Oxidative imbalance in Candida tropicalis biofilms and its relation with persister cells

Persister cells (PCs) make up a small fraction of microbial population, can survive lethal concentrations of antimicrobial agents. In recent years, Candida tropicalis has emerged as being a frequent fungal agent of medical devices subject to biofilm infections. However, PCs are still poorly understo...

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Detalles Bibliográficos
Autores: Da Silva, María Angel, Baronetti, Jose Luis, Páez, Paulina Laura, Paraje, María Gabriela
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2021
País:Argentina
Institución:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/135937
Acceso en línea:http://hdl.handle.net/11336/135937
Access Level:acceso abierto
Palabra clave:ANTIFUNGALS
BIOFILMS
CANDIDA TROPICALIS
NITROSATIVE STRESS
OXIDATIVE STRESS
PERSISTER CELLS
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
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network_acronym_str AR
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repository_id_str
dc.title.none.fl_str_mv Oxidative imbalance in Candida tropicalis biofilms and its relation with persister cells
title Oxidative imbalance in Candida tropicalis biofilms and its relation with persister cells
spellingShingle Oxidative imbalance in Candida tropicalis biofilms and its relation with persister cells
Da Silva, María Angel
ANTIFUNGALS
BIOFILMS
CANDIDA TROPICALIS
NITROSATIVE STRESS
OXIDATIVE STRESS
PERSISTER CELLS
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
title_short Oxidative imbalance in Candida tropicalis biofilms and its relation with persister cells
title_full Oxidative imbalance in Candida tropicalis biofilms and its relation with persister cells
title_fullStr Oxidative imbalance in Candida tropicalis biofilms and its relation with persister cells
title_full_unstemmed Oxidative imbalance in Candida tropicalis biofilms and its relation with persister cells
title_sort Oxidative imbalance in Candida tropicalis biofilms and its relation with persister cells
dc.creator.none.fl_str_mv Da Silva, María Angel
Baronetti, Jose Luis
Páez, Paulina Laura
Paraje, María Gabriela
author Da Silva, María Angel
author_facet Da Silva, María Angel
Baronetti, Jose Luis
Páez, Paulina Laura
Paraje, María Gabriela
author_role author
author2 Baronetti, Jose Luis
Páez, Paulina Laura
Paraje, María Gabriela
author2_role author
author
author
dc.subject.none.fl_str_mv ANTIFUNGALS
BIOFILMS
CANDIDA TROPICALIS
NITROSATIVE STRESS
OXIDATIVE STRESS
PERSISTER CELLS
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
topic ANTIFUNGALS
BIOFILMS
CANDIDA TROPICALIS
NITROSATIVE STRESS
OXIDATIVE STRESS
PERSISTER CELLS
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
description Persister cells (PCs) make up a small fraction of microbial population, can survive lethal concentrations of antimicrobial agents. In recent years, Candida tropicalis has emerged as being a frequent fungal agent of medical devices subject to biofilm infections. However, PCs are still poorly understood. This study aimed to investigate the relation of PCs on the redox status in C. tropicalis biofilms exposed to high doses of Amphotericin B (AmB), and alterations in surface topography and the architecture of biofilms. Methods: We used an experimental model of two different C. tropicalis biofilms exposed to AmB at supra minimum inhibitory concentration (SMIC80), and the intra- and extracellular reactive oxygen species (iROS and eROS), reactive nitrogen species (RNS) and oxidative stress response were studied. Light microscopy (LM) and confocal laser scanning microscopy (CLSM) were also used in conjunction with the image analysis software COMSTAT. Results: We demonstrated that biofilms derived from the PC fraction (B2) showed a higher capacity to respond to the stress generated upon AmB treatment, compared with biofilms obtained from planktonic cells. In B2, a lower ROS and RNS accumulation was observed in concordance with higher activation of the antioxidant systems, resulting in an oxidative imbalance of a smaller magnitude compared to B1. LM analysis revealed that the AmB treatment provoked a marked decrease of biomass, showing a loss of cellular aggrupation, with the presence of mostly yeast cells. Moreover, significant structural changes in the biofilm architecture were noted between both biofilms by CLSM?COMSTAT analysis. For B1, the quantitative parameters bio-volume, average micro-colony volume, surface to bio-volume ratio and surface coverage showed reductions upon AmB treatment, whereas increases were observed in roughness coefficient and average diffusion distance. In addition, untreated B2 was substantially smaller than B1, with less biomass and thickness values. The analysis of the above-mentioned parameters also showed changes in B2 upon AmB exposure. Conclusion: To our knowledge, this is the first study that has attempted to correlate PCs of Candida biofilms with alterations in the prooxidant-antioxidant balance and the architecture of the biofilms. The finding of regular and PCs with different cellular stress status may help to solve the puzzle of biofilm resistance, with redox imbalance possibly being an important factor.
publishDate 2021
dc.date.none.fl_str_mv 2021-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/135937
Da Silva, María Angel; Baronetti, Jose Luis; Páez, Paulina Laura; Paraje, María Gabriela; Oxidative imbalance in Candida tropicalis biofilms and its relation with persister cells; Frontiers Media S.A.; Frontiers in Microbiology; 11; 2-2021; 1-14
1664-302X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/135937
identifier_str_mv Da Silva, María Angel; Baronetti, Jose Luis; Páez, Paulina Laura; Paraje, María Gabriela; Oxidative imbalance in Candida tropicalis biofilms and its relation with persister cells; Frontiers Media S.A.; Frontiers in Microbiology; 11; 2-2021; 1-14
1664-302X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.3389/fmicb.2020.598834
info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fmicb.2020.598834/full
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Frontiers Media S.A.
publisher.none.fl_str_mv Frontiers Media S.A.
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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spelling Oxidative imbalance in Candida tropicalis biofilms and its relation with persister cellsDa Silva, María AngelBaronetti, Jose LuisPáez, Paulina LauraParaje, María GabrielaANTIFUNGALSBIOFILMSCANDIDA TROPICALISNITROSATIVE STRESSOXIDATIVE STRESSPERSISTER CELLShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Persister cells (PCs) make up a small fraction of microbial population, can survive lethal concentrations of antimicrobial agents. In recent years, Candida tropicalis has emerged as being a frequent fungal agent of medical devices subject to biofilm infections. However, PCs are still poorly understood. This study aimed to investigate the relation of PCs on the redox status in C. tropicalis biofilms exposed to high doses of Amphotericin B (AmB), and alterations in surface topography and the architecture of biofilms. Methods: We used an experimental model of two different C. tropicalis biofilms exposed to AmB at supra minimum inhibitory concentration (SMIC80), and the intra- and extracellular reactive oxygen species (iROS and eROS), reactive nitrogen species (RNS) and oxidative stress response were studied. Light microscopy (LM) and confocal laser scanning microscopy (CLSM) were also used in conjunction with the image analysis software COMSTAT. Results: We demonstrated that biofilms derived from the PC fraction (B2) showed a higher capacity to respond to the stress generated upon AmB treatment, compared with biofilms obtained from planktonic cells. In B2, a lower ROS and RNS accumulation was observed in concordance with higher activation of the antioxidant systems, resulting in an oxidative imbalance of a smaller magnitude compared to B1. LM analysis revealed that the AmB treatment provoked a marked decrease of biomass, showing a loss of cellular aggrupation, with the presence of mostly yeast cells. Moreover, significant structural changes in the biofilm architecture were noted between both biofilms by CLSM?COMSTAT analysis. For B1, the quantitative parameters bio-volume, average micro-colony volume, surface to bio-volume ratio and surface coverage showed reductions upon AmB treatment, whereas increases were observed in roughness coefficient and average diffusion distance. In addition, untreated B2 was substantially smaller than B1, with less biomass and thickness values. The analysis of the above-mentioned parameters also showed changes in B2 upon AmB exposure. Conclusion: To our knowledge, this is the first study that has attempted to correlate PCs of Candida biofilms with alterations in the prooxidant-antioxidant balance and the architecture of the biofilms. The finding of regular and PCs with different cellular stress status may help to solve the puzzle of biofilm resistance, with redox imbalance possibly being an important factor.Fil: Da Silva, María Angel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: Baronetti, Jose Luis. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Páez, Paulina Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica; ArgentinaFil: Paraje, María Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFrontiers Media S.A.2021-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/135937Da Silva, María Angel; Baronetti, Jose Luis; Páez, Paulina Laura; Paraje, María Gabriela; Oxidative imbalance in Candida tropicalis biofilms and its relation with persister cells; Frontiers Media S.A.; Frontiers in Microbiology; 11; 2-2021; 1-141664-302XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.3389/fmicb.2020.598834info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fmicb.2020.598834/fullinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2024-05-08T14:28:56Zoai:ri.conicet.gov.ar:11336/135937instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982024-05-08 14:28:56.324CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
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