, Maria Celina; Corton, Eduardo; Isolation and characterization of a novel electrogenic bacterium, Dietzia sp. RNV-4; Public Library of Science; Plos One; 12; 2; 2-2017; 1-20
1932-6203
CONICET Digital
CONICET
url http://hdl.handle.net/11336/56957
identifier_str_mv Sacco, Natalia Jimena; Bonetto, Maria Celina; Corton, Eduardo; Isolation and characterization of a novel electrogenic bacterium, Dietzia sp. RNV-4; Public Library of Science; Plos One; 12; 2; 2-2017; 1-20
1932-6203
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0169955
info:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0169955
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 15.198674

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