NMDA receptors contribute to the survival promoting effect of high potassium in cultured cerebellar granule cells

The present report further analyzes the survival promoting effect of high potassium, a condition that mimics neural activity in cultured cerebellar granule cells, an excellent model to study trophic mechanisms induced by depolarization and trophic factors. We found that the survival promoting effect...

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Detalles Bibliográficos
Autores: Armanino, María, Gravielle, Maria Clara, Marangoni, M. Natalia, Fiszman, Monica Lidia
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2005
País:Argentina
Institución:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/94599
Acceso en línea:http://hdl.handle.net/11336/94599
Access Level:acceso abierto
Palabra clave:BRAIN-DERIVED NEUROTROPHIC FACTOR
CEREBELLAR GRANULE CELLS
DEPOLARIZATION
DEVELOPMENT
NMDA
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
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network_acronym_str AR
network_name_str Argentina
repository_id_str
spelling NMDA receptors contribute to the survival promoting effect of high potassium in cultured cerebellar granule cellsArmanino, MaríaGravielle, Maria ClaraMarangoni, M. NataliaFiszman, Monica LidiaBRAIN-DERIVED NEUROTROPHIC FACTORCEREBELLAR GRANULE CELLSDEPOLARIZATIONDEVELOPMENTNMDAhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The present report further analyzes the survival promoting effect of high potassium, a condition that mimics neural activity in cultured cerebellar granule cells, an excellent model to study trophic mechanisms induced by depolarization and trophic factors. We found that the survival promoting effect measured at 7 days in vitro (DIV 7) of depolarizing potassium concentrations (25 mM KCl), added at DIV2, is partially prevented by adding at DIV 2 the non‐competitive NMDA blocker MK801 (10 μM). The concentration of MK801 used blocks completely the survival promoting effect of a supramaximal effective concentration of NMDA (100 μM). The addition at DIV 2 of anti‐brain derived neurotrophic factor (anti‐BDNF) antibody, failed to modify the effect of high potassium. The present report provides evidences that in cultured cerebellar granule cells, high potassium‐induced survival promoting effect is due in part by the activation of NMDA receptors. The effect does not require the presence of BDNF.Fil: Armanino, María. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Farmacológicas. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Investigaciones Farmacológicas; ArgentinaFil: Gravielle, Maria Clara. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Farmacológicas. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Investigaciones Farmacológicas; ArgentinaFil: Marangoni, M. Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Farmacológicas. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Investigaciones Farmacológicas; ArgentinaFil: Fiszman, Monica Lidia. University Of Georgetown; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Farmacológicas. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Investigaciones Farmacológicas; ArgentinaPergamon-Elsevier Science Ltd2005-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/94599Armanino, María; Gravielle, Maria Clara; Marangoni, M. Natalia; Fiszman, Monica Lidia; NMDA receptors contribute to the survival promoting effect of high potassium in cultured cerebellar granule cells; Pergamon-Elsevier Science Ltd; International Journal of Developmental Neuroscience; 23; 6; 10-2005; 545-5480736-5748CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1016/j.ijdevneu.2005.05.001info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ijdevneu.2005.05.001info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T13:52:31Zoai:ri.conicet.gov.ar:11336/94599instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 13:52:31.567CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv NMDA receptors contribute to the survival promoting effect of high potassium in cultured cerebellar granule cells
title NMDA receptors contribute to the survival promoting effect of high potassium in cultured cerebellar granule cells
spellingShingle NMDA receptors contribute to the survival promoting effect of high potassium in cultured cerebellar granule cells
Armanino, María
BRAIN-DERIVED NEUROTROPHIC FACTOR
CEREBELLAR GRANULE CELLS
DEPOLARIZATION
DEVELOPMENT
NMDA
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
title_short NMDA receptors contribute to the survival promoting effect of high potassium in cultured cerebellar granule cells
title_full NMDA receptors contribute to the survival promoting effect of high potassium in cultured cerebellar granule cells
title_fullStr NMDA receptors contribute to the survival promoting effect of high potassium in cultured cerebellar granule cells
title_full_unstemmed NMDA receptors contribute to the survival promoting effect of high potassium in cultured cerebellar granule cells
title_sort NMDA receptors contribute to the survival promoting effect of high potassium in cultured cerebellar granule cells
dc.creator.none.fl_str_mv Armanino, María
Gravielle, Maria Clara
Marangoni, M. Natalia
Fiszman, Monica Lidia
author Armanino, María
author_facet Armanino, María
Gravielle, Maria Clara
Marangoni, M. Natalia
Fiszman, Monica Lidia
author_role author
author2 Gravielle, Maria Clara
Marangoni, M. Natalia
Fiszman, Monica Lidia
author2_role author
author
author
dc.subject.none.fl_str_mv BRAIN-DERIVED NEUROTROPHIC FACTOR
CEREBELLAR GRANULE CELLS
DEPOLARIZATION
DEVELOPMENT
NMDA
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
topic BRAIN-DERIVED NEUROTROPHIC FACTOR
CEREBELLAR GRANULE CELLS
DEPOLARIZATION
DEVELOPMENT
NMDA
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
description The present report further analyzes the survival promoting effect of high potassium, a condition that mimics neural activity in cultured cerebellar granule cells, an excellent model to study trophic mechanisms induced by depolarization and trophic factors. We found that the survival promoting effect measured at 7 days in vitro (DIV 7) of depolarizing potassium concentrations (25 mM KCl), added at DIV2, is partially prevented by adding at DIV 2 the non‐competitive NMDA blocker MK801 (10 μM). The concentration of MK801 used blocks completely the survival promoting effect of a supramaximal effective concentration of NMDA (100 μM). The addition at DIV 2 of anti‐brain derived neurotrophic factor (anti‐BDNF) antibody, failed to modify the effect of high potassium. The present report provides evidences that in cultured cerebellar granule cells, high potassium‐induced survival promoting effect is due in part by the activation of NMDA receptors. The effect does not require the presence of BDNF.
publishDate 2005
dc.date.none.fl_str_mv 2005-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/94599
Armanino, María; Gravielle, Maria Clara; Marangoni, M. Natalia; Fiszman, Monica Lidia; NMDA receptors contribute to the survival promoting effect of high potassium in cultured cerebellar granule cells; Pergamon-Elsevier Science Ltd; International Journal of Developmental Neuroscience; 23; 6; 10-2005; 545-548
0736-5748
CONICET Digital
CONICET
url http://hdl.handle.net/11336/94599
identifier_str_mv Armanino, María; Gravielle, Maria Clara; Marangoni, M. Natalia; Fiszman, Monica Lidia; NMDA receptors contribute to the survival promoting effect of high potassium in cultured cerebellar granule cells; Pergamon-Elsevier Science Ltd; International Journal of Developmental Neuroscience; 23; 6; 10-2005; 545-548
0736-5748
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1016/j.ijdevneu.2005.05.001
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ijdevneu.2005.05.001
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 15.81155

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