Transient gene expression in serum-free suspension-growing mammalian cells for the production of foot-and-mouth disease virus empty capsids

Foot-and-mouth disease (FMD) is a highly contagious disease of cloven-hoofed animals. It produces severe economic losses in the livestock industry. Currently available vaccines are based on inactivated FMD virus (FMDV). The use of empty capsids as a subunit vaccine has been reported to be a promisin...

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Bibliographic Details
Authors: Mignaqui, Ana Clara, Ruiz, Vanesa, Perret, Sylvie, St-Laurent, Gilles, Chahal, Parminder Singh, Transfiguracion, Julia, Sammarruco, Romina Ayelén, Gnazzo, Victoria, Durocher, Yves, Wigdorovitz, Andres
Format: article
Status:Published version
Publication Date:2013
Country:Argentina
Institution:Instituto Nacional de Tecnología Agropecuaria
Repository:INTA Digital (INTA)
Language:English
OAI Identifier:oai:localhost:20.500.12123/4809
Online Access:http://hdl.handle.net/20.500.12123/4809
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0072800
https://doi.org/10.1371/journal.pone.0072800
Access Level:Open access
Keyword:Expresión Génica
Mamíferos
Virus Fiebre Aftosa
Recombinación
Transfección
Vacuna
Proteasas
Gene Expression
Mammals
Aphthovirus
Recombination
Transfection
Vaccines
Proteases
Foot and Mouth Disease Virus
Capsids
Cápsidas
Description
Summary:Foot-and-mouth disease (FMD) is a highly contagious disease of cloven-hoofed animals. It produces severe economic losses in the livestock industry. Currently available vaccines are based on inactivated FMD virus (FMDV). The use of empty capsids as a subunit vaccine has been reported to be a promising candidate because it avoids the use of virus in the vaccine production and conserves the conformational epitopes of the virus. In this report, we explored transient gene expression (TGE) in serum-free suspension-growing mammalian cells for the production of FMDV recombinant empty capsids as a subunit vaccine. The recombinant proteins produced, assembled into empty capsids and induced protective immune response against viral challenge in mice. Furthermore, they were recognized by anti-FMDV bovine sera. By using this technology, we were able to achieve expression levels that are compatible with the development of a vaccine. Thus, TGE of mammalian cells is an easy to perform, scalable and cost-effective technology for the production of a recombinant subunit vaccine against FMDV.

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