Comparative proteomics of cerebrospinal fluid in neuropathologically- confirmed Alzheimer's disease and non-demented elderly subjects
Objectives: Diagnostic tests able to reveal Alzheimer's disease (AD) in living patients before cognitive ability is destroyed are urgently needed. Such tests must distinguish AD from other dementia causes, as well as differentiate subtle changes associated with normal aging from true pathology...
| Autores: | , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2006 |
| País: | Argentina |
| Institución: | Consejo Nacional de Investigaciones Científicas y Técnicas |
| Repositorio: | CONICET Digital (CONICET) |
| Idioma: | inglés |
| OAI Identifier: | oai:ri.conicet.gov.ar:11336/39061 |
| Acceso en línea: | http://hdl.handle.net/11336/39061 |
| Access Level: | acceso abierto |
| Palabra clave: | Alzheimer'S Disease Biomarkers Cerebrospinal Fluid Proteomics https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
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Comparative proteomics of cerebrospinal fluid in neuropathologically- confirmed Alzheimer's disease and non-demented elderly subjectsCastaño, Eduardo MiguelRoher, Alex E.Esh, Chera L.Kokjohn, Tyler A.Beach, ThomasAlzheimer'S DiseaseBiomarkersCerebrospinal FluidProteomicshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Objectives: Diagnostic tests able to reveal Alzheimer's disease (AD) in living patients before cognitive ability is destroyed are urgently needed. Such tests must distinguish AD from other dementia causes, as well as differentiate subtle changes associated with normal aging from true pathology emergence. A single biomarker offering such diagnostic and prognostic capacities has eluded identification. Therefore, a valuable test for AD is likely to be based on a specific pattern of change in a set of proteins, rather than a single protein. Methods: We examined pooled cerebrospinal fluid (CSF) samples obtained from neuropathologically-confirmed AD (n=43) and non-demented control subjects (n=43) using 2-dimensional gel electrophoresis (2DE) proteomic methodology to detect differentially expressed proteins. Proteins exhibiting expression level differences between the pools were recovered and identified using matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry. Results: Five differentially-expressed proteins with potential roles in amyloid-β metabolism and vascular and brain physiology [apolipoprotein A-1 (Apo A-1), cathepsin D (CatD), hemopexin (HPX), transthyretin (TTR), and two pigment epithelium-derived factor (PEDF) isoforms] were identified. Apo A-1, CatD and TTR were significantly reduced in the AD pool sample, while HPX and the PEDF isoforms were increased in AD CSF. Discussion: These results suggest that multi-factor proteomic pattern analysis of the CSF may provide a means to diagnose and assess AD. © 2006 W. S. Maney & Son Ltd.Fil: Castaño, Eduardo Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina. Sun Health Research Institute; Estados UnidosFil: Roher, Alex E.. Sun Health Research Institute; Estados UnidosFil: Esh, Chera L.. Sun Health Research Institute; Estados UnidosFil: Kokjohn, Tyler A.. Sun Health Research Institute; Estados UnidosFil: Beach, Thomas. Sun Health Research Institute; Estados UnidosManey Publishing2006-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/39061Castaño, Eduardo Miguel; Roher, Alex E.; Esh, Chera L.; Kokjohn, Tyler A.; Beach, Thomas; Comparative proteomics of cerebrospinal fluid in neuropathologically- confirmed Alzheimer's disease and non-demented elderly subjects; Maney Publishing; Neurological Research; 28; 2; 3-2006; 155-1630161-64121743-1328CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/abs/10.1179/016164106X98035info:eu-repo/semantics/altIdentifier/doi/10.1179/016164106X98035info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T14:09:01Zoai:ri.conicet.gov.ar:11336/39061instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 14:09:01.639CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
| dc.title.none.fl_str_mv |
Comparative proteomics of cerebrospinal fluid in neuropathologically- confirmed Alzheimer's disease and non-demented elderly subjects |
| title |
Comparative proteomics of cerebrospinal fluid in neuropathologically- confirmed Alzheimer's disease and non-demented elderly subjects |
| spellingShingle |
Comparative proteomics of cerebrospinal fluid in neuropathologically- confirmed Alzheimer's disease and non-demented elderly subjects Castaño, Eduardo Miguel Alzheimer'S Disease Biomarkers Cerebrospinal Fluid Proteomics https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
| title_short |
Comparative proteomics of cerebrospinal fluid in neuropathologically- confirmed Alzheimer's disease and non-demented elderly subjects |
| title_full |
Comparative proteomics of cerebrospinal fluid in neuropathologically- confirmed Alzheimer's disease and non-demented elderly subjects |
| title_fullStr |
Comparative proteomics of cerebrospinal fluid in neuropathologically- confirmed Alzheimer's disease and non-demented elderly subjects |
| title_full_unstemmed |
Comparative proteomics of cerebrospinal fluid in neuropathologically- confirmed Alzheimer's disease and non-demented elderly subjects |
| title_sort |
Comparative proteomics of cerebrospinal fluid in neuropathologically- confirmed Alzheimer's disease and non-demented elderly subjects |
| dc.creator.none.fl_str_mv |
Castaño, Eduardo Miguel Roher, Alex E. Esh, Chera L. Kokjohn, Tyler A. Beach, Thomas |
| author |
Castaño, Eduardo Miguel |
| author_facet |
Castaño, Eduardo Miguel Roher, Alex E. Esh, Chera L. Kokjohn, Tyler A. Beach, Thomas |
| author_role |
author |
| author2 |
Roher, Alex E. Esh, Chera L. Kokjohn, Tyler A. Beach, Thomas |
| author2_role |
author author author author |
| dc.subject.none.fl_str_mv |
Alzheimer'S Disease Biomarkers Cerebrospinal Fluid Proteomics https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
| topic |
Alzheimer'S Disease Biomarkers Cerebrospinal Fluid Proteomics https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
| description |
Objectives: Diagnostic tests able to reveal Alzheimer's disease (AD) in living patients before cognitive ability is destroyed are urgently needed. Such tests must distinguish AD from other dementia causes, as well as differentiate subtle changes associated with normal aging from true pathology emergence. A single biomarker offering such diagnostic and prognostic capacities has eluded identification. Therefore, a valuable test for AD is likely to be based on a specific pattern of change in a set of proteins, rather than a single protein. Methods: We examined pooled cerebrospinal fluid (CSF) samples obtained from neuropathologically-confirmed AD (n=43) and non-demented control subjects (n=43) using 2-dimensional gel electrophoresis (2DE) proteomic methodology to detect differentially expressed proteins. Proteins exhibiting expression level differences between the pools were recovered and identified using matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry. Results: Five differentially-expressed proteins with potential roles in amyloid-β metabolism and vascular and brain physiology [apolipoprotein A-1 (Apo A-1), cathepsin D (CatD), hemopexin (HPX), transthyretin (TTR), and two pigment epithelium-derived factor (PEDF) isoforms] were identified. Apo A-1, CatD and TTR were significantly reduced in the AD pool sample, while HPX and the PEDF isoforms were increased in AD CSF. Discussion: These results suggest that multi-factor proteomic pattern analysis of the CSF may provide a means to diagnose and assess AD. © 2006 W. S. Maney & Son Ltd. |
| publishDate |
2006 |
| dc.date.none.fl_str_mv |
2006-03 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
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article |
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publishedVersion |
| dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/39061 Castaño, Eduardo Miguel; Roher, Alex E.; Esh, Chera L.; Kokjohn, Tyler A.; Beach, Thomas; Comparative proteomics of cerebrospinal fluid in neuropathologically- confirmed Alzheimer's disease and non-demented elderly subjects; Maney Publishing; Neurological Research; 28; 2; 3-2006; 155-163 0161-6412 1743-1328 CONICET Digital CONICET |
| url |
http://hdl.handle.net/11336/39061 |
| identifier_str_mv |
Castaño, Eduardo Miguel; Roher, Alex E.; Esh, Chera L.; Kokjohn, Tyler A.; Beach, Thomas; Comparative proteomics of cerebrospinal fluid in neuropathologically- confirmed Alzheimer's disease and non-demented elderly subjects; Maney Publishing; Neurological Research; 28; 2; 3-2006; 155-163 0161-6412 1743-1328 CONICET Digital CONICET |
| dc.language.none.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/abs/10.1179/016164106X98035 info:eu-repo/semantics/altIdentifier/doi/10.1179/016164106X98035 |
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info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
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openAccess |
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https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
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application/pdf application/pdf |
| dc.publisher.none.fl_str_mv |
Maney Publishing |
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Maney Publishing |
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reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
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dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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