Colonization of sorghum and wheat by seed inoculation with <i>Gluconacetobacter diazotrophicus</i>
Colonization of sorghum and wheat after seed inoculation with <i>Gluconacetobacter diazotrophicus</i> strains PAL 5 and UAP 5541/pRGS561 (containing the marker gene gusA) was studied by colony counting and microscopic observation of plant tissues. Inoculum levels as low as 10² CFU per se...
| Autores: | , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2010 |
| País: | Argentina |
| Institución: | Universidad Nacional de La Plata |
| Repositorio: | SEDICI (UNLP) |
| Idioma: | inglés |
| OAI Identifier: | oai:sedici.unlp.edu.ar:10915/144416 |
| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/144416 |
| Access Level: | acceso abierto |
| Palabra clave: | Ciencias Exactas Química Endophytes Gluconacetobacter diazotrophicus Inoculation Sorghum Wheat |
| Sumario: | Colonization of sorghum and wheat after seed inoculation with <i>Gluconacetobacter diazotrophicus</i> strains PAL 5 and UAP 5541/pRGS561 (containing the marker gene gusA) was studied by colony counting and microscopic observation of plant tissues. Inoculum levels as low as 10² CFU per seed were enough for root colonization and further spreading in aerial tissues. Rhizoplane colonization was around 7 log CFU g⁻¹ (fresh weight). <i>G. diazotrophicus</i> was found inside sorghum and wheat roots with populations higher than 5 log CFU g⁻¹ (fresh weight). Stem colonization remained stable for 30 days post inoculation with endophyte concentrations from 4 to 5 log CFU g⁻¹ (fresh weight) (in both plants). Population in leaves decreased continuously being undetectable after 17 days post inoculation. |
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